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1991 Fiscal Year Final Research Report Summary

Detection of HTLV-I genome in lymphocytes by DNA amplification method and its clinical significance.

Research Project

Project/Area Number 01570689
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionNagasaki University

Principal Investigator

FURUKAWA Koichi M. D.  Department of Oncology Nagasaki University School of Medicine Assistant Professor, 医学部, 助手 (80211530)

Co-Investigator(Kenkyū-buntansha) IKEDA Shuichi M. D.  Department of Internal Medicine, Nagasaki University School of Medicine Atomic B, 医学部, 助手 (10128150)
SHIKU Hiroshi M. D.  Department of Oncology Nagasaki University School of Medicine Professor, 医学部, 教授 (80154194)
Project Period (FY) 1989 – 1990
KeywordsDNA amplification method (PCR) / HTLV-I / adult T cell leukemia (ATL)
Research Abstract

1. pol region of HTLV-I integrated in the host DNA in ATL patients and HILV-I carriers was amplified by PCR method. Using a image analyzing of the products with 32P-labeled oligomer after dot hybridization the dose of HTLV-I genome was determined.
2. When DNA from a cell line containing one copy of HTLV-I, the diluted DNA and the intensity of dot hybridization of PCR products corresponded very well.
3. Based on these results, PBLs fram 134 cases of HTLV-I healthy carriers were analyzed. In the results, the negative cases were 1 %, less than 0.1 % equivalent was 18 %, 0.1-1 % equivalent was 34 %, 1-10 % equivalent was 42 %, more than 10 % was 5 the cases with negative antibody titer against HTLV-I showed negative in PCR.
4. When these results were analyzed based on the age, cases with less than 1 % equivalent decreased and cases with more. than 10 % equivalent increased with aging.
5. Viral genome dose in individual carriers correlated with the titer of anti-tax antibody.
6. When 22 samples from HAM patients were analyzed, they showed characteristic features in which low-dose group is less, and high-dose group is more than those of healthy carriers, suggesting that they are high responders.
7. When lymphoma-type ATL were analyzed, the lymph node cells showed high genetic dose and showed clonal bands in conventional Southern blot analysis. On the other hand, the PBLs of them showed generally low dose, and showed few clonal bands in Southern blot analysis. Thus, the possibility that ATL may occur fram even the individuals with low genome dose in PBLs.
8. As the genome dose showed high, the frequency of abnormal lymphocytes in peripheral blood became higher.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Takayama,K.et al: "Similarity of expression of low molecular weight G proteins,smg p21 A and ras p21,in normal and malignant human tissues." Cancer Res.51. 2223-2228 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.et al: "Analysis of the fine specificitues of 11 monoclonal antibodies reactive with type 2 blood group determinants." Molecular Immunol.27. 723-732 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.et al: "Tumor necrosis foctor enhances GD3 ganglioside expression in cultured human melacocytes." Arch.Biochem.Biophys.281. 70-75 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi,H.et al: "Human monoclonal antibody with dual GM2/GD2 pecificity derived from an immunized melanoma melanoma patient." Proc.Natl.Acad.Sci.USA. 87. 3333-3337 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.et al.: "Gangliosides in Melanomas.In Human melanoma.From basic research to clinical application." Springer-Verlag,Heidelberg., 557 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.et al.: "Carbohydrate antigens on human cancer cells recognized by human monoclonal antibodies.In Cancer chemotherapy.Challenges for the future.Vol.5." Excepta Medica,Tokyo., 364 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takayama, K. et al: "Similarity of expression of low molecular weight G proteins, smg p21 and ras p21, in normal and malignant tissues." Cancer Res.51. 2223-2228 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furukawa, K. et al: "Analysis of the fine specificities of 11 monoclonal antibodies reactive with type 2 blood group determinates." Molecular Immunol.27. 723-732 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furukawa, K. et al: "Tumor necrosis factor enhances GD3 ganglioside expression in cultured melanocytes." Arch. Biochem. Biophys.281. 70-75 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi H. et al: "Human monoclonal antibody with dual GM2/GD2 specificity derived from an immunized melanoma patient." Proc. Natl. Acad. Sci. USA. 87. 3333-3337 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furukawa, K. et al: "Gangliosides in melanomas." Human melanoma. From basic research to clinical application. Springer-Verlag, Heiderberg. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furukawa, K. et al: "Carbohydrate antigens on human cancer cells recognized by human monoclonal antibodies." Cancer Chemotherapy Challenges for the future.5. (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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