1989 Fiscal Year Final Research Report Summary
A Study of Functional Abnormality by Environmental Contaminants.
| Project/Area Number |
01560339
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
NISHIMURA Masakazu UNIVERSITY OF OSAKA PREFECTURE, DEPARTMENT OF VETERINARY SCIENCE, ASSOCIATE PROFESSOR., 農学部, 助教授 (50011995)
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| Co-Investigator(Kenkyū-buntansha) |
KISO Yasuo UNIVERSITY OF OSAKA PREFECTURE, DEPARTMENT OF VETERINARY SCIENCE, ASSISTANT PROF, 農学部, 助手 (10142374)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Iprodione / Developmental toxicity / Neuronal Development / Functional malformation |
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| Research Abstract |
The effects of daily injection of iprodione to pregnant mice on neuronal functions of the new born mice were investigated. Iprodione (IPRD, Wako Pure Chemicals, Osaka) was dissolved in dimethyl sulfoxide (DMSO, Wako Pure Chemicals, Osaka) at a concentration of 100 mg/ml. Daily injection of IPRD (100 mg/kg) to the pregnant mice was started day 0 on gestation (Group 1)or 15th day on gestation (Group 2) and continued through delivery. Control pregnant mice was given vehicle on day o through delivery. New born mice from Group 2 had no behavioral sighns significantly different from the control neonates. However, both sexes of mice born from Group I showed body-weight-gain reduced significantly (P<0.05). These mice at 4 weeks old showed abnormally higher reactivity (P<0.05), Spontaneous activity (P<0.05) and touch response (P<0.05). The resting membrane potentials, frequency (F) of miniature end-plate potentials (m.e.p.ps) and the quantal content (m) of end-plate potentials (e.p.ps) were measured intracellularly at end-plates of diaphragm of male mouse in a high-Mg^<2+> bathing solution containing several concentrations of Ca^<2+>. The resting membrane potentials of end-plates were significantly decreased in 7 or 8 weeks old mice from Group 1. Also, in these mice, F was significantly (P<0.05) higher and m was significantly (P<0.05) larger than the controls, respectively. Slope of the regression line for 1n(m)-1n[Ca] relationship was approx. 3 in these mice, while control was 4. Thus, the treatment with IPRD of the mice for whole pregnant period may have a causal of abnormality of neuronal development.
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