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1991 Fiscal Year Final Research Report Summary

Induction of Genetic Variants for Soybean Seed Storage Proteins by Mutagenesis

Research Project

Project/Area Number 01560001
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionIwate University

Principal Investigator

KAIZUMA Norihiko  Fac. Agri., Iwate University Professor, 農学部, 教授 (00003773)

Co-Investigator(Kenkyū-buntansha) TAKAHATA Yosihito  Fac. Agri., Iwate University Assoc. Professor, 農学部, 助教授 (10133894)
Project Period (FY) 1989 – 1991
KeywordsSoybean / Seed Storage Proteins / Mutagenesis / 7S Globulin / 11S Globulin / Protein Lacking Variants / Chlorosis / Sulfur-Containing Amino Acid Content
Research Abstract

Nutritional improvement of soybean seed storage proteins can be realized by increase of sulfur-containing amino acid contents in the proteins. For that purpose it is the most efficient to decrease 7S globulin, one of the two major constituents of the proteins. Therefore, we performed induction experiments of a specific mutant gene(s)which lacked alpha and/or beta subunits proteins from 7S globulin by gamma-ray irradiation to dry seeds of soybean cultivar.
As a result, we succeeded in finding out a mutant gene which lacked both of the alpha and beta subunit proteins through SDS-PAGE analyses to the M4 seed progenies of 40-KR gamma-ray irradiated " WASESUZUNARI ". We confirmed that(1)the homozygous genotypes of the mutant gene were killed by chlorosis in seedling stage, (2)the heterozygous genotypes were viable and set seeds normally, and(3)the segregation ratio of the normal : intermediate for the alpha and beta subunit proteins in quantity : the lacking for both of the subunits was 3 : 4 : 1.
Moreover, we found out another mutant gene which lacked so-called " group 1 " subunits of 11S globulin(acidic subunits of Al, A2, A3)in the same gamma-ray irradiated progenies. This mutant gene worked as a recessive in one locus. The segregation ratio was 1 normal : 2 intermediate : 1 lacking. The homozygous genotypes(Al, A2, and A3 lacking)showed no abnormality in plant growth and set seeds normally.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 小田中 浩哉,海妻 矩彦: "ダイズ種子貯蔵タンパク質7Sグロブリンの突然変異体の簡易検定法について" 育種学雑誌. 38(別2). 238-239 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小田中 浩哉,海妻 矩彦: "放射線によって誘発された大豆種子貯蔵タンパク質に関する遺伝的変異体について" 育種学雑誌. 39(別1). 430-431 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 海妻 矩彦,小綿 寿志,小田中 浩哉: "放射線によって誘発された大豆種子貯蔵タンパク質に関する遺伝的変異体についてII、毛振品種からの変異体" 育種学雑誌. 39(別2). 148-149 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 海妻 矩彦,小田中 浩哉,佐藤 弘一,小綿 寿志: "放射線によって誘発された大豆種子貯臓タンパク質に関する遺伝的変異体についてIII11Sグロブリン変異体の蛋白質含量及び油脂含量" 育種学雑誌. 40(別1). 504-505 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 海妻 矩彦,ファントンフウ,高畑 義人: "放射線によって誘発された大豆種子貯臓タンパク質に関する遺伝的変異体についてIVβーコングリシニンタンパク質変異の遺伝様式について" 育種学雑誌. 40(別2). 208-209 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金 倫範,加藤 松三,海妻 矩彦,高畑 義人: "大豆種子貯臓タンパク質変異体遺伝子の中国大豆への導入" 育種学雑誌. 41(別1). 236-237 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Odanaka, H. and N. Kaizuma: "A simple and rapid test of soybean mutants on 7S globulin subunits" Japan. J. Breed.38(Suppl. 2). 238-239 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Odanaka, H. and N. Kaizuma: "* Mutants on soybean storage proteins induced with gamma-ray irradiation" Japan. J. Breed.39(Suppl. 1). 430-421 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaizuma, N., H. Kowata and H. Odanaka: "* II. Mutants from Keburi cultivar" Japan. J. Breed.39(Suppl. 2). 148-149 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaizuma, N., H. Odanaka, K. Sato and H. Kowata: "* III. Crude protein and oil contents of a mutant strain on 11S globulin subunits" Japan. J. Breed.40(Suppl. 1). 504-505 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaizuma, N., Phan Ton Huu, and Y. Takahata: "* IV. Mode of inheritance for a mutant of beta-conglicinin subunit protein proteins" Japan. J. Breed.40(Suppl. 2). 208-209 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jin, L., M. Kato, N. Kaizuma, and Y. Takahata: "Introduction of mutant genes of soybean seed storage protein to chinese va riety" Japan. J. Breed.41(Suppl. 1). 236-237 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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