A STUDY OF INFLAMED TISSUE REMODELING IN THE HEALING PROCESS ; THE REGULATION OF COLLAGENASE ACTIVITY

1990 Fiscal Year Final Research Report Summary

• Project/Area Number: 01571082
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 外科・放射線系歯学
• Research Institution: HOKKAIDO UNIVERSITY
• Principal Investigator: MIKOYA Tadashi SCHOOL OF DENTISTRY, HOKKAIDO UNIVERSITY INSTRUCTOR, 歯学部, 助手 (10181869)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Ichizo SCHOOL OF DENTISTRY, HOKKAIDO UNIVERSITY INSTRUCTOR, 歯学部, 助手 (70170305) ; NISHIKATA Satoshi SCHOOL OF DENTISTRY, HOKKAIDO UNIVERSITY INSTRUCTOR, 歯学部, 助手 (00125337)
• Project Period (FY): 1989 – 1990
• Keywords: Inflamed tissue remodeling / Collagen metabolisum / Collagenase activation / Fibrinolytic system / Crosslinks of collagen / Carrageenin / Granulation tissue / Tranexamic acid

Research Abstract

To elucidate collagen metabolisum during the healing process of inflammation, the relationship between a fibrinolytic system and collagenase activation in vivo, and the susceptibility to collagenase by crosslinkings were studied using carrageenin-in13EA\ : duced granulation tissue in rats.
When fibrinolysis was inhibited by the administration of tranexamic acid to rats, the ratio of latent form collagenase to active form collagenase in granulation tissue increased, though the activity of active form collagenase decreased. This result indicates that the activation of latent form collagenase in the healing tissue of rat depends on a fibrinolytic system.
Then, in all stages, the major reducible crosslinks of collagen in granulation tissue were dihydroxylysinonorleucine (DHLNL) and hydroxylysinonorleucine (HLNL). The ratio of DHLNL/HLNL on the 7th day was the highest value compared with its on any ot13EA\ : her stages. This result suggests that most active biosynthesis and fibrogenesis of granulation tissue collagen occurs in this stage.