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1990 Fiscal Year Final Research Report Summary

Degradation of Lignin Glycosides by Lignin Oxidizing Enzymes

Research Project

Project/Area Number 01560188
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 林産学
Research InstitutionKyushu University

Principal Investigator

KONDO Ryuichiro  Faculty of Agriculture, Kyusyu University. Associate Prof., 農学部, 助教授 (80091370)

Project Period (FY) 1989 – 1990
KeywordsLignin / Lignin Glycosides / DHP / DHP Glycosides / Lignin Peroxidase / Phanerochaete chrysosporium / white-rot fungi / Lignin biodegradation
Research Abstract

In order to clarify the effect of the formation of glycoside on the degradation of lignin by enzyme systems, DHP and DHP-glucoside were treated with horseradish peroxidase, laccase and lignin peroxidase. During the oxidation, the reaction mixture of DHP changed color from yellowish to dark brown and finally formed precipitates. On the other hand, the reaction mixture of DHP-Glc just changed color from yellowish to pale brown and formed no precipitate at all. The molecular weight distribution of the control and enzyme treated samples was analyzed by gel filtration chromatography. All molecular weight distribution patterns obtained here showed clearly that oxidation of DHP by enzyme systems containing laccase or peroxidase resulted in polymerization and not degradation of DHP.
On the other hand, when DHP-Glc was treated with the enzymes, enzyme-catalyzed depolymerization was observed. Under treatment conditions employed here, DHP-Glc was degraded more extensively into low molecular weight fractions by peroxidase treatment (horseradish peroxidase and lignin peroxidase) rather than laccase treatment (commercial laccase and laccase III). In the present study, we have observed that glucosidic DHP lignin can be degraded by peroxidase and laccase treatments. Although the mechanism for the possible degradation of lignin glycosides in vitro is not elucidated, the present results suggested that there must exist a system where the formation of glycoside prevents polymerization and accelerates efficient degradation reactions.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] R.Kondo,T.Iimori,H.Imamura,T.Nishida: "Polymerization of DHP and depolymerization of DHPーglucoside by lignin oxidizing enzymes" Journal of Biotechnology. 13. 181-188 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R. Kondo, T. Iimori, H. Imamura, T. Nishida: "Polymerization of DHP and depolymerization of DHP-glucoside by lignin oxidizing enzymes" J. Biotechnology. 13. 181-188 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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