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1991 Fiscal Year Final Research Report Summary

Isolation and characterization of an novel oncodevelopmentalprotein from human malignant ascitic fluid.

Research Project

Project/Area Number 01570939
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKitasato University School of Medicine

Principal Investigator

NAKAI Mitsuo  Kitasato University School of Medicin, Dept. of OB/GYN., Professor, 医学部, 教授 (50050399)

Co-Investigator(Kenkyū-buntansha) IINO Joji  北里大学, 医学部, 助手 (30176052)
ISHIKAWA Masakazu  北里大学, 医学部, 助手 (40184494)
YAMAURA Noboru  北里大学, 看護学部, 助教授 (60050621)
Project Period (FY) 1989 – 1991
KeywordsTumor-associated protein / Oncedevelopmental protein / Pregnancy-associated protein / Tumor marker / Isolation / Characterization / Serum concentration / Gynecological malignant diseases
Research Abstract

A tumor-associated antigen that shares antigenicity with a pregnancy-associated protein has been detected in ascitic fluid of patients with advanced ovarian can cer. The protein was prepared from malignant ascitic fluid by the combination of poly (ethylene glycol) 4000 fractionation, DEAE-Sepharose column chromatography and prepardtive polyacrylamide gel electrophresis (PAGE). Antiserum against an malignant ascitic antigen (MASA) was raised in rabbits. The rabbit antiserum was applied to a column of normal human plasma (NHP)-coupled Sepharose, and specific antibodies against MASA (anti-MAsA) was highly purified. Subsequently, by the use of anti-MAsA-coupled Sepharose, highly purified MASA was obtained. The preparation thus obtained was analyzed on sodium dodecyl sulfate (SDS)-PAGE, Using this technique, a single antigenically active component was identified" The moleculermass of this MASA species was calculated to be 27 kDa by SDS-PAGE.
Highly sensitive assay method employed ELISA was established by the use of the monospecific anti-MAsA. With this methody- the smallest detectable amount detectable in a sample was 0.00125 U/ml. The sensitivity 6f the ELISA is 1, 000-fold higher than that of a previous electroimmunoassay employed a modified Laurell's method. With this method, it will be possible to establish the normal level range, which is a prerequisite for precise evaluation of the increase in abnormal conditions. In conclusion, the present study shows that estimation of the MASA will be an usefulladfunct for the management of patients with gynecological malignant diseases.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] NAKAI M,ATSUMI M,SONE Y and ARAI M: "Quantitative estimation of an alpha 2 pregnancy-asociated protein in normal preqnancy by means of an electroimmunoassay." Clinica Chimica Acta. 182. 103-109 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石川 雄一,剣持 稔,黒須 不二男,中居 光生: "悪性腹水における新しい腫瘍関連蛋白の精製と性質について" 第10回腫瘍マ-カ-研究会記録. (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 馬嶋 恒雄 中居 光生: "新しい妊娠関連蛋白の単純放射免疫拡散による測定法の考案と妊娠時動態" 日本不妊学会雑誌. 36. 101-106 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NAKAI M,ISHIKAWA M and KAWAUCHI H: "Identification of an ectopic developmental antigen that appears in malignant asctitic fluid." European Journal of Obstetrics & Gynecology and Reproductive Biology. 42. 61-69 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NAKAI M,KAWAUCHI H,ATSUMI M,MAJIMA,T and ISHIKAWA M: "A novel tumor-associated protein:clinical significance of serum levels in various clinical condutions with special reference to gynecological malignant diseases." European Journal of Obsterics & Gynecology and Reproductive Biology. 43. 143-147 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中居 光生: "新しい「がん発生蛋白」の特性解析とその臨床知見" 病態生理.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 馬嶋 恒雄: "新しい「がん発生蛋白」の血中濃度測定の臨床的意義ー産婦人科諸種病態とくに悪性における動態ー" 日本産科婦人科学会誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakai M: "Quantitative estimation of an alpha 2 pregnancy-associated protein in normal pregnancy by means of an electroimmunoassay." Clin Chim Acta. 182. 103-109 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa M: "Purification and characterization of an novel tumor-associated protein in malignanat ascitic fluid." Proceedings of the Xth Annual Meeting of the Tumor Marker.226-228 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Majima T: "Quantitation of a new pregnancy-associated protein in pregnancy sera by means of a single redial immunodiffusion technique." Jpn J Fertil Steril. 36. 444-451 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakai M: "Identification of an ectopic developmental antigen that appears in malignant ascitic fluid." Eur J Obstet Gynecol Reprod Biol. 42. 61-69 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kami K: "Immunocytochemical investigation of an ectopic developmental antigen (a_2-PAP) in human placentae." Acta Anat Nippon. 66. 290 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakai M: "A Novel tumor-associated protein : clinical significance of serum levels in various clinical conditions with special reference to gynecological malignant diseases." Eur J Obstet Gynecol ReproduBiol. 43. 143-147 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakai M: "Characterization and clinical application of an novel oncodevelopmental protein" Medicine phylosophica.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kami K: "Cell-biological study of a novel tumor-associated ascitic antigen." Acta Histochem Cytochem. 24. 518 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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