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1991 Fiscal Year Final Research Report Summary

Morphological and Functional Plasticity in the Central Noradrenergic Neurons of the Stress-induced Depression Model Rats

Research Project

Project/Area Number 01570603
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionMie University

Principal Investigator

NOMURA Junichi  Mie University, School of Medicine, Department of Psychiatry, Professor, 医学部, 教授 (10024720)

Co-Investigator(Kenkyū-buntansha) MURASE Sumio  Mie University, School of Medicine, Department of Physiology, Assistant, 医学部, 助手 (70200285)
OTANI Masato  Mie University, Faculty of Education, Assistant Professor, 教育学部, 助教授 (80185298)
KITAYAMA Isao  Mie University, University Hospital, Department of Psychiatry, Lecturer, 医学部附属病院, 講師 (70024784)
Project Period (FY) 1989 – 1991
KeywordsStress / Animal model of depression / Noradrenergic neuron / Locus coeruleus / Electron microscopy / Electrophysiology / In situ viewing / Plasticity
Research Abstract

Exposure of female rats to chronic forced walking stress led to an animal model which is analogous to human depression in terms of spontaneous activity, estrus cycle, glucocorticoids secretion and the effect of antidepressants. The function of the ascending noradrenaine (NA) neurons seemed to decrease in the model of depression in view of the decreased tyrosine hydroxylase (TH) activity, the decreased spontaneous firing rate, ultrastructural degenerative changes (destruction of the endoplasmic reticulum membrane etc.) of locus coeruleus (LC), beta-adrenergic receptor down-regulation and the increased NA content in the neurons. Chronic treatment with imipramine restored the spontaneous running activity, but did not the firing rate and the ultrastructural changes. The percentage of LC neurons antidromically activated by cortical stimulation decreased in the model animals. This observation also suggests an axonal degeneration of LC neurons projecting to the cerebral cortex. The fluorescence intensity of catecholamine in the neuron terminals observed by glyoxylic acid method increased in the paraventricular nucleus, the supraoptic nucleus and the hippocampus of the model rats. The structural change of NA neuron fibers in the cerebral cortex stained with dopamine beta-hydroxylase (DBH) antiserum and the percentage of LC neurons retrogradely traced by cortical injection of horseradish peroxydase (HRP) were examined, but their image analysis has not completed yet. In situ viewing of NA neuron terminals and cell bodies by injection of carbocyanine dye (DiI) is now performed. It was been confirmed that DiI injected to the cerebral cortex flows into the LC cells and stays there at least for 3 months.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 川口 茂: "うつ病モデルラットの視床下部・下垂体・副腎皮質系の機能ー免疫組織化学的研究ー" 精神薬療基金研究年報. 22. 151-155 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川口 茂: "うつ病モデルラットの視床下部・下垂体・副腎皮質機能:免疫組織化学的研究" 脳と精神の医学. 2. 505-509 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shouji NAKAMURA: "Electrophysiological evidence for axonal degeneration of locus coeruleus neurons following long-term forced running stress." Brain Research Bulletin. 26. 759-763 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyonori NAKAMURA: "Effect of forced-running stress on β-adrenergic receptors in rat brain regions and liver." Japaese Journal of Psychiatry and Neurology. 46. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大谷 正人(分担),高橋 三郎(編): "(本名)厚生省感情障害の成因と治療に関する研究平成2年度研究成果報通書 (分担名)鬱病モデルラットの弓状核,青斑核における超微細構造の変化と抗鬱薬の影響" 創造印刷, 109-114 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isao KTAYAMA(分担),Richard Kvetnansky(編): "(本名) Stress:Neuroendocrine and Molecular Approaches.(分担名) Noradrenergic and neuroendocrine function in a chronic walkingstressーinduced model of depression in rats." Gordon and Breach, 73-80 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KITAYAMA, I. et al.: "Chronic immobilization stress : evidence for decreases of 5-hydroxy-tryptamine immunoreactivity and for increases of glucocorticoid receptor immunoreactivity in various brain regions of the male rat." Journal of Neural Transmission. 77. 93-130 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OTANI, M.: "Fine structural changes in the rat arcuate neurons by forced running stress." Japanese Journal of Psychiatry and Neurology. 44. 99-110 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SHIBAHARA, K.: "Effect of forced-running stress on thyrotropin-releasing hormone in rat brain." Mie Medical Journal. 40. 91-97 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KAWAGUCHI, S.: "Hypothalamo-pituitary-adrenocortical function in the stress-induced depression model rat : Immunocytochemical studies." Mie Medical Journal. 40. 163-170 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAKAMURA, S. et al.: "Electrophysiological evidence for axonal degeneration of locus coeruleus neurons following long-term forced running stress." Brain Research Bulletin. 26. 759-763 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAKAMURA, T.: "Effect of forced-running stress on beta-adrenergic receptors in rat brain regions and liver." Japanese Journal of Psychiatry and Neurology. 46. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KITAYAMA, I. et al.: "Noradrenergic and neuroendocrine function in a chronic walking stress-induced model of depression in rats. In : Stress : Neuroendocrine and Molecular Approaches" Gordon and Breach. ed. by Kvetnansky, R. et al.73-80 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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