1991 Fiscal Year Final Research Report Summary
Transcriptional Regulation of Major Histocompatibility Complex Class I antigen gene and oncogenesis and Transplantaion.
| Project/Area Number |
01570279
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | RIKEN (The Institute of Physical and Chemical Research) |
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Principal Investigator |
YOKOYAMA Kazushige RIKEN, Gene Bank, Research Scientist., ジーンバンク室, 研究員 (80182707)
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| Project Period (FY) |
1989 – 1991
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| Keywords | MHC Class I gene / Transcriptional Regulation / Adenovirus E1A / Nuclear Factor / CAA repeat / TFIID / Domain analysis / Rb protein |
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| Research Abstract |
We have investigated the transcriptional regulation of MHC Class I gene to identify the controlled elements in the MHC Class I gene promoter. To understand this molecular regulation is the first step to know the immunological regulation of "Self-Nonself" discrimination. We focus upon the controlled regulation of Class I MHC gene expression by adenovirus type 12 EIA. First, we determined the nucleotide sequence of the promoter region of MHC Class I H-2K^<bml> gene to compare the parent H-2K^b gene promoter. To identify the cis-element in H-2K^b gene, a seubs of deletion CAT mutants of the promoter region were constructed and used for the analysis of the CAT activity. Thus, we have identified the positive and negative elements in the H-2K^b promoter which were corresponded with the ElA-mediated response. By using a gel-shift and the footprint studies, we have characterized the nuclear proteins to bind Co this sequence. We also obtained the gene corresponding to bind to these elements, CAA repeated. motif, of the H-2K^b gene. These gene products including TFIID were responsible to the cooperative interaction with the EIA and Rb proteins to perform the negative regulation of H-2 Class I gene by ElA.
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Research Products
(26 results)
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[Publications] Tashiro, H., Ozawa, K., Tang, X., Nakai, H., Eki, T., Murakami, Y., Soeda, E. and Yokoyama, K.: "Single DNA marker generated by "YAC-Alu PCR" that is end-specific." Jpn. J. Human Genet.36. 229-243 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shin, E-K., Matsuda, F., Nagaoka, H., Fukita, Y., Imai, T. yokoyama, K., Soeda, E and Honjo, H.: "Physical map of the 3'region of the human immunoglobulin heavy chain locus : clustering of autoantibody-related variable segments in one haplogype." The EMBO. J.10. 3641-3645 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Imai, T., Yokoyama, K, Ozawa, K., Tashiro, H., Miwa, T., Umehara, Y, Iida, A., Tang, X., Murakami, Y., Eki, T. and Soeda, E.: "Collection of YAC clones derived from human chromosome 21." Cold Spring Harbor Meeting. "Genome Mapping & Sequencing, " 102, CSH, USA, MAY. (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ozawa, K., Tang, X., Hou, D., Eki, T., Murakami, Y., Yokoyama, K. and Soeda, E.: "Generation of STSs for physical mapping of human chromosomes-application to the mapping of human chromosome 21 using YACs." Human Genome III 30, San Diego, USA. (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Honjo, T., Matusda, F., Shin, E. K., Nagaoka, H., Takahashi, S., Imai, T., Yokoyama, K., and Soeda, E.: "Structural analysis of the human immunoglobulin heavy chain locus using yeast artificial chromosome system" The 11th Int. Workshop on human genome mapping. (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Soeda, E., Imai, T., Ozawa, K., Tashiro, H., Miwa, T., Umehara, Y., Iida, A., Xiaoren, T., Eki, T., Murakami, Y. and Yokoyama. K.: "YAC clones anchored at human chromosome 21 and linking." The 11th Int. Workshop on human genome mapping. (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Eki, T., Yokoyama, K., Tashiro, H., Ozawa, K. Murakami, Y., Watkins, P. C. and Soeda, E.: "Yeast artificial chromosome clones and STS markers anchored at human chromosome 21." Jpn. J. Human Genet.36. 331-334 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kitabashi, I., Kawakami, Z., Chiu, R., Ozawa, K., Matsuoka, T., Toyoshima, S., Umesono, K., Evans, R. M., Gachelin, G. and Yokoyama, K.: "Tranccriptional regulation of the c-jun gene by retinoic acid and E1A during differentiation of F9 cells." The EMBO. J.11. 167-175 (1992)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ozawa, K., Murakami, Y., Eki, T., Soeda, E. and Yokoyama, K.: "Mapping of the gene family for human heat shock protein 90a to chromosomes 1, 4, 11 and 14." Genomics. 12. 214-220 (1992)
Description
「研究成果報告書概要(欧文)」より
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