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1990 Fiscal Year Final Research Report Summary

Electrophysiological Studies on the Respiratory Rhythm Generation.

Research Project

Project/Area Number 01570061
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SUZUE Toshihiko  Tokyo Med. & Dent. Univ. Assist. prof., 医学部, 助手 (40143565)

Project Period (FY) 1989 – 1990
Keywordsrespiration / rat / brainstem / rhythm
Research Abstract

Electorophysiological nature of the respiratory rhythm generation were studied using in vitro rat brainstem-spinal cord preparation from newborn rats. Rhythm generation-related neurons were identified in rostral ventrolateral medulla by analyzing the reset of the rhythm under various calcium concentrations. It was found that the experimental condition of calcium 0.2 mM and magnesium 5 mM, a condition that has been employed for blocking the synaptic transmission of the in vitro CNS ( Central Nervous System ) preparation, was not sufficient for the complete block of the synaptic transmission. Under this condition the intracellularly recorded synaptic potentials evoked by a single shock stimulations to afferent neurons were decreased, but large synaptic potentials could still be evoked after repetitive stimulations of afferent neurons, and even action potentials could often be evoked in thepostsynaptic neurons. Therefore, it is possible that rhythmic activities of neurons recorded under this condition may involve synaptic activities of neurons, and the presence of rhythm cannot be a conclusive evidence that pacemaker cells exist in the respiratory rhythm generation systems. The effects on the rhythm of antagonists of glutamate, a major excitatory transmitter in the CNS, was also studied. When glutamte antagonists, CNQX, APV or kynurenic acid, were applied, they dramatically decreased the rate of the rhythm. This is rather unexpected if the simple pacemaker hypothesis for the rhythmgeneration holds true. In conclusion, the obtained results suggest that the simplest hypothesis for the respiratory rhythm generation, a pacemaker cell hypothesis, has to be critically re-evaluated and a possible active involvement of excitatory transmitters in the rhythm generation has to be reconsidered.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Suzue,T.: "An in vitro transplacental perfusion method for the maintenance of physiological activities of late gestation rodent fetuses." Neuroscience Research. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzue,T.: "Rat fetus in late gestation in vitro:Its physiology and pharmacology." Japanese J. Pharmacology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzue,T.: "A monoclonal antibody that define rostrocaudal position in the mammalian nervous system." Neuron. 5. 421-431 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzue,T: "Rat fetus in late gestation can be kept in vitro." Proceedings of the Japan Academy.66B. 177-181 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzue,T.: "Physiological activities of rat and mouse fetus maintained in vitro." Japanese Journal of Physiology. 40. S216- (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzue,T.: "発生神経科学のトレンド." 生体の科学.40. 153-155 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzue, T.: "An in Vitro Transplacental-perfusion method for the maintenance of physiological activities of late gestation rodent fetuses." Neuroscience Research. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzue, T.: "Rat fetus in late gestation in vitro : Its physiology and pharmacology." Japanese J. Pharmacology. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzue, T. & Kawabata, S.: "Staining of living fetal neurons with an in vitro transplacental-perfusion method for late gestation rat fetuses." Japanese Journal of Physiology. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzue, T., Kaprielian, Z. & Patterson, P. H.: "A monoclonal antibody that defines rostocaudal position in the mammalian nervous system." Neuron. Vol. 5. 421-431 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzue, T.: "Rat fetus in late gestaion can be kept in vitro." Proceedings of the Japan Academy,. Vol. 66B.177-188 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzue, T.: "Physiological activity of rat and mouse fetus maintained in vitro." Japanese Journal Physiology. Vol. 40. 216 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaprielian, Z. Suzue, T & Patterson, P. H.: "Biochemical characterization of the ROCAl antigen." Society for Neuroscience Abstracts. Vol. 16. 151 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzue, T., Imrich, J. & Patterson, P. H.: "Monoclonal antibodies that display rostocaudal difference in binding in the peripheral nerve tissue." Neuroscience Research. supple.9. 45 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzue, T.: "Trends in Developmental Neuroscience." Seitaino Kagaku. Vol. 40. 153-155 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzue, T., Imrich, J. & Patterson, P. H.: "Rostrocaudal immunohistochemical difference in the peripheral nervous system." Japanese Journal of Physiology. Vol. 39. 273 (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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