1990 Fiscal Year Final Research Report Summary
Development of Hybrid Artificial Graft With Hierarchical Tissue Structure
| Project/Area Number |
01570797
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
MATSUDA Takehisa Head, Dept. of Bioengineering National Cardiovascular Center, 生体工学部, 部長 (60142189)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAICHI Shigeko Research Associate Dept. of Cardiovascular Diseases National Cardiovascular Cent, 病因部, 研究員 (00093930)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Hybrid Artificial Graft / Endothelial Cells / Smooth Muscle Cells |
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| Research Abstract |
Development of Hybrid Artificial Graft ; Fast Nass Culturing & Seeding on Compliant Small-Diameter Artificial Grft. The fast mass culturing of bovine endothelial cells (ECs) were successfully achieved by using collagen microsphere in a spiner flask under mild agitation. The seeded cells adhered and grew well on microspheres. The harvesting by tripsinization resulted in a quite high cell proliferation rate perunit volume. The collected cells were seeded on a compliant small-diameter polyurethane graft overlayered with an artificial basement membrame, which is compose a of type I collagen and dermatame sulfate complex gel. The resultant lumen surface resembles well the morphoqlogy of natural vessel.
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