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1990 Fiscal Year Final Research Report Summary

Permanent Effect of Hormones and Their Related Compounds on Target Organs

Research Project

Project/Area Number 01540618
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionYokohama City University

Principal Investigator

IGUCHI Taisen  Department of Biology, Yokohama City University (Associate professor), 文理学部, 助教授 (90128588)

Co-Investigator(Kenkyū-buntansha) TAKASUGI Noboru  Yokohama City University (President), 学長 (90011486)
Project Period (FY) 1989 – 1990
KeywordsDiethylstilbestrol / Irreversible change / Polyovular follicles / Tamoxifen / Critical period / Sexual dimorphism / Pelvis / Blood glucose
Research Abstract

Permanent effects of hormones and their related compounds on gonadーaccessory and neoplastic tissues were studied.
1. Tamoxifen. (1) Tamoxifen given neonatally induced degeneration of uterine glands, myometrium and oocytes in females, and (2) suppression of fibrocartilage formation in penis and suppression of spermatogenesis in males. (3) Critical periods of induction by tamoxifen of abnormalities in female and male mice were 7 and 3 days after birth, respectively. (4) Tamoxifen directly suppressed the ossification of pelvis in vitro.
2. Sexual dimorphism of pelvis. (1) Sexual dimorphism in pelvis was formed by 30 days of age. Pubis in females is longer and thinner than the males. Ischium in males is wider and larger than the females. (2) Studies on pelvis of Tfm mouse and sequential gonadectomy revealed that shapes of pubis and ischium were controlled by estrogen and androgen, respectively.
3. Polyovular follicles. (1) Polyovular follicles induced by neonatal treatment with diethylstilbestol (DES) can be ovulated by gonadotropins and (2) ova from polyovular follicles can be fertilized in vitro, though the fertilization rate is lower than those of ova from uniovular follicles from controls and neonatally DES-exposed mice.
4. Tumor and blood glucose. (1) Mice developed mammary tumor showed higher blood glucose and insulin than mice without the tumor. There is a positive correlation between mammary tumor size and blood glucose. In these mice, liver was larger than the controls. (2) Mice transplanted Lewis lung carcinoma showed higher blood glucose.
5. Protein profiles in irreversibly changed vagina. Two dimensional electrophoresis reveled that similar proteins were expressed both in vagina of ovariectomized mice treated neonatally with DES and in ovariectomized adult mice given injections of DES just before sacrifice. These results indicate that some proteins were permanently expressed in DES-exposed vagina.

  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Iguchi,T.,Todoroki,R.,Yamaguchi,S.and Takasugi,N.: "Changes in the uterus and vagina of mice treated neonatally with antiestrogens." Acta Anatomica. 136. 146-154 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohta,Y.,Iguchi,T.and Takasugi,N.: "Deciduoma formation in rats treated neonatally with antiーestrogens,tamoxifen and MERー25." Reproductive Toxicology. 3. 207-212 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi,T.,Irisawa,S.,Fukazawa,Y.,Uesugi,Y.and Takasugi,N.: "Morphometric analysis of the development of sexual dimorphism of the mouse pelvis." The Anatomical Record. 224. 490-494 (1889)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi,T.,Takasugi,N.,Nishimura,N.,and Kusunoki,S.: "Correlation between mammary tumor and blood glucose,serum insulin and free fatty acids in mice" Cancer Research. 49. 821-825 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Irisawa,S.,and Iguchi,T.: "Critical period of induction by tamoxifen of genital organ abnormalities in female mice." In Vivo. 4. 175-180 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Irisawa,S.,Iguchi,T.and Takasugi,N.: "Critical period of induction by tamoxifen of genital organ abnormalities in male mice." Zoological Science. 7. 541-545 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi,T.,Irisawa,S.,Uesugi,Y.,Kusunoki,S.,and Takasugi,N.: "Abnormal development of the os penis in male mice treated neonatally with tamoxifen." Acta Anatomica. 139. 201-208 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi,T.,Fukazawa,Y.,Uesugi,Y.,and Yakasugi,N.: "Polyovular follicles in mouse ovaries exposed neonatally to diethylstilbestrol in vivo and in vitro." Biology of Reproduction. 43. 478-484 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi,T.,Fukazawa,Y.,Tani,N.,Sato,T.,Ozawa,S.,Takasugi,N.et al.: "Effect of some hormonally active steroids upon the growth of LNCaP human prostate tumour cells in vitro." The Cancer Journal. 3. 184-191 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchima,F.ーD.A.,Edery,Iguchi,T.,and Bern,H.A.: "Growth of mouse endometrial luminal epithelial cells in vitro:functional integrity of the oestrogen receptor system and failure of oestrogen to induce proliferation." Journal of Endocrinology. 128. 115-120 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi,T.,Uesugi,Y.,Takasugi,N.and Petrow,V.: "Quantitative analysis of the development of genital organs from the urogenital sinus of the fetal male mouse treated prenatally with a 5αーreductase inhibitor." Journal of Endocrinology. 128. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi,T.,Uesugi,Y.,Sato,T.,Ohta,Y.and Takasugi,N.: "Developmental pattern of estrogen receptor expression in male mouse genital organs." Molecular Andrology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi,Y.,Reszka,A.Iguchi,T.,Reinish,B.and Horwitz,A.F.: "Expression of chicken integrin β1 subunit in rat PC12 cells." Cell Structure and Function. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井口 泰泉: "周生期の性ホルモン投与によって誘起されたマウス卵巣の多卵性濾胞" 横浜市立大学論叢自然科学系列. 41. 149-162 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 林 要喜知、井口 泰泉: "インテグリンの構造と機能" 横浜市立大学論叢自然科学系列. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi,T.and Uchima,F.ーD.A.: "Protocols in Cell&Tissue Culture" John Wiley&Sons, (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iguchi, T., R. Todoroki, S. Yamaguchi and N. Takasugi: "Changes in the uterus and vagina of mice treated neonatally with antiestrogens." Acta Anat.136. 146-154 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohta, Y., T. Iguchi and N. Takasugi: "Deciduoma formation in rats treated neonatally with anti-estrogens, tamoxifen and MER-25." Reprod. Toxicol.3. 207-212 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iguchi, T., S. Irisawa, Y. Fukazawa, Y. Uesugi and N. Takasugi: "Morphometric analysis of the development of sexual dimorphism of the mouse pelvis." Anat. Rec.224. 490-494 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iguchi, T., N. Takasugi, N. Nishimura and S. Kusunoki: "Correlation between mammary tumor and blood glucose, serum insulin and free fatty acids in mice." Cancer Res.49. 821-825 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Irisawa, S. and T. Iguchi: "Critical period of induction by tamoxifen of genital organ abnormalities in female mice." In Vivo. 4. 175-180 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Irisawa, S., T. Iguchi and N. Takasugi: "Critical period of induction by tamoxifen of genital organ abnormalities in male mice." Zool. Sci.7. 541-545 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iguchi, T., S. Irisawa, Y. Uesugi, S. Kusunoki and N. Takasugi: "Abnormal development of the os penis in male mice treated neonatally with tamoxifen." Acta Anat.139. 201-208 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iguchi, T., Y. Fukazawa, Y. Uesugi and N. Takasugi: "Polyovular follicles in mouse ovaries exposed neonatally to diethylstilbestrol in vivo and in vitro." Biol. Reprod.43. 478-484 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iguchi, T., Y. Fukazawa, N. Tani, T. Sato, S. Ozawa, N. Takasugi, T. Shuin, Y. Kubota and V. Petrow: "Effect of some hormonally active steroids upon the growth of LNCaP human prostate tumor cells in vitro." Cancer J.3. 184-191 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchima, F. -D. A., M. Edery, T. Igichi and H. A. Bern: "Growth of mouse endometrial luminal epithelial cells in vitro : functional integrity of the estrogen receptor system and failure of estrogen to induce proliferation." J. Endocr.128. 115-120 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iguchi, T., Y. Uesugi, N. Takasugi and V. Petrow: "Quantitative analysis of the development of genital organs from the urogenital sinus of the fetal male mouse treated prenatally with a 5alpha-reductase inhibitor." J. Endocr.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iguchi, T., Y. Uesugi, T. Sato, Y. Ohta and N. Takasugi: "Developmental pattern of estrogen receptor expression in male mouse genital organs." Mol. Androl.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi, Y., A. Reszka, T. Iguchi, B. Reinish and A. F. Horwitz: "Expression of chicken integrin beta_1 subunit in rat PC12 cells." Cell Struct. Funct.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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