1990 Fiscal Year Final Research Report Summary
Basic study on the differentiation induction of human germ cell tumors and its clinical application
| Project/Area Number |
01570919
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
SEKIYA Souei Chiba University, Department of Obstetrics and Gynecology, Assistant professor, 医学部, 助教授 (00092065)
|
|---|
| Project Period (FY) |
1989 – 1990
|
| Keywords | germ cell tumor / HMBA / N-myc / neuron specific enolase / neural rosette / extracellular matrix |
|---|
| Research Abstract |
1. A plupribotent human embryonal carcinoma cell line (NEC14) could be induced to differentiate morphologically by treatment with 10^<-2>M N, N'-hexamethylene-bis-acetamide (HMBA) in vitro. The differentiation accompanied the marked induction of human major histocompatibility antigens (HLA-A, B, C), and the changes of stage specific embryonic antigens (from SSEA-1^-/SSEA-3^+ to SSEA-1^+/SSEA-3^-). Differentiated NEC14 cells expressed mesenchym-associated molecules, vimentin and tenascin ; thus HMBA can induce these cells to certain mesenchymalle elements of embryonal mesoderm.
2. The level of N-myc expression was high in undifferentiated NEC14 cells, but decreased transiently to less than 1/10 of the original level after the HMBA induction. To investigate the role of N-myc gene in the NEC14 cell differentiation, we established several NEC14 transformants expressing exogenous N-myc genes. They showed shorter population doubling time, increased plating efficiency and tumorigenic potential.
3. The level of neuron specific enolase (NSE) in serum was found to be a novel diagnostic marker for immature teratomas and dysgerminomas.
4. From the human teratocarcinoma line PA-1, we established a clonal line that produced a distinct neural rosettes within in vitro multicellular spheroids. This line expressed neuroectodermal antigens such as A2B5, HNK-1 and NC-1, NSE, S-100 protein and vimentin. These rosette showed a strikingly polarized and overlapped deposition of extracellular matrix components including laminin, type IV collagen, heparan sulfate proteoglycan and tenascin.
|
-
-
-
-
-
-
[Publications] Sekiya, S., Kimura, H., Yamazawa, K., Kera, K., Kawata, M., Takamizawa. H., and Oda, K.: "Induction of human embryonal carcinoma cell differentiation using N, N'-Hexamethylene Bisacetamide in vitro. Gynecol." Oncol.36. 69-78 (1990)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Hasegawa, T., Nakada, S., Nakajima, T., Oda, K., Kawata, M., Kimura, H. and Sekiya, S.: "Expression of various viral and cellular enhancer-promoters during differentiation of human embryonal carcinoma cells." Differentiation. 42. 191-198 (1990)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Hasegawa, T., Hara, E., Takehana, K., Nakada, S., Oda, K., Kawata, M., Kimura, H., and Sekiya, S.: "A transient decrease in N-myc expression and its biological role during differentiation of human embryonal carcinoma cells." Differentiation.
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Kawata, M., Sekiya, S., Kera, K., Kimura, H., and Takamizawa, H.: "Neural rosette formation within in vitro spheroids of a clonal human teratocarcinoma cell line, PA-1 NR : role of extracellular matrix components in the morphogenesis." Cancer Res.
Description
「研究成果報告書概要(欧文)」より
