• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1990 Fiscal Year Final Research Report Summary

Molecular Biological Analysis of Effects of Ethanol on the Expression of Hepatic Plasma Membrane Receptor and Role in the Hepatic Disease.

Research Project

Project/Area Number 01570407
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionDepartment of Internal Medicine (Section 4), Sapporo Medical College

Principal Investigator

KOHGO Yutaka  Department of Internal Medicine (Section 4), Sapporo Medical College Associate Professor, 医学部, 助教授 (10133183)

Co-Investigator(Kenkyū-buntansha) MOGI Yoshihiro  Department of Internal Medicine (Section 4), Sapporo Medical College Assistant P, 医学部, 講師 (30190953)
NIITSU Yoshiro  Department of Internal Medicine (Section 4), Sapporo Medical College Professor, 医学部, 教授 (10045502)
Project Period (FY) 1989 – 1990
KeywordsEthanol / Asialoglycoprotein receptor / Protein synthesis regulation
Research Abstract

We studied the effect of ethanol on the expression of asialoglycoprotein receptor of a human hepatoma cell line, Hep G2 cells, which has been shown to maintain a large variety of normal hepatocyte functions. By ethanol treatment, the number of asialoglycoprotein receptors on the cell surface decreased to 60% of the control, with no change of binding affinity. The decrease of cell surface asialoglycoprotein receptors was parallel to that of total receptor number including surface and intracellular receptors. Internalization of asialoglycoprotein receptors was also diminlshed to 45% of control cells. Northern blot analysis by using a human asialoglycoprotein receptor oligonucleotide probe showed an increase of the specific asialoglycoprotein receptor mRNA in ethanol-treated cells. The amount of newly synthesized AGPR was decreased in ethanol-treated HeP G2 cells. This increase was reversible when cells were removed from ethanol. These results suggest that ethanol metabolite directly induce the decrease of asialoglycoprotein receptor in human hepatocytes at post translational level. The paradoxical increase of asialoglycoprotein receptor mRNA after ethanol treatment could be explained by the possible feed-back mechanism which controls asialoglycoprotein receptor gene transcription and/or impaired degradation of receptor mRNA.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 加藤 淳二 他: "エタノ-ルによるヒト肝asialoglycoprotein receptor発現異常の解析" アルコ-ル代謝と肝. 8. 135-140 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中谷 玲二 他: "アルコ-ル性肝障害における血中遊離型アシアロ糖蛋白レセプタ-測定の意義" アルコ-ル代謝と肝. 9. 118-121 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中谷 玲二 他: "各種サイトカイン(ILー1,ILー6,TNFー2)による肝アシアロ糖蛋白受容体の発現調節に及ぼすエタノ-ルの影響" アルコ-ル代謝と肝. 10. 168-172 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 茂木 良弘 他: "ヒト肝細胞培養株を用いたアシアロ糖蛋白受容体の発現に及ぼすエタノ-ルの影響" Gastroenterology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohgo Y,et al: "Targeted diagnosis and therapy of liver disease: CellーSurface receptors and liver directed agents." Wu G.Y.and Wu C.H., 15 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato, J., Kohgo, Y., Nakaya, R., Mogi, Y., Sasaki, K., Numata, T., Hukushi, A. and Niitsu, Y.: "Analysis of effects of ethanol on the expression of human hepatic asialoglycoprotein receptor." Alcohol Metabolism and the Liver. 8. 135-140 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakaya, R., Mogi, Y., Kato, J., Numata, T., Nakajima, M., Sasaki, K., Kohgo, Y., Niitsu, Y. and Ohta, K.: "Evaluation of serum asialoglycoprotein receptor in patients with alcoholic liver disease." Alcohol Metabolism and the Liver. 9. 118-121 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohgo, Y., Kondo, H., Mogi, Y. and Niitsu, Y.: "Mechanism and clinical significance of soluble hepatic cell surface receptors." Targeted diagnosis and therapy of liver disease : Cell-surface receptors and liver-directed agents.Marcel Dekker, Inc.305-319 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakaya, R., Kohgo, Y., Mogi, Y., Kato, J., Numata, T., Kumai, R., Nakajima, M. and Niitsu, Y.: "Effect of ethanol on the regulation of hepatic asialoglycoprotein receptor expression by cytokines (IL-1, IL-6 and TNF-alpha)" Alcohol Metabolism and the Liver. 10. 168-172 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mogi, Y., Kohgo, Y., Nakaya, R., Kato, J., Sasaki, K., Numata, T., Nakajima, M., Kumai, R., Wu, G. H. and Niitsu, Y.: "Direct effects of ethanol on the expression of asialoglycoprotein receptor in a human hepatoma cell line." Gastroenterology.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-08-12  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi