1991 Fiscal Year Final Research Report Summary
Characterization of receptors for human colony-stimulating factor (GM-CSF and IL-3)
| Project/Area Number |
01570675
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Tokyo University |
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Principal Investigator |
MIYAGAWA Kiyoshi Tokyo Univ., The 3rd Dept. of Int. Med., assistant professor, 医学部(病), 助手 (40200133)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMOTO Koichi Tokyo Univ., The 3rd Dept. of Int. Med., medical doctor, 医学部(医), 医院
TOYOSHIMA Hideo Tokyo Univ., The 3rd Dept. of Int. Med., assistant professor, 医学部(医), 助手 (20197966)
CHIBA Shigeru Tokyo Univ., The 3rd Dept. of Int. Med., medical doctor, 医学部(病), 医員
HIRAI Hisamaru Tokyo Univ., The 3rd Dept. of Int. Med., associate professor, 医学部(医), 講師 (90181130)
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| Project Period (FY) |
1989 – 1991
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| Keywords | colony-stimulating factor / IL-3 / GM-CSF / soluble receptor |
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| Research Abstract |
GM-CSF and EL-3 can act on a broad range of hematopoietic cells and support their growth and differentiation. Although their biological role is striking, little is known about the molecular mechanism of their intracellular signaling pathway. In this study, we have demonstrated that high affinity-binding site of GM-CSF is partially blocked by the addition of IL-3.
We have also shown that low affinity receptor for GM-CSF is expressed not only in hematopoietic cells but also in a human choriocarcinoma line. Furthen-nore, addition of GM-CSF to this cell line could induce the production of gonadotropin. Therefore, 1E is likely that low affinity receptor for GM-CSF itself can transduce some signals.
Recently, soluble type of receptors has been identified for many lymphokines. These soluble receptors are presumed to have significant biological role, such as competitive inhibitor of membrane receptor. We. could reveal that there is a soluble type of GMCSF receptor. A soluble protein of 62 kDa in the supernatant of a human choriocarcinoma cell line was shown to bind to ^<125>I-labeled GM-CSF. This binding was specifically blocked by the excess addition of non-labeled GM-CSF. We could not recognize this type of receptor in the supernatant of hematopoietic cells.
The structure and the biological role of this soluble GM-CSF receptor is currently under investigation.
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Research Products
(18 results)
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[Publications] Sugimoto, K., Toyoshima, H., Sakai, R., Miyagawa, K., Hagiwara, K., Hirai, H., Ishikawa, F., Takaku, F.: "Mutations of the p53 gene in lymphoid leukemia." Blood. 77. 1153-1156 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tanaka, T., Suda, T., Suda, J., Inoue, T., Hirabayashi, Y., Hirai, H., Takaku, F., Miura, Y.: "Stimulatory effects of granulocyte colony -stimulating factor on CFU-S differentiation and pre-CFU-S proliferation in mice." Blood. 77. 2597-2602 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Chiba, S., Takaku, F., Tange, T., Shibuya., K., Misawa, C., Sasaki, K., Miyagawa, K., Yazaki, Y., Hirai, H.: "Establishment and erythroid differentiation of a cytokine-dependent human leukemic cell line F-36 : A parental line requiring granulocyte-macrophage colony-stimulating factor or interleukin-3, and a subline requiring erythropoietin." Blood. 78. 2261-2268 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sugimoto, K., Toyoshima, H., Sakai, R., Miyagawa, K., Hagiwara, K., Ishikawa, F., Takaku F., Yazaki, Y., Hirai, H.: "Frequent mutations in the p53 gene in human myeloid leukemia cell lines." Blood.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hanazono, Y., Miyazono, K., Piao, Y-F., Taketazu, F., Chiba, S., Miyagawa, K., Hirai, H., Sakamoto, S., Miura, Y., Takaku, F.: Int. Hematol.
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hanazono, Y., Miyazono, K., Tsuchimochi, H., Yamaoki, K., Miyagawa, K., Yazaki, Y., Takaku, F.: "Treatment of infective endocarditis with granulocyte colony-stimulating factor." Jap. J. Med.30. 593-596 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Taketazu, F., Chiba, S., Shibuya, K., Kuwaki, T., Tsumura, H., Miyazono, K., Miyagawa, K., Takaku, F.: "IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor." J. Cell. Physiol.146. 251-257 (1991)
Description
「研究成果報告書概要(欧文)」より
