1991 Fiscal Year Final Research Report Summary
Cryopresrvation of Pancreatic Islet
| Project/Area Number |
01570750
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Fukui Medical School |
|---|
Principal Investigator |
NAKAGAWARA Gizo Faculty of Medicine, Fukui Medical School, Proffssor, 医学部, 教授 (10019549)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NOTE Masayuki Faculty of Medicine, Fukui Medical School, Research Associate, 医学部, 助手 (60189412)
|
|---|
| Project Period (FY) |
1989 – 1991
|
| Keywords | Pancreatic islets transplantation / Cryopresevation / Islet cell cluster / Immunoalteration / Dissociated pancreatic islet cells / Pseudoislet |
|---|
| Research Abstract |
To clarify the possibility of developing a rapid cooling rate for islet cryopreservation, we used a cooling rate of 25ﾟC/min for hamster pancreatic islet cryopreservation. The grafting of cryopreserved is islets normalized streptozotocin induced hyperglycemia following isoleneic transplantation. On the other hand, no prolongation of graft survivals in the case of the xenoteneic transplantation of hamster islets to rats was observed. The present findings indicate that hamster pancreatic islets can be successfully cryopreserved using a rapid cooling rate, however, it does not appear that this treatment reduces islet vulnerability to xenogeneic graft rejection.
For the purpose of islet storage and, if as its result it may be possible, the reduction of passenger leukocytes, isolated pancreatic islets were dissociated and then cryopreserved by using a programmable temperature controller. The present paper describes the morphological and biological studies of the islet cells cultured in vitro
… More
after freezing-thawing process. Isolated pancreatic islets from Fisher rats can be dissociated completely by using EDTA and dispose. These islet cells were also cryopreserved without major loss of their numbers and after thawing they were aggregated into pseudoislets in vitro culture(FCIC).
Histological studies of immunostaining with anti-insulin and antiglucagon antibodies demonstrated the distribution of cells containing these insulin and glucagon hormones, which was the same of that of nonfrozen control cultured islets. The test of glucose-stimulating insulin release from the pseudoislets resulted in the good response as the controls (CI : non-frozen cultured islet), When the insulin content was expressed on a per DNA basis there were no differences between the FCIC and CI, suggesting that the insulin content per islet cell was not altered after these process.
Now we concluded that dissected pancreatic islet cells can be cryopreserved without a loss of their biological abilities.
Moreover, there was a significant prolongation of survival time for FCIC in allogenic transplantation, and was different pattern of cell menbrane's protein, included antibody's protein. These results suggested the elimination or alteration of immunogenicity Less
|
Research Products
(8 results)
