1991 Fiscal Year Final Research Report Summary
Study on the mechanism of pulmonary fibrosis in rheumatic diseases.
Project/Area Number |
01570433
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
Respiratory organ internal medicine
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Research Institution | Saga Medical School |
Principal Investigator |
OHTA Akihide Saga Med. Sch., Int. Med., Assist. Prof., 医学部, 講師 (40128129)
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Co-Investigator(Kenkyū-buntansha) |
MORITO Fumitaka Saga Med. Sch., Int. Med., Res. Edu. Assoc., 医学部, 教務員 (20157910)
MOTOMURA Mitsuaki Saga Med. Sch., Int. Med., Assist. Prof., 医学部, 助手 (10210098)
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Project Period (FY) |
1989 – 1990
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Keywords | Pulmonary fibrosis / Rheumatic diseases / Alveolar macrophage / Bronchoalveolar lavage / Collagen / Cytokines / Growth factors / Fibroblasts |
Research Abstract |
In order to elucidate the mechanism of pulmonary fibrosis in rheumatic diseases, we performed bronchoalveolar lavage (BAL) study in 13 cases of rheumatic diseases with pulmonary fibrosis, including 5 cases of rheumatoid arthritis, 2 cases of scleroderma, 2 cases of polymyositis, 3 cases of mixed connective tissue disease, and 1 case of systemic lupus erythematosus, and in 5 cases of lung cancer without pulmonary fibrosis as a control group. The supernatant of cultured alveolar macrophages obtained from BAL from 2 cases of rheumatoid arthritis, I case of scleroderma, 1 case of polymyositis and 3 controls had a stimulatory effect on the growth of human lung-derived fibroblast line, WI-38. Especially, the culture supernatant from 1 patient with polymyositis (pt-9) and I control (control-4) contained high stimulatory effect on the growth of WI-38. However, there were no differences in the stimulatory effect between the rheumatic disease group with pulmonary fibrosis and the control group without pulmonary fibrosis. -Then, the effect of culture supernatant of alveolar macrophages from pt-9 and control-4 on the collagen production by WI-38 cells was analyzed. As a result, both showed stimulatory effect on collagen production, and especially, culture supernatant from pt-9 contained higher stimulatory effect than that from control-4, suggesting that the factor(s) responsible for this effect might be associated with the fibrotic process. Furthermore, the level of TNF-alpha, IL-1beta, IL-6, and IFN-gamma in BAL supernatant was analyzed. There were no differences in the level of these cytokines between rheumatic disease group and control group. However, BAL supernatant from pt-9 showed the lowest IFN-gamma level. This suggested that the low level of IFN-gamma might be responsible for the fibrotic process because IFN-gamma was a potent inhibitor of collagen production, although another important factors such as PDGF and TGF-B must be examined further.
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