1990 Fiscal Year Final Research Report Summary
Physiological Role of Alfa-Anp in Fetal Growth.
| Project/Area Number |
01570925
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Mie University |
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Principal Investigator |
SUGIYAMA Youichi Mie University Sch. of Med. Professor, 医学部, 教授 (30093131)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAYA Ken Mie University Sch. of Med. Instructor, 医学部, 助手 (60206399)
NISHIYAMA Masato Mie University Sch. of Med. Instructor, 医学部附属病院, 助手 (10218224)
YAMAMOTO Toshihiko Mie University Sch. of Med. Assistant Professor, 医学部, 講師 (80135459)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Fetal membrane / Decidual cell / Alfa-ANP / cGMP / Thymidine / Prolactin / Electrolyte / Osmotic pressure |
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| Research Abstract |
The results were as follows ; (1) The localization of Alfa-ANP was demonstrated in extra-villous trophoblasts, amniotic cells, and decidual cells by avidin-biotin peroxidase complex method. (2) Scatchard plots revealed a decidual cell possesses single kind of alfa-ANP receptor with Kd of 135 pM and Bmax of 81 pM/10^6 cells. (3)The concentration of cGMP WAS dose-dependently increased by the addition of alfa-ANP (-15 times). (4)alfa-ANP enhanced thymidine uptake by decidual cells. (5)when a double-chamber method using fetal membrane was employed, alfa-ANP induced significant differences of electrolytes and osmotic pressure between fetal hemi-cham-ber and maternal hemi-chamber. (6)The addition of alfa-ANP into the maternal hemi-chamber significantly increased the concentration of prolactin in the fetal hemi-chamber.
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