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1990 Fiscal Year Final Research Report Summary

The Study of Establishment on Immunobiological Evaluation of Endodontic Materials and Medicaments

Research Project

Project/Area Number 01571056
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

OSADA Tamotsu  Kanagawa Dental College Endodontics Professor, 歯学部, 教授 (70084720)

Co-Investigator(Kenkyū-buntansha) TANI Nobutuki  Kanagawa Dental College, Endodontics, Instructor, 歯学部, 助手 (20163610)
TANI Nobutuki  Kanagawa Dental College, Endodontics, Instructor (20163610)
TANI Nobutuki  Kanagawa Dental College, Endodontics, Instructor (20163610)
TANI Nobutuki  Kanagawa Dental College, Endodontics, Instructor (20163610)
TANI Nobutuki  Kanagawa Dental College, Endodontics, Instructor (20163610)
Project Period (FY) 1989 – 1990
KeywordsPulp Capping Materials / IL-1 / Root Canal Filling Materials / PGE_2 / Collagenase / HPF / HPLF / 歯根膜細胞
Research Abstract

The present study was conducted to assess the biological effects of seven pulp capping materials (Dycal, Life, NU-CAP, Cavitek, EUGEDAIN, Fuji-Ionomer Type II and Base cement) against human pulp fibroblasts (HPF), and six root canal filling materials (CANALS, NU-59, AH-26, Endo-fill, Sealapex, APATITE ROOTSEALER TYPE-II). The cell-biological effects were performed by measuring IL-1, PGE_2, Collagenase, DNA synthesis, Cell viabilities and Alkaline phosphatase (ALP) activity of HPF or HPLF stimulated with dissolution of each material.
The results by Pulp Capping Materials obtained were as follows.
1) The production of IL-1alpha and IL-1beta stimulated with Fuji-Ionomer Type II were significantly high.
2) The PGE_2 production was induced when HPF was stimulated by all test samples, especially increased by stimulation with Dycal.
3) The Collagenase activity of HPF was not induced by stimulation with all test samples.
4) The ALP activities from HPF stimulated with Dycal, Life, and Cavitek were f … More ound.
5) All materials strongly inhibited DNA synthesis of HPF at 10^0 dilution, however all materials except Base cement and Fuji-Ionomer Type-II at 10^<-2> dilution were found to have little inhibition. The inhibition of DNA synthesis by all materials depended on the concentration between 10^<-2> and 10^0 dilution.
6) Although Fuji-Ionomer Type II inhibited the growth of HPF distinctly, the viabilities of HPF stimulated with each material depended on the concentration between 10^<-2> and 10^0 dilution.
The results by Root Canal Filling Materials obtained were as follows.
1) The production of IL-1 beta from HPLF stimulated APATITE ROOTSEALER TYPE II and Endo-fill were determinable, but the production of IL-1 alpha was not determinable at all.
2) The PGE_2 production was induced when HPLF was stimulated by all test samples, especially increased by stimulation with CANALS.
3) The Collagenase activity of HPLF was not induced by stimulation with all test samples.
4) The ALP activities from HPLF stimulated with Sealapex, CANALS and APATITE ROOTSEALER TYPE-II were found.
5) All test samples strongly inhibited DNA synthesis of HPLF at 10^0 dilution, however all materials at 10_<-2> dilution were found to have little inhibition.
6) All test samples inhibited the growth of HPLF, the viabilities of HPLF stimulated with each materials depended on the concentration between 10^<-2> and 10^0 dilution. Less

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 三上 昌子、谷 信之、冨永 尚宏、長田 保、梅本 俊夫: "覆髄材の起炎性に関する細胞生物学的研究" 日本歯科保存学雑誌. 33. 584-592 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三上 昌子、谷 信之、長田 保、梅本 俊夫: "ヒト歯髄細胞の増殖とアルカリフォスファタ-ゼ活性に及ぼす覆髄材の影響(掲載予定)" 日本歯科俊存学雑誌. 34. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MASAKO MIKAMI, NOBUYUKI TANI, NAOHIRO TOMINAGA, TAMOTSU OSADA, TOSHIO UMEMOTO: "Cell Biological Studies on the Effects of Pulp Capping Materials on Production of Chemical Mediators from Human Pulp Fibroblast" Japanese Journal of Conservative Dentistry. Vol. 33. NO. 2. 584-592 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MASAKO MIKAMI, NOBUYUKI TANI, TAMOTSU OSADA, TOSHIO UMEMOTO: "Effects of Pulp-Capping Materials on Growth and Alkaline Phosphatase Activity of Human Pulp Fibroblasts" Japanese Journal of Conservative Dentistry. Vol. 34. NO. 2. (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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