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1991 Fiscal Year Final Research Report Summary

Studies of the phagocytic of Reticulo-Endothelial System in rat liver following thermal injury.

Research Project

Project/Area Number 01570864
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionHiroshima University

Principal Investigator

OHTANI Minako  Hiroshima Univ. Univ., Hospital Associate Professor, 医学部附属病院, 助教授 (10033995)

Co-Investigator(Kenkyū-buntansha) KAJIHARA Hiroki  Hiroshima Univ. Organization Office for College of Medical Tech. Professor, 医療技術短期大学部創設準備室, 教授 (20034184)
OKABAYASHI Kiyoshi  Hiroshima Univ. Univ., Hospital Assistant Professor, 医学部附属病院, 講師 (50127627)
Project Period (FY) 1989 – 1991
KeywordsShock phase in burns / Kupffer cells / Phagocytic activity / Endotoxin / White pulp of Spleen / Cell necrosis
Research Abstract

The reticuloendothelial system(RES)is an essential component of the non-specific host defense system which clears circulating debris, such as aggregated protein, damaged cells and fibrin from the bloodstream, in addition to removing blood-borne bacteria.
We studied changes in plasma haemoglobin levels and morphology of Kupffer cells in rats following lethal thermal injury. The plasma haemoglobin levels rapidly increased to a maximum level 15 minutes after injury and then rapidly decreased with time. However, the values were still higher 5 hours after injury than those found before injury. Soon after burning the Kupffer cells phagocytized not only circulating cell debris including erythrocyte membranes and degenerate leucocytes but also large amounts of haemoglobin. This phagocytized haemoglobin is considered to inhibit the generation of bactericidal-free radicals and to depress Kupffer cell function. The number of Kupffer cells was markedlly decreased 5 hours after thermal injury, and probably relates to the persistent depletion of the reticuloendothilial system function which follows lethal thermal injury. In contrast to the decreased number of Kupffer cells, a small number of monocytic cells appeared in the sinusoidal spaces and adhered to the endothelial cells.
Our findings suggest that blood monocutes might migrate to the sinusoidal wall and transform into Kupffer cells. We also studied the effect of endotoxin on Kupffer cells in the rat liver following 30% thermal injury of body surface area. The Kupffer cells were markedly degenerate in the early stage.
Structural changes of the white pulp of the spleen were investigated in rats following thermal injury. Tingible bodies and tingible body macrophages were abundant in germinal center, follicular are and perifollicullar zone.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 岡林 清司他: "Structural changes of kupffer cells in the rat liver following experimental thermal injury." Burns. 16. 83-88 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Okabayashi et al: "Structural changes of Kupffer cells in rat liver following experimental thermal injury." Burns. 16-2. 83-88 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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