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1991 Fiscal Year Final Research Report Summary

Analyses of coloring Mechanism and Function of Retinal Proteins Using Model Proteins

Research Project

Project/Area Number 01480542
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionOsaka University

Principal Investigator

TOKUNAGA Fumio  Osaka University, Faculty of Science, Department of Biology Professor, 理学部, 教授 (80025452)

Project Period (FY) 1989 – 1991
KeywordsRetinal Proteins / Bacteriorhodopsin / Retinal Proteins / Retinol Binding Protein / Chromophore / Model Proteins / Visual Pigments
Research Abstract

Retinal proteins commonly have retianl as the chromophore but are different in the protein part so they have differnt color and function from each other. In this study we compared the colors and the functions among bactriorhodopisn, visual pigments, retinol-binding protein and retinochrome. We constructed artificial bacteriorhodopsins with partial peptides and compared the charactors with natural one. We could prepared an artificial bacteriorhodopsin wth chemically synthesized peptides corresponding to helices A and B and a peptide corresponding to helices C-G which was prepared from natural bacteriorhodopsin. The articial bacteriorhodopsin consisted of two species : One has the same color as the native one and the other has lambda_<max> around 480 nm. They were in a thermal equilibrium. Each pigment has own photocycle. These results suggest that amino acid residues in helices A andB play rolls for controlling color of bacteriorhodopsin. For promoting this series of experiments, we set … More up the expression system of partial peptides of bacteriorhodopsin in E. coli by recombinant DNA techniques and have already obtained some peptide fragments. By x-ray diffraction experiments we proved that helices B and G moved on functioning. We compared the amino acid sequences and the colors of visual pigments among various animals. The results strongly suggests that visual pigment genes of vertebrates have fundamentally evolved along five lines and that the amino acid residues which are conserved in visual pigments belonging to each line play some rolls for the expression of the colo. We performed experiments of retinal binding with beta lactogloblin which has the similar beta barrel structure to retinol-binding protein, but we could not detect the obvious evidences showing the b ing. Next we tried to bind retinal Schiff base and the protonated Schiff base which were made with retinal and butylamine, but the binding constants were small, so we have to improve the experiment systems. It was found that retinochorme has a protonated Schiff base as the same as other retinal proteins proteins and that carboxyl groups are deprotonated on functioning. Less

  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Hisatomi,O.,Iwasa,T.,Tokunaga,F.and Yasui,A.: "Isolation and Characterization of lomprey rhodopsin cDNA." Biochem,Biophys.Res.Commun.,. 174. 1125-1132 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasa,T.,Nakajima,K.Takao,M.and Tokunaga,F.: "Light-dependent proton movement and photointermediates on bacteriorhodopsin photocycle." Sci.Rep.Tohoku Univ.11. 229-237 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sekiya,N.,Kishigami,A.Naoki,H.,Chang,C.-W.Yoshihara,K.,Hara,R,Hara,T.and Tokunaga,F.: "Fourier Transform infrared spectroscopic study of retinochrome and its primary photoproduct lumirefinochrome." FEBS lett.280. 107-111 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 徳永 史生: "異常条件下の生物ー高度好塩菌と紫膜" 化学と工業. 44. 1113-1117 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 久富 修,徳永 史生: "PCR法の実際と視物質遺伝子のクロ-ニング" 生物物理. 31. 161-163 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 徳永 史生: "バクテリオロドプシンの構造モデル" 化学. 46. 438-439 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga, F., Iwasa, T., Takao, M., Sato, S. and Takeuchi, T.: "Preparation and properties of monoclonal antibodies against bovine rhodopsin." Zool. Sci.6. 167-171 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokutomi, S., Nakasako, M., Sakai, J., Kataoka, M., Yamamoto, K. T., Wada, M., Tokunaga, F. and Furuya, M.: "A model for the dimeric molecular structure of phytochrome based on small-angle X-ray scattering." FEBS Lett.247. 139-142 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horiuchi, S., Kitani, H., Koshida, Y., Tokunaga, F. and Takeuchi, T.: "Immunofluorescent staining of visual cells from various species by mouse monoclonal antibodies against bovine rhodopsin." Zool. Sci.6・1. 31-34 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, M., Watanabe, H., Koike, Y., Tonosaki, A., Hisatomi, O. and Tokunaga, F.: "Demonstration of rod and cone photoreceptors in the lamprey retina by lectin cytochemistry." Cell Tissue Res.256. 227-232 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakasako, M., Kataoka, M. and Tokunaga, F.: "Arginine remarkably prolongs the lifetime of the M-intermediate in the bacteriorhodopsin photocycle at room temperature." FEBS Lett.254. 211-214 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisatomi, O and Tokunaga, F.: "Visual pigment of lamprey (II)" Zool. Sci.6. 1084 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horiuchi, S., Kobayashi, H., Morita, T., Koshida, Y., and Tokunaga, F.: "Comparison of immunoreactivity in rod outer segments among vertebrates using anti-cattle rhodopsin monoclonal antibodies." Zool. Sci.6. 1198 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F.: "Functional proteins acting in vision." Animal Physiol.6. 227 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F.: "Studies on bacteriorhodopsin and halorhodopsin of halobacteria by gene manipulation" Proteins Nucleic Acids Enzymes. 34. 505-517 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F. and Iwasa, T.: "From DNA sequences of human color visual pigments" Biophys.29. 311-315 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F., Watanabe, T., Uematsu, J., Hara, R. and Hara, T.: "Photoreactions of retinochrome at very low temperatures." FEBS Lett.262. 266-268 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakasako, M., Wada, M., Tokutomi, S., Tamamoto, K. T., Sakai, J., Kataoka, M., Tokunaga, F. and Furuya, M.: "Quaternary structure of pea phytochrome I dimer studied with small-angle X-ray scattering and rotary-shadowing electron microscopy" Photochem. Photobiol.52. 3-12 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F., Iwasa, T. Miyagishi, M. and Kayada, S.: "Cloning of cDNA and amino acid sequence of one of chicken cone visual pigments" Biochem. Biophys, Res. Commun.173. 1212-1217 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagatani, H., Ozaki, M., Tokunaga, F. and Ozaki, K.: "The effect of light on the opsin synthesis in the eye of Drosophila opsin" Zool. Sci.7. 1029 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F., Iwasa, T., Miyagishi, M. and Kayada, S.: "Cloning of chicken genomic DNA molecules encoding visual pigments" Zool. Sci.7. 1030 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kishigami, A., Sekiya, N., Yoshihara, k., Hara, R., Hara, T. and Tokunaga, F.: "Structure in the protein moiety of retinochrome and its derivatives" Zool. Sci.7. 1032 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozaki, M., Ozaki, K. and Tokunaga, F.: "Chemosensillum of the blowfly Phormia regina : Isolation and protein composition" Zool. Sci.7. 1035 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F., Hisatomi, O., Iwasa, T. and Yasui, A.: "Visual pigment of lamprey" Comp. Physiol. Biochem.6. 194 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F. and Hisatomi, O.: "Reception and transduction of light stimuli" Petrotech. 13. 247-254 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F.: "Structure and expression of genes of photoreceptive substances" Cells. 22. 123-127 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisatomi, O., Iwasa, T., Tokunaga, F. and Yasui, A.: "Isolation and Characterization of lamprey rhodopsin cDNA" Biochem, . Biophys. Res. Commun.174. 1125-1132 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasa, T., Nakajima, K., Takao, M. and Tokunaga, F.: "Light-dependent proton movement and photointermediates on bacteriorhopsin photocycle" Sci. Rep. Tohoku Univ.11. 229-237 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka, M., Sato, N., Hamanaka, T., Amemiya, Y. and Tokunaga, F.: "Properties of disorder induced in the purple membrane structure by iodination" Sci. Rep. Tohoku Univ.11. 239-250 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekiya, N., Kishigami, A., Naoki, H., Chang, C. -W., Yoshihara, K., Hara, R., Hara, T. and Tokunaga, F.: "Fourier transform infrared spectroscopic study of retinochrome and its primary photoproduct, lumiretinochrome" FEBS lett.280. 107-111 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagatani, H., Ozaki, M., Tokunaga, F. and Ozaki, K.: "Chromophoredependent maturation of Drosophila opsin" Zool. Sci.8. 1033 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hariyama, T., Ozaki, K., Tokunaga, F. and Tsukahara, Y.: "Cloning and sequecing of crayfish opsin cDNA" Zool. Sci.8. 1035 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisatomi, O., Kayada, S., Aoki, Y., Iwasa, T. and Tokunaga, F.: "Classification of visual pigment genes of vertebrates" Zool. Sci.8. 1035 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozaki, M., Amakawa, T., ozaki, K. and Tokunaga, F.: "Two functional different sugar receptor proteins found in the labellum of the blowfly Phormia regina" Zool. Sci.8. 1047 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F.: "Life under the unusual conditions--Halobacteria and bacteriorhodopsin" Chem. Indust.44. 1113-1117 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, F. and Hisatomi, O.: "Practice of PCR method and cloning of visual pigemnt genes" Biophys.31. 161-163 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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