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1990 Fiscal Year Final Research Report Summary

Chemical Studies on the Infection Mechanism of Crown Gall Disease

Research Project

Project/Area Number 01560152
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionOkayama University

Principal Investigator

KAWAZU Kazuyoshi  Okayama Univ., Fac. Agriculture, Professor, 農学部, 教授 (30026520)

Co-Investigator(Kenkyū-buntansha) KANZAKI Hiroshi  Okayama Univ., Grad. School, Research Associate, 大学院・自然科学研究科, 助手 (60183787)
Project Period (FY) 1989 – 1990
KeywordsCrown gall / Potato disk method / Plant transformation inhibition / Julichrome Q1・3 / Julimycine B-II / Agrobacterium tumefaciens / Octopine / Nopaline
Research Abstract

1. Agrobacterium tumefaciens is a soil bacterium which induces crown gall tumors in a wide variety of dicotyledonous plants. This tumorigenic interaction between the bacterium and the plant is a natural example of the genetic engineering of eukaryotes. Indeed, the oncogenic bacterium harbors a large Ti plasmid, whose T-DNA region is transferred to and integrated into the plant genome during tumorous transformation. However, very little information is available concerning the early process leading to tumor formation. In order to study further the mechanism of tumor induction, it is necessary to develop a chemical probe that will help in exploring the early steps of tumor formation. We searched transformation inhibitors which do not inhibit the growth of A. tumefaciens and are nontoxic against the transformed plant cells.
2. A few esters derived from oxazolomycin isolated from Streptomyces sp. KBFP-2025 as well as two active compounds of bisanthraquinone type, julichrome Q_<1・3> and julimycin B-II, isolated from Streptomyces sp. TM-71, inhibit crown gall formation, but are nontoxic against this bacterium and the host plant.
3. These compounds administered to a potato disk 12 - 24 hours after the inoculation of the bacterium hardly inhibited crown gall formation, and no longer necrosed the potato disk. This suggests that these compounds act on any step of transformation process, but have no toxicity toward transformed plant cells whose genome has integrated the T-DNA of A. tumefaciens. It is probable that the transformation process of this system is completed within 12 - 24 hours after the inoculation. For a direct proof of this hypothesis, a quantitative microanalytical method of opines, indices of transformation, has to be established. This method involves HPLC analysis of fluorescent compound formed by reaction of benzoin with guanidino group of the characteristic amino acid. Time course of transformation will be possibly traced by this method.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Satoru Kawai: "Inhibitory Effect of Oxazolomycin on Crown Gall Formation" Agric.Biol.Chem.53. 1127-1133 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Kawai: "A bacterial extracellular polysaccharide which enhances the attachment of Agrobacterium tumefaciens to the plant cell surface" Experientia. 45. 201-202 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuyoshi Kawazu: "Isolation and Structure of Neoannonin,a Novel Insecticidal Compound from the Seeds of Annona squamosa" Agric.Biol.Chem. 53. 2719-2722 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoru Kawai, Akio Kobayashi, Kazuyoshi Kawazu: "A bacterial extracellular polysaccharide which enhances the attachment of Agrobacterium tumefaciens to the plant cell surface" Experimental. 45. 201-202 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoru Kawai, Goro Kawabata, Akio Kobayashi, Kazuyoshi Kawazu: "The inhibitory effect of oxazolomycin on crown gall formation" Agr. Bio. Chem.53(4),. 1127-1133 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuyoshi Kawazu, Jocelyn P. Alcantara, Akio Kobayashi: "Isolation and structure of neoannonin, a novel insecticidal compound from the seeds of Annona squamosa" Agr. Biol. Chem.53(10). 2719-2722 (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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