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1990 Fiscal Year Final Research Report Summary

Histocytochemical Analysis for New Vasoactive Substances in CNS

Research Project

Project/Area Number 01570030
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionShiga University of Medical Science

Principal Investigator

YAMADA Hisao  Shiga University of Medical Science, Department of Anatomy, Associate Professor, 医学部, 助教授 (00142373)

Co-Investigator(Kenkyū-buntansha) KUMAMOTO Kenzo  Meiji College of Oriental Medicine, Department of Anatomy, Associate Professor, 鍼灸学部, 助教授 (20141509)
Project Period (FY) 1989 – 1990
KeywordsEndogenous digitalis-like substance (EDLS) / ANP / BNP / Endothelin / Vasoactive substance / Natriuresis / Hypothalamus / Neurohormone
Research Abstract

Recently, some humoral agents which play a role in water-balance, electrolyte metabolism and blood pressure control, i. e. ANP/BNP/CNP family (ANPs), endothelin (ET) and endogenous digitalis-like substance (EDLS) were identified and their chemical stucture determined. In this research, we analyzed these vasoactive substances with a histocytochemical technique using the central nervous system of rat, pig, dog and macaque.
The nerve terminals of ANP- and BNP-neurons were observed in the posterior horn of the spinal cords of rats and pigs. The BNP-neurons were seen in the pig hypothalamus, but were not found in the rat brain except the spinal cord. These results were confirmed by biochemical and physiological reports, which proposed that BNP existed mainly in the mammalian heart but not in the brain ; and CNP, a new peptide of ANPs is found in mammalian brain.
ET-immunoreactivities were not recognized in the mammalian central nervous system. The central nervous tissues have 10^2pg/g of ET, … More which level of bioactive substance cannot be detected with immunohistochemistry, though there were some immunohistochemical reports using the mammalian brain. In those reports, the researcher used the antibody, the epitope which had high homology in nelation to other bioactive substances. We made a ontogenetical study on this neuropeptide, i. e. ET in the rat with immunohistochemical and molecular biological technique. ET was first detected on day 19 of gestation in a salivary gland. Because ET is also considered to be a cell-proliferation factor, ET is possible to be the secondary discovered NGF (nerve growth factor).
EDLS were histochemically studied using anti-digoxin and anti-ouabain antibodies which have highーaffinity to EDLS. EDLS-neurons were distributed in the magnocellular nuclei of mammalian hypothalamus. Recent biochemical study shows EDLS resembles to ouabain, cis-trans-cis type steroid of plant origin. It is thought that cis-trans-cis type steroid exists in the mammalian brain as a neurohormone. Less

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Yamada H: "Immunohistochemical localization of atrial natriuretic polypeptide (ANP) in human atrial and ventricular myocardiocytes." Histochemistry. 89. 411-413 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] 山田 久夫: "食塩による昇圧と内因性ジギタリス様物質(EDLS)含有視床下部ニュ-ロン。" 自律神経. 25. 375-379 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yamada H: "Distribution of growth hormone releasing hormone (GRH)ーcontaining neurons in rat, macaque and human hypothalamus." Biomed Res. 10. 127-134 (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yamada H: "Histological study on ouabainーimmunoreactivities in the mammalian hypothalamus." Neurosci Lett,. (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yamada H: "Histochemical demonstration of the endogenous digitalisーlike substance (EDLS)ーcontaining neurons in the dog and macaque hypothalamus, using digoxinーantibody." Brain Res.

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      「研究成果報告書概要(和文)」より
  • [Publications] Katsura A: "Endothelin: Preparation of specific antibody, its application to immunohistochemistry and gene expression in salivary gland." J Histochem Cytochem.

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      「研究成果報告書概要(和文)」より
  • [Publications] Sako H: "Double immunocytochemical staining metod. In: Reviews on immunoassay technology (ed:Pal SB)Vol.3" Macmillan Press (Ulm, FRG), 173 (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kawata M: "Morphological aspects of atrial natriuretic peptideーimmunoreactive cells in the rat brain and heart. In: Proceeding of Kyoto Symposium on ANP(ed: Imura H)" Kodansha(Tokyo), (1990)

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      「研究成果報告書概要(和文)」より

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Published: 1993-08-12  

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