1990 Fiscal Year Final Research Report Summary
An Application of Growth Factor for the Maturation of Premature Infants
| Project/Area Number |
01570530
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Kobe University |
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Principal Investigator |
UETANI Yoshiyuki Kobe Univ., University Hospital, Research Associate, 医学部附属病院, 助手 (40168620)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Masafumi Kobe Univ., School of Medicine, Associate Professor, 医学部, 講師 (10157266)
SANO Kimihiko Kobe Univ., School of Medicine, Research Associate, 医学部, 助手 (40205993)
NAKAMURA Hajime Kobe Univ., School of Medicine, Professor, 医学部, 教授 (40030978)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Premature infant / Growth factor / myo-Inositol |
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| Research Abstract |
Myo-inositol is a constituent of phosphatidyl-inositol which is known as a second messenger of growth factor. Recently many attentions have been paid for the action of growth factor of myo-inositol. In premature babies who need many growth factors for their growth, the actions of those growth factors should be well organized.
Our study is aimed to clarify the growth factor action of myo-inositol in premature baby. We measured myo-inositol in amniotic fluid and serum of the neonates by gas-chromatography. The concentrations of myo-inositol in both amniotic fluid and neonatal serum were reversely correlated with the gestation. al weeks. And very low birth weight neonates showed the highest concentration of Myo-inositol in the serum. These results suggested myo-inositol might have some function for the normal growth of very low birth weight infants.
To determine the effect for gene expression of myo-inositol, we employed a reverse-transcriptase polymerase Chain Reaction (PCR) to determine messenger RNA levels in cell. To confirm this method, we analyzed messenger RNA of Duchenne Muscular Dystrophy (DMD) patient. The messenger RNA was well reverse-transcribed and amplified by PCR. In one patient with DMD who has a very small deletion within exon 19, we analyzed messenger RNA sequence and found that the exon with the deletion was skipped during maturation of messenger RNA. This result showed not only our method was useful but also the maturation of messenger RNA is controlled by exon sequence.
Since we could establish the method for analysis of messenger RNA, we will further analyze the mechanism of growth factor action of myo-inositol by using this method.
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Research Products
(8 results)
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[Publications] Matsuo, M., Masumura, T., Nakajima, T., Kitoh, Y. Takumi, T., Nishino, H., Koga, J. and Nakamura, H.: "A very small frame-shifting deletion within exon 19 of the Duchenne muscular dystrophy gene." Biochem Biophys Res Commun. 170. 963-967 (1990)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakajima, T., Matsuo, M., Kitoh, Y., Nishio, H., Takumi, T., Koga, J. and Nakamura, H.: "Screening of gene deletion in Japanese patients with Duchenne muscular dystrophy by polymerase chain reaction." J Neurol.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsuo, M., Masumura, T., Nishio, H., Nakajima, T., Kitoh, Y., Takumi, T., Koga, J. and Nakamura, H.: "Exon skipping during splicing of dystrophin mRNA precursor due to an intra-exon deletion in the dystrophin gene of Duchenne muscular dyatrophy Kobe." J Clin Invest.
Description
「研究成果報告書概要(欧文)」より
