• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1990 Fiscal Year Final Research Report Summary

Studies on the effects of ADP-ribosyltransferase activities of staphylococcal cytolytic toxins on the signal transduction in cell membrane.

Research Project

Project/Area Number 01570228
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionChiba University

Principal Investigator

NODA Masatoshi  Chiba University, School of Medicine, Professor, 医学部, 教授 (60164703)

Co-Investigator(Kenkyū-buntansha) MORINAGA Naoko  Chiba University, School of Medicine, Assistant, 医学部, 助手 (20092108)
KATO Iwao  Chiba University, School of Medicine, Professor, 医学部, 教授 (40012702)
Project Period (FY) 1989 – 1990
KeywordsStaphylococcal alpha-toxin / Leukocidin S and F comp. / ADP-ribosyltransferase / Phospholipase A_2 / Phospholipase C / GTP-binding protein / Protein kinase C / PI代謝
Research Abstract

1. Staphylococcal alpha-toxin resulted in ADP-ribosylation of the 37 and 41 kDa proteins of a membrane preparation from rabbit erythrocytes. In the presence of 100 muM GTP, the toxin ADP-ribosylated proteins of 54 and 59 kDa and potentiated ADP-ribosylation of the 37 and 41 kDa forms. GTP had no effect on ADP-ribosylation of membrane proteins in the absence of alpha-toxin.
2. Incubation of a membrane preparation of rabbit polymorphonuclear leukocytes with the S and F components of staphylococcal leukocidin resulted in ADP-ribosylation of the 37 and 41 kDa proteins, respectively. Furthermore, the 37, 41, 54 and 59 kDa proteins were ADP-ribosylated by leukocidin in the presence of GTP.
3. The ADP-ribosylation of these proteins was observed to be dependent on the incubation time and toxin dose and was abolished by prior boiling. Addition of agmatine did not attenuate ADP-ribosylation of these proteins.
4. The S component of staphylococcal leukocidin ADP-ribosylated 37 kDa GTP-binding protein involved in the transduction to activate phospholipase A_<2'> and the F component resulted in ADP-ribosylation of 41 kDa GTP-binding protein involved in the transduction to activate phosphoinositide-specific phospholipase C.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Xia Wang,Masatoshi Noda and Iwao Kato: "Stimulatory effect of staphylococal leukocidin on phosphoinositide metabolism in rabbit polymorphonuclear leukocytes" Infection and Immunity. 58. 2745-2749 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 越部 融、野田 公俊、加藤 巌: "黄色ブドウ球菌α毒素によるphosholipase A_2 と cyclooxigenaseの活性化機構" 臨床と徴生物. 17. 219-220 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 王 夏、野田 公俊、加藤 巌: "黄色ブドウ球菌ロイコシジンのPI kinaseに及ぼす影響" 臨床と徴生物. 17. 220-220 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野田 公俊: "細菌由来蛋白質毒素によるADPーリボルシ化作用" 臨床検査. 34. 1661-1663 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 越部 融、加藤 巌、野田 公俊、高橋 英世: "黄色ブドウ球菌α毒素のホスホリパ-ゼA_2およびシクロオキシゲナ-ゼ活性に及ぼす影響" 医学と生物学. 122. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakao Morinaga,Masatoshi Noda and Iwao Kato: "Different effects of staphylococcal leukocidin on ionsitol phosphlipid metabolism in HLー60 cells and differentiated HLー60 cells by dimethyl sulfoxide." Life Science Advances. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 宇井 理生、高井 義美、堅田 利明 編、野田 公俊、加藤 巌: "羊土社(東京)" GTP結合蛋白質と情報伝達,

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato, I., Noda, M.: "ADP-ribosylation of cell membrane proteins by staphylococcal alpha-toxin and leukocidin in rabbit erythrocytes and polymorphonuclear leukocytes." FEBS Letter. 225. 59-62 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wang, X., Noda, M., Kato, I.: "Stimulatory effect of staphylococcal leukocidin on phosphoinositide metabolism in rabbit polymorphonuclear leukocytes." Infect. Immun.58. 2745-2749 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noda, M.: "ADP-ribosylation by bacterial toxins." J. Medical Technology. 34. 1661-1663 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koshibe, T., Kato, I., Noda, M., Takahashi, H.: "Effects of staphylococcal alpha-toxin on phospholipase A_2 and cyclooxigenase in rabbit polymorphonuclear leukocytes." Medicine and Biology. 122. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noda, M., Kato, I.: "GTP-binding proteins and signal transduction." Experimental Medicine. vol. 8. 260-264 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-08-12  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi