1990 Fiscal Year Final Research Report Summary
Analysis of the Pathogenic Mechanism in Schistosomiasis-Functional Decline of the Mitochondria in Parenchymal Liver Cells
| Project/Area Number |
01570221
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Department of Parasitoloby, School of Medicine, Keio University |
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Principal Investigator |
TANABE Masanobu Keio University, School of Medicine Department of Parasitology Assistant professor, 医学部, 専任講師 (80051928)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Nobuaki Keio University, School of Medicine Department of Parasitology Research associat, 医学部, 助手 (50177523)
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| Project Period (FY) |
1989 – 1991
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| Keywords | Schistosoma mansoni / Hepatocyte / Mitochondria / Respiration / Mg<D1++@>D1-ATPase / Egg-granuloma / Free proline |
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| Research Abstract |
A mitochondria-rich fracion isolated from the livers of ICR and C3H female mice 8 to 10 weeks after Schistosoma mansoni infection showed to be uncoupling state of the succcinateーsupported respiration. A moderate to strong mitochondrial Mg^<++>-dependent ATPase activity at neutral pH was demonstrated cytochmecally in the parenchymal liver cells of S. mansoni-infected mice. Moreover, the enzymatic activities of malate dehydrogenase, NADH dehydrogenase, cytochrome oxidase and glutamate dehydrogenase also decreased significantly in the mitochondria-rich fraction from the livers of infected mice. Furthermore, an ultrastructural observation of the liver of infected mice clearly demonstrated some morphological changes of hepatic mitochondria, i. e., swelling, disappearance of cristae and deciline of electron density of matrix.
The uncoupling state of the respiration and the ultrastructural changes of the hepatic mitochondria were also demonstrated in the uninfected, normal mice treated with in
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traperitoneal injection of the dialysate of 15,000g x 30 min supernatant of crude extracts of egg-granulomas. However, there was no appreciable change in the respiration of hepatic mitochondria in the mice treated with culture fluid or crude extract of S. mansoni adult worms. The active substance was partially purified from crude extract of egg-granulomas by ammonium sulfate fractionation, Sephadex G-200 gel filtration and DEAEーsephadex A-25 column chromatography. Intraperitoneal injection of the partially purified substance into unifected, normal mice resulted in the decline of the activities of several enzymes in hepatic metochondria and in the ultrastructural changes in the mitochondria of parencymal liver cells. The same biochemical changes were also observed in the primary cultured hepatocytes treated with the partially purified substance.
These findings suggest that the egg-granulomas produce some substance(s) which may be responsible for reduction of the activities of respiration and several enzymes localized in the mitochondria of the parenchymal liver cells in S. mansoni-infected mice. Less
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[Publications] Tanabe, M., Sekiguchi, T., Kaneko, N., Kobayashi, S., Takeuchi, T., et al.: "Elevation of laminin and betaーSubunit of prolyl 4ーhydroxylase in the sera of human subjects with schistosomiasis mansoni." Japanese Journal of Experimental Medicine. 59. 109-119 (1989)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tanabe, M., Okazaki, M., Okazaki, M., Kobayashi, S., Kaneko, N., et al.: "Serological studies on schistosomiasis mansoni in the northeast Brazil (1)." Revista Institute Medicina tropical Sao Paulo. 32. 121-131 (1990)
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「研究成果報告書概要(和文)」より
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[Publications] Concalves, J., Tanabe, M., Medeiros, F., Goncalves, F., Aca, I., et al.: "Parasitological and serological studies on amoebiasis and other intestinal parasitic infections in the rural sector around Recife,northeast Brazil." Revista Institute Medicina tropical Sao Paulo. 32. 428-435 (1990)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tababe, M., Sekiguchi, T., Kaneko, N., Kobayashi, S., Takeuchi, T., Coutinho, A., Tateno, S., Maryuama, K., and Okazaki, I.: "Elevation of laminin and beta-subunit of prolyl 4-hydoxylase in the sera of human subjects with schistosomiasis mansoni." Japanese Journal of Experimental Medicine. 59. 109-119 (1989)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tanabe, M., Okazaki, M., Okazaki, M., Kobayashi, S., Kaneko, N., Sekiguchi, T., Tateno, S., Motta, S., and Takeuchi, T.: "Serological studies on schistosomiasis mansoni in the northeast Brazil (I)." Revista Institute Medicina Tropical Sao Paulo. 32. 121-131 (1990)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Goncalves, J., Tanabe, M., Medeiros, F., Goncalves, F., Aca, I., Motta, S., Tateno, S., and Takeuchi, T.: Revista Institute Medicina Tropical Sao Paulo. 32. 428-435 (1990)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Miyahira, Y., Takeuchi, T., Okuzawa, E., Motta, S., Tanabe, M., and Miura, S.: "Pathogenic Entamoeba histolytica infection in a steady couple of Japanese homosexual men." Japanese Journal of Parasitology. 39. 497-499 (1990)
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「研究成果報告書概要(欧文)」より
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[Publications] Tanabe, M., Miyahira, Y., Okuzawa, E., Sagawa, M., Takeuchi, T., and Shinbo, T.: "A case report of extopic anisakiasis." Japanese Journal of Parasitology. 39. 397-399 (1990)
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「研究成果報告書概要(欧文)」より
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