• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1990 Fiscal Year Final Research Report Summary

Extracellular Matrix Substances in the Development of the Nervous System

Research Project

Project/Area Number 01570033
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionJuntendo University School of Medicine

Principal Investigator

NISHIZUKA Masako  Juntendo school of Assistant University Medicine professor, 医学部, 講師 (20133332)

Project Period (FY) 1989 – 1990
KeywordsExtracellular matrix / Proteoglycan / Cerebral cortex / Chondroitin sulfate
Research Abstract

Proteoglycan molecules generally consist of core protein (s), oligosacharide chains and glycosaminoglycan chains. The glycosaminoglycan components in the brain were extracted from the cerebral tissues of postnatal rats at 1, 10, 20, 30 days and 3-4 months of age. Two dimensional electrophoresis revealed that the content of chondroitin sulfate increased in early postnatal days, Chondroitin sulfate was the major component at 10 days of age and thereafter. Considerable amounts of chondroitin sulfate were soluble in a phosphate buffered saline. While the amount of heparan sulfate was very low. The extractability of heparan sulfate was below the detectable level. Based on these findings, it was concluded that the major glycosaminoglycan in the cerebral tissues was the soluble chondroitin sulfate during and after the development. Chondroitin sulfate proteoglycans soluble in phosphate buffered saline were purified from the cerebral tissues of postnatal rats. Six core proteins were obtained from purified fraction by digestion with protease-free chondroitinase ABC. Their molecular weights by SDS-PAGE were 250, 220, 150, 130, 105, and 93KDa. The composition of these core proteins changes during development. The 150kDa component was the major core protein in adulthood. Its content was very low in 1-day-old rats, but increased rapidly by 10 days of age. Considerable amounts of the 250kDa and 130kDa components were present in immature and adult brains. However, the 150kDa or 93kDa appeared after day 20. In contrast, the 220kDa core protein was predominant at 1 and 10 days of age, and it accounted for more than 60% of the total core proteins in 1-day-old rats. The amount of the 220kDa component markedly decreased and it was below the detectable level on day 30 and thereafter. These rnsults suggest that different kinds of soluble chondroitin sulfate proteoglycans are involved in development of the brain.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] M. Nishizuka: "Dynamics of chondroitin sulfate proteoglycans in the brains of postnatal rats" Neuroscience Research.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Nishizuka: "Soluble chondroitin sulfate proteoglycans in the rat bran"

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Nishizuka: "Analyses of glycosaminoglycans in the central nervous system of postnatal rats"

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Nishizuka: "Neuronal structure and male female difference in the medial amygdala of rats" Biomedical Research. 10 S3. 323-327 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Nishizuka: "Ultrastructural characterization of prolactinーlike immunoーreactivity in rat medial basal hypothalamus" Neuroendocrinology. 51. 249-254 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Nishizuka: "Modification of nerve growth factor and sex steroids in development of hypothalamic neurons" Endocrinologia Experimentalis. 24. 77-88 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishizuka, M. and D. W. Pfaff: "Intrinsic synapses in the ventromedial nucleus of the hypothalamus : An ultrastructural study" Journal of Comparative Neurology. 286. 260-268 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nushizuka, M. and D. W. Pfaff: "Ultrastructural characterization of prolactinlike immunoreactivity in rat medial basal hypothalamus" Neuroendocrinology. 51. 249-254 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizuka, M. and Y. Arai: "Effects of nerve growth factor on development of dopamine neurons in culture or transplantation into the third or lateral ventricle" Neurosciene Research. S9. 55 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizuka, M. and D. W. Pfaff: "Medial preoptic islands in the rat brain ; electron microscopic evidence for intrinsic synapses" Experimental Brain Research. 77. 295-301 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizuka, M. and Y. Arai: "Modification of nerve growth factor and sex steroids in development of hypothalamic neurons" Endocrinologia Experimentalis. 24. 77-86 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizuuka, M., S. Murakami and Y. Arai: "Neuronal structure and male female difference in the medial amygdala of rats" Biomedical Research. 10, S3. 323-327 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohta, H., M. Nishizuka, Y. Arai and H. Saito: "Effects of nerve growth factor (NGF) on survival of superior cervical ganglion (SCG) transplanted into the third ventricle" Japanese Journal of Pharmacology. 53. 11-18 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Eto, K. M. Nishizuka, R. Iizuka, and Y. Arai: "Histochemical study of fetal cholinergic neurons transplanted into the brain of adult rats" Juntendo Medical Journal. 36.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizuka, M., A. Oohira and Y. Arai: "Dynamics of chondroin sulfate proteglycans in the brain of postnatal rats" Neuroscience Research. S13.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishzizuka, M., A. Oohira and Y. Arai: "Soluble chondroitin sulfate proteoglycans in the rat brain"

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizuka, M., A. Oohira and Y. Arai: "Analyses of glycosaminoglycans in the central nervous system of postnatal rats"

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-08-12  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi