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1990 Fiscal Year Final Research Report Summary

Molecular Genetic Study of Mouse Senile Amyloidosis

Research Project

Project/Area Number 01570190
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKuoto University

Principal Investigator

HIGUCHI Keiichi  Chest Disease Research Institute, Kyoto University. Lecturer, 胸部疾患研究所 講師 (20173156)

Co-Investigator(Kenkyū-buntansha) HOSOKAWA Masanori  Chest Disease Research Institute, Kyoto University. Associate Professor, 胸部疾患研究所, 助教授 (00127135)
TAKEDA Toshio  Chest Disease Research Institute, Kyoto University. Professor, 胸部疾患研究所, 教授 (00027088)
Project Period (FY) 1989 – 1990
KeywordsSenile Amyloidosis / Mouse / apoA-II / Senescence Accelerated Mouse (SAM) / PCR / RFLP / Genetic Study / mRNA
Research Abstract

The apoA-II gene was amplified by polymerase chain reaction (PCR) from chromosomal DNA of nine inbred strains of mice. Sequence analysis of the PCR products indicated the presence of three types of apoA-II genes. Three types of apoA-II proteins (A, B and C) were predicted from the nucleotide sequence of apoA-II cDNA. Substitution of amino acid residues was noted at 4 positions. Each type was identifiable by digestion of PCR amplified apoA-II DNA, using restriction enzyme Cfr13I and MspI. The mouse strains, SAM-P/1, SAM-P/2, SJL/J, A/J had type A apoA-II. These strains generally exhibit amyloid deposition from relatively younger age. Examination of types of apoA-II and amyloid deposition in the F2 hybrid mice showed that apoA-II amyloid deposition was present only in the homozygous mice for type A apoA-II. We postulate that the molecular structure of apoA-II may be an important factor involved in the development of senile amyloidosis in mice.
The expression levels of apoA-II mRNA in the liver of the SAM-P/1 strains of mice decreased rapidly with advancing age. The expression levels of apoA-II mRNA at the age of 14 months was 50 % of the level at the age of 2 months.
The new system was developed for determination of amyloid fibrils specifically and kinetics of amyloid fibril polymerization was analyzed.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 米津 智徳: "Structural organization of the senile amyloid protein gene in a strain of Senescence Accelated Mouse (SAM)." Gene. 84. 187-191 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内木 宏延: "Fluometric examination of tissue amyloid fibrils in murine senile amyloidosis; use of the fluorescent indicator Thioflavine T." Laboratory Investigation. 62. 768-773 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内木 宏延: "Kinetic analysis of amyloid fibril polymerization invitro." Laboratory Investigation. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内木 宏延: "Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye,Thyoflavine T." Analytical Biochemistry. 177. 244-249 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉岡 秀幸: "Immunohistochemical examination of Peyer´s patches in Senescence Accelerated mice." Autoimmunity. 8. 25-35 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 樋口 京一: "動物のアミロイドーシス(SAMにおける老化アミロイドーシスを中心として)" 基礎老化研究. 14. 93-94 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 樋口 京一: "Amyloid and Amyloidosis 1990 (Molecular genetic study of mouse senile amyloidosis)" Kluwer Academic Publishers, 600(4) (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 樋口 京一: "老化とアミロイドーシス(内科MOOK No35ーアミロイドーシス)" 金原出版株式会社, 400(9) (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] YONEZU Tomonori: "Structural organization of the senine amyloid gene in a strain of Senescence Accelerated Mouse (SAM)" Gene. 84. 187-191 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAIKI Hirinobu: "Fluorometric examination of tisssue amyloid fibrils in murine senile amyloidosis ; use of the fluorescenct indicator Thioflavine T." Laboratory Investigation. 62. 768-773 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAIKI Hironobu: "Kinetic analysis of amyloid fibril polymerization in vitro" Laboratory Investigation. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAIKI Hironobu: "Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye Thyoflavine" Analytical Biochemistry. 177. 244-249 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] YOSHIOKA Hideyuki: "Immunochemical examination of Peyer's patches in senescence accelerated mice." Autoimmunity. 8. 25-35 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] YOSHIOKA Hideyuki: "Autoimmune abnormalities in a murine model of accelerated senescence." Clinical and Experimental Immunology. 75. 129-135 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIGUCHI Keiichi: "Molecular genetic study of mouse senile amyloidosis." Amyloid and Amyloidosis. (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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