| Co-Investigator(Kenkyū-buntansha) |
KAWADA Masaaki Pediatric Cardiovascular Surgery, Research Associate, 医学部, 助手 (30177703)
KUROSAWA Hiromi Pediatric Cardiovascular Surgery, Assistant Professor, 医学部, 助教授 (50075511)
IMAI Yasuharu Pediatric Cardiovascular Surgery, Professor, 医学部, 教授 (30075246)
IMAMURA Shin-ichiro Research Division, Research Associate, 医学部, 助手 (00176497)
TAKAO Atsuyoshi Pediatric Cardiology, Professor Emeritus, 医学部, 名誉教授 (70075167)
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| Research Abstract |
Changes in the relative amounts of three cardiac myosin heavy chain (MHC) isozymes V1 : alpha alpha homodimer, V2 : alpha beta heterodimer, and V3 : beta beta homodimer are believed to be responsible, at least in part, for the altered cardiac performance. We have been shown that the distribution of cardiac MHC mRNA expression and isozymes is modified in developmental change, hemodynamic overload, hormonal stimuli and with some drugs. Also, previous studies have demonstrated that the MHC isozyme transition during developmental change, hemodynamic overload and hormonal stimuli is mainly regulateretranslational mechanisms. Based on these results, the aim of this study is to define the methodology of human cardiac MHC gene expression and isozyme distribution using S1 nucrease mapping analysis and pyrophosphate acrylamide gel electrophoresis, respectively, in order to clarify the etiology of disease and early diagnosis. S1 nucrease mapping analysis has been performed using human beta cardiac MHC oligonucleotide as a probe. And also, we succeeded to separate human cardiac MHC isozyme using different composition of pyrophosphate acrylamide gel on very small amount of operative sample (5mg). We think that S1 nucrease mapping analysis and pyrophosphate acrylamide gel electrophoresis are very powerful methods to clarify the etiology of disease and early diagnosis.
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