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1990 Fiscal Year Final Research Report Summary

Possibility of Electron Spin Resonance Spectroscopy as a Diagnostic Technique in Immunodeficiency Syndrome of Domestic Animals

Research Project

Project/Area Number 01560333
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

KUWABARA Mikinori  Hokkaido University, Faculty of Veterinary Medicine, Associate Professor, 獣医学部, 助教授 (10002081)

Co-Investigator(Kenkyū-buntansha) ENDOH Daiji  Hokkaido University, Faculty of Veterinary Medicine, Instructor, 獣医学部, 助手 (40168828)
Project Period (FY) 1989 – 1990
KeywordsImmunodeficiency Syndrome / Granulocyte Hypofunction Syndrome / neutrophil / Superoxide / Electron Spin Resonance Spectroscopy / Spin Trapping Method / NADPH Oxidase / Phorbol 12-Myristate 13-Acetate
Research Abstract

Hypofunction of neutrophil cells is thought to be a cause of immunodeficiency syndrome of domestic animals. Since a function of neutrophil cells has been proved to be closely related to their ability to generate reactive oxygens, a method combining electron spin resonance spectroscopy (ESR) and spin trapping method (ST) was employed to detect and identify the reactive oxygens from neutrophil cells. On the basis of the results obtained, this EST-ST method was judged to be or not to be an appropriate method as a diagnostic technique. Neutrophil cells was collected from cow, horse and human bloods. These neutrophils were stimulated by phorbol myristate acetate. The reactive oxygens which were generated by this stimulation were detected by the ESR-ST method. 2, 2, 5, 5-Dimethylpyrroline N-oxide (DMPO) was used as a spin trapping reagent. Chemiluminescence spectroscopy was also employed as another method to detect the generation of reactive oxygens.
When the generation of reactive oxygens from human neutrophil cells was examined by the ESR-ST method, it was confirmed that the reactive oxygen which was generated from this cell was only superoxide (O_2^-). When this method was applied to the neutrophils of horse and cow, it was observed that the amount of (O_2^-) generated from horse neutrophils was the same as that of human netrophils, but that of cow was a twentieth less than those of human and horse neutrophils. This means that although the ESR-ST method is an excellent method to identify species of the generated reactive oxygens and to measure the amount of them in human and horse neutrophils, but the use of an optical method to observe chemilumineescence in addition to the ESR-ST method was necessary to quantitatively measure the amount of (O_2^-)in the case of neutrophils from cow.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Wakako Hiraoka: "Freeーradical reaction induced by OHーradical attack on cytosineーrelated compounds" Nucleic Acids Research. 18. 1217-1223 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋 恒夫: "凍結および浸透圧ストレスにおけるフリ-ラジカル産生:ヒト多形核白血球をモデルとして" 凍結および乾燥研究会誌. 36. 28-35 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平岡 和佳子: "PLD修復阻害剤ーピリミジン核酸誘導体の効果" 医学のあゆみ. 155. 377 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wakako Hiraoka: "Effects of 3´ーdeoxyadenosine (cordycepin) on the repair of Xーrayーinduced DNA singleー and doubleーstrand breaks in Chinese hamster V79 cells" Journal of Radiation Research. 31. 156-161 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wakako Hiraoka: "Characterization of free radicals in γーirradiated polycrystalline uridine 5´ーmonophosphate:a study combining ESR, spin trapping and HPLC" International Journal of Radiation Biology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikinori Kuwabara: "Chemical processes induced in nucleic acids by OH attack" Radiation Physics and Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraoka, W., Kuwabara, M. & Sato, F.: "Free-radical reactions induced by OH-radical attack on cytosine-related compounds : a study by a method combining ESR, spin trapping and HPLC" Nucleic Acids Research. 18. 1217-1223 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, T., Kuwabara, K., Hiraoka, W., Hosoda, M., & Sekiguchi, S.: "Generation of free radicals in human polymorphonuclear leukocytes by hyperosmotic stress and freeze-thaw cycle" Japanese Journal of Freezing and Drying. 36. 28-35 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraoka, W., Kuwabara, M. & Sato, F.: "Effects of 3'-deoxyadenosine (cordycepin) on the repair of X-roy-induced DNA single- and double-strand breaks in Chinese hamster V79 cells" Journal of Radiation Research. 31. 156-161 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraoka, W., Kuwabara, M. & Sato, F.: "Characterization of free radicals in gamma-irradiated polycrystalline uridine 5'-monophosphate : a study of combining ESR, spin trapping and HPLC" International Journal Radiation Biology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuwabara, M.: "Chemical processes induced in nucleic acids by OH attack" Radiation Physics and Chemistry.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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