1990 Fiscal Year Final Research Report Summary
Analysis of the Reproductive Failure by Using Monoclonal Antibodies Against Human Sperm
| Project/Area Number |
01570929
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University Medical School |
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Principal Investigator |
SAJI Fumitaka Osaka University Medical School Department of Obstetrics and Gynecology Assistant Professor, 医学部, 講師 (90093418)
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| Co-Investigator(Kenkyū-buntansha) |
SAMEJIMA Yoshihiro Osaka University Medical School Department of Obstetrics and Gynecology Clinical, 医学部, 助手
OHASHI Kazutomo Osaka University Medical School Department of Obstetrics and Gynecology Clinical, 医学部, 助手 (30203897)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Antisperm antibody / Infertility / Capacitation / ELISA / Sperm immobilizing test / monoclonal antibody / acrosomal reaction |
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| Research Abstract |
1. Screening tests for antisperm antibodies were performed on 684 infertile women by Sperm Agglutination test (SAT), sperm immobilization test (SIT), enzyme-linked immunosorbent assay (ELISA), immunobeads test and mixed antiglobulin reaction (MAR). Seventy-one patients with circulating antisperm antibodies were selected by these methods.
2. The monoclonal antibodies against human sperm were obtained by using Balb/c mouse spleen cells immunized with capacitated human sperm. All antibodies showed inhibitory effects on Zona free hamster egg-human sperm penetration test (ZSPT).
3. Monoclonal antibody, MH61, which reacts specifically with capacitated human sperm was conjugated with Dynabeads M-450. A remarkable agglutination of MH61 coated beads was observed after incubation with sperm of fertile men, while poor agglutination activity was observed in sperm of infertile patients.
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