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1991 Fiscal Year Final Research Report Summary

Pathophysiology of Mucociliary System in Otitis Media with Effusion

Research Project

Project/Area Number 01570959
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionOsaka City University

Principal Investigator

OHASHI Yoshihiro  Osaka City University Medical School, Lecturer, 医学部, 講師 (60160602)

Co-Investigator(Kenkyū-buntansha) MASUTANI Haruhiko  Osaka City University Medical School, Assistant Professor, 医学部, 助手 (10190357)
KONISHI Kazuo  Osaka City University Medical School, Assistant Professor, 医学部, 助手 (50145801)
Project Period (FY) 1989 – 1991
Keywordsotitis media with effusion / mucociliary system / endotoxin / influenza A virus / middle ear effusion / ciliary activity / medication
Research Abstract

Guinea pigs were treated with intratympanic instillation of 1) S. aureus, 2) bacterial endotoxin, 3) influenza A, histamine, and 5) platelet-activating factor (PAF). Guinea pigs were also treated with x-ray irradiation and exposure to nitrogen dioxide. Ilese factors except for intratympanic inoculation of S. aureus could result in experimental outis media with effusion (OME). Intratympanic instillation of endotoxin, influenza A, histamine, and PAF induced self-limiting OME in guinea pigs. On the other hand, irradation and nitrogen dioxide exposure induced prolonged OME in guinea pigs. The following results were obtained from functional as well as morphological examination of the tubotympanic mucociliary systrm in the above-mentioned animals. Thee pathological factors are involved in the pathogenesis of OME, which included (1) increased number and/or activity of goblet cells, (2) increased exudation due to an increased vascular permeability, and (3) reduction in ciliary activity. Middle … More ear effusions were retained reduction in ciliary activity was superposed on either of (1) increased number and/or actibvity of goblet cells or (2) increased exudation due to an increased vascular permeability. Our stud has also demonstrated that cilia locating in the eustachian tube and the middle ear close to the tympanic orifice play a significant role in the clearance of surplus fluid in the tubotympanum. On the other hand, cilia locating in the supen'or bulla had no important role in the tubotympanic mucociliaiy clearance. Middle ear effusions were cleared to the pharynx when cilia in the eustachian tube and the middle ear near the orifice recovered. their beadng activity. An inference has been derived from the experimental fact that medication leading to normalization or enhancement of ciliaiy activity in such cilia should alleviate, OME.
We also gave some mediation whose ciliostimulatory effects are, confinned by our pervious study, to guinea pigs with OME. Up to date, we confirmed that 2 kinds of medicine (S-carboxymethylcysteine and Sai-Rei-To) could stimulate ciliary activity, resulting in resolution of middle ear effusions. Our clinical tdal using these two medicines are going to confirm clinical usefulness of these medicines. Less

  • Research Products

    (63 results)

All Other

All Publications (63 results)

  • [Publications] 大橋 淑宏: "I型アレルギ-反応の中耳・耳管線毛運動に及ぼす影響に関する実験的研究" Ear Res.Jpn.20. 19-24 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大橋 淑宏: "日常診療に役立つ高齢化にともなう耳鼻咽喉科疾患の診断と治療ー老人の滲出性中耳炎ー" 大阪府耳鼻咽喉科医会会報. 32. 15-17 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大橋 淑宏: "I型アレルギ-反応の中耳・耳管粘膜線毛運動に及ぼす影響" Ear Res.Jpn.20. 177-178 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "An experimental study of mucociliary pathology of the eustachian tube in otitis media with effusion induced by irradiation." Arch.Otorhinolaryngol.246. 428-432 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Acute effects of sulfur dioxide exposure on the middle ear mucosa." Ann.Otol.Rhinol.Layngol.98. 301-307 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Mucociliary disease of the middle ear during exprimental otitis media with effusion induced by bacterial endotoxin." Ann.Otol.Rhinol.Laryngol.98. 479-484 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Increased ciliary beating frequency of nasal mucosa following immunotherapy for allergy." Ann.Otol.Rhinol.Layngol.98. 350-354 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "InfluenzaA virus-induced mucociliary pathology of the tubotympanum." J.Clin.Electron Microscopy. 22. 902-903 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.J.Lim: "Structural organization of the outer hair cell wall." Acta Otolaryngol(Stockh).107. 398-405 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] L.O.Bakaletz: "Effect of formalin-fixed Hemophilus influenzae and Streptococcus pneumoniae on dye transport by the chinchilla eustachian tube." Acta Otolaryngol(Stockh).107. 235-245 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.J.Lim: "The animal model for influenza A otitis media." Ped.Inf.Dis.Journ.8. 38-40 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 江崎 裕介: "滲出性中耳炎症例における上咽頭粘膜の線毛運動機能" Ear Res.Jpn.22. 177-178 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 江崎 裕介: "エンドトキシンの中耳・耳管線毛機能障害に関する研究" Ear Res.Jpn.20. 373-374 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 江崎 裕介: "生理活性物質の線毛運動に及ぼす影響" 頭頚部自律神経. 3. 13 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 江崎 裕介: "柴苓場による中耳線毛運動賦活作用" 耳鼻臨床. 82. 1791-1795 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Esaki: "Experimental otitis media with effusion induced by lipopolysaccharide from Klebsiella pneumoniae." J.Clin.Electron Microscopy. 22. 599-600 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉浦 欣一: "ヒスタミンの中耳・耳管粘液線毛機能に及ぼす影響" Ear Res.Jpn.20. 363-364 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Influence of human middle ear effusion on guinea pig middle ear mucosa." J.Clin.Electron Microscopy. 23. 954-955 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大橋 淑宏: "インフルエンザウイルスの鼓室内注入後のチンチラ耳管粘膜病態の定量的観察" 日本耳鼻咽喉科感染症研究会会誌. 8. 12-15 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大橋 淑宏: "柴苓湯の実験的滲出性中耳炎に及ぼす影響" 臨床耳科. 17. 189 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohno: "The role of virus in endotoxin-induced otitis media with effusion." J.Clin.Electron Microscopy. 23. 948-949 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大野 義春: "ウイルスの培養耳管線毛運動に及ぼす影響" 耳鼻臨床. 83. 1243-1247 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大野 義春: "中耳貯留液の線毛機能に及ぼす影響に関する実験的研究" Ear Res.Jpn.21. 39-40 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大野 義春: "ヒト中耳貯留液の中耳・耳管粘膜に及ぼす影響" 臨床耳科. 17. 78 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉浦 欣一: "滲出性中耳炎におけるウイルスの関与に関する実験的研究" Ear Res.Jpn.21. 61-62 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Okamoto: "Effects of Sai-Rei-To on mucociliary pathology in experimental otitis media with effusion." J.Clin.Electron Microscopy. 23. 946-947 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大橋 淑宏: "ヒスタミンの鼓室内注入による実験的滲出性中耳炎の粘膜病態に関する研究" Otol.Jpn.1. 7-13 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大橋 淑宏: "PAFの中耳・耳管粘膜に及ぼす影響に関する実験的研究" Otol.Jpn.1. 166 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Experimental otitis media with effusion induced by lipopolysaccharide from Klebsiella pneumoniae.-Mucociliary pathology of eustachian tube." Acta Otolaryngol(Stockh). supp/486. 105-115 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Influenza A virus-induced otitis media and mucociliary dysfunction in the guinea pig." Acta Otolaryngol(Stockh). supp/486. 135-148 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Current concept of mucociliary dysfunction in otitis media witheffusion." Acta Otolaryngol(Stockh). supp/486. 149-161 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Ciliary activity in the tubotympanum during type I allergic reaction." Acta Otolaryngol(Stockh). supp/486. 162-175 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Esaki: "Histamine-induced mucociliary dysfunction and otitis media with effusion." Acta Otolaryngol(Stockh). supp/486. 116-134 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Onho: "Platelet-activating factor-induced mucociliary dysfuntion in guinea pig." Allergy Clin.Immunol.News. supp/1. 377 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉浦 欣一: "インフルエンザウイルスの中耳・耳管粘液線毛系に及ぼす影響に関する実験的研究" 日耳鼻. 94. 506-515 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡本 英樹: "二酸化窒素暴露の中耳・耳管粘膜に及ぼす影響に関する実験的研究" Otol.Jpn.1. 107 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大橋 淑宏: "ヒト中耳貯留液のモルモット中耳・耳管粘液線毛系に及ぼす影響に関する実験的研究" 臨床電顕誌. 24. 247-262 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Ultrastructural pathology of the tubotympanum following experimental influenza A virus-induced otitis media." Am.J.Otolaryngol.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大橋 淑宏: "実験的滲出性中耳炎の粘膜病態と薬物療法ー柴苓場の効果に関する研究ー" 耳鼻臨床.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohno: "Influence of plantelet-activating factor on the tubotympanum." J.Clin.Electron Microscopy.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ohashi: "Recent Advances in Otitis Media" D.J.Lim.C.D.Bluestone,J.O.Klein,D.J.Nelson,

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Okamoto: "Recent Advances in Otitis Media" D.J.Lim,C.D.Bluestone,J.O.Klein,D.J.Nelson,

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Ohashi: "An experimental study of mucociliary pathology of the eustachian tube in otitis media with effusion induced by irradiation." Arch. Otorhinolaryngol. 246. 428-432 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Acute effect of sulfur dioxide exposure on the middle ear mucosa." Ann. Otol. Rhinol. Laryngol. 98. 301-307 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Mucociliary disease of the middle ear during experimental otitis media with effusion induced by bacterial endotoxin." Ann. Otol. Rhinol. Laryngol. 98. 479-484 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Increased ciliary beating frequency of nasal mucosa following immunotherapy for allergy." Ann. Otol. Rhinol. Laryngol. 98. 350-354 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Influenza A virus-induced pathology of the tubotympanum." J. Clin. Electron Microscopy. 22. 902-903 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D. J. Lim: "Structural organization of the outer hair cell wall." Acta Otolaryngol (Stockh).107. 398-405 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] L. O. Bakaletz: "Effect of formalin-fixed Hemophilus influenza and Streptococcus pneumonia on dye transport by the chinchilla eustachian tube." Acta Otolaryngol (Stockh).107. 235-245

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D. J. Lim: "The animal model for influenza A otitis media." Ped. Inf. Dis. Journ.8. 38-40 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Esaki: "Experimental otitis media with effusion induced by lipopolysaccharide from Klebsiella pneumonia." J. Clin. Electron Microscopy. 22. 599-600 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Influence of human middle ear effusion on guinea pig middle ear mucosa." J. Clin. Electron Microscopy. 23. 954-955 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohno: "The role of virus in endotoxin-induced otitis media with effusion." J. Clin. Electron Microscopy. 23. 948-949 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Okamoto: "Effect of Sai-Rei-To on mucociliary pathology in experimental otitis media with effusion." J. Clin. Electron Microscopy. 23. 946-947 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Experimental otitis media with effusion induced by lipopolysaccharide from Klebsiella pneumonia. -Mucociliary pathology of the eustachian tube." Acta Otolaryngol (Stockh).Suppl 486. 105-115 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Influenza A virus-induced otitis media and mucociliary dysfunction in the guinea pig." Acta Otolaryngol (Stockh).Suppl 486. 135-148 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Ciliary activity in the tubotympanum during type I allergic reaction." Acta Otolaryngol (Stockh).Suppl 486. 162-175 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Esaki: "Histamine-induced mucociliary dysfunction and otitis media with effusion." Acta Otolaryngol (Stockh).Suppl 486. 116-134 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohno: "Platelet-activating factor-induced mucociliary dysfunction in the" Allergy Clin. Immunol. News. Suppl 1. 377 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Ultrastructural pathology of the tubotympanum following experimental influenza A virus-induced otitis media." Am. J. Otolaryngol.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohno: "Influence of platelet-activating factor on the tubotympanum." J. Clin. Electron Microscopy.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Ohashi: "Recent advances in otitis media"

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Okamoto: "Recent advances in otitis media"

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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