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1991 Fiscal Year Final Research Report Summary

Evaluation of cell kinetics in oral and maxillofacial tumors

Research Project

Project/Area Number 01571094
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionYamaguchi University

Principal Investigator

SHINOZAKI Fumihiko  Yamaguchi University School of Medicine, Professor, 医学部, 教授 (90045443)

Project Period (FY) 1989 – 1991
KeywordsKi-67 / immunohistochemistry / proliferative rate / flow cytometer / oral and maxillifacial tumor / AgNOR / BrdUrd / proliferative marker
Research Abstract

Proliferative activity in oral and maxillofacial tumors have been examined using 4 different biological parameters in order to use these parameters for effective cancer treatments. The methods and parameters used are as follows : 1. S-phase fractions by in vitro bromodeoxy uridine labeling index (BrdU LI). 2. growth fraction by immunohistochemical technique using Ki-67 antibody (Ki-67LI). 3. DNA ploidy by flow cytometer. 4. Cellular activities by the number of AgNOR. An analysis of proliferative activity was shown much quicker and easier using BrdU LI, Ki-67 LI. Ki-67 Li reveloas the cycling cells without using any DNA precursors. We developed the criteria for counting AgNOR spots with experiments using cell line. The number of AgNORs per cells was possible to differentiate benign and malignant salivary gland tumors. The number of AgNOR showed significant correlation with the tumor respo nse to anti-cancer medicine, although other 3 parameters did not. BrdU LI were paralleled to Ki-67LI and lightly correlated with the number of AgNORs. DNA ploidy level had no relationship to these three parameters. The rate of cell proliferation ploys an important role in tumor behaviorl. It is likely that a few specific genetic changes can lead to such proliferative advantage. These may include changes such as mutation, deletion or amplification. Therefore we continue to investigate the relationship between genetypic and phenotioic diversity in oral and maxillofacial tumors. Fluorescence in situ hybridization (FISH) using repeated and unique sequence probes is used for analysis of chromosomal alteration such as aneusomy, amplification and deletion.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Matsumura,K: "The nucleolar organizer regions associated protein (AgNORs) in salivary gland tumors." Int.J.Oral Maxillofac.Surg.18. 76-78 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumura,K: "Immunohistochemical determination of growth fraction in human tumors." Path.Res.Prac.184. 609-613 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松村 耕治: "免疫組織化学的Bradllrd染色:DNaseによるDNA変性法の検討" 山口医学. 38. 163-169 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasaki,K: "Effects of serum deprivation on Ki-67 reactive antigen expression in HeLa cells." Acta Pathologica Japonica. 39. 638-642 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumura,K: "Evaluation of frozen section diagnosis and rapid intraoperative flow cytometric analysis of oral and maxillofacial lesions." Bull.Yamaguchi Med.Sch.37. 113-117 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumura,K: "Changes of AgNORs in HeLa cells during serum starvation." Br.J.Cancer. 62. 358-387 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 篠崎 文彦(分担): "口腔癌" 富田 隆策, 519 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumura, K: "The nucleolar organizer regions associated protein (AgNORs) in salivary gland tumors" Int. J. Oral Maxillo-fac. Sug. 18. 76-78 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumura, K: "Immunohistochemical determination of growth fraction in human tumors" Path. Res. Prac.184. 609-613 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki, K: "Effects of serum deprivation on ki-67 reactive antigen expression in HeLa cells" Acta. Pathologica Japonica. 39. 638-642 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumura, K: "Immunological staining of BrdUrd-Evaluation of DNA denaturation using DNase (in Japanese)" J. Yamaguchi Med.38. 163-169 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumura, K: "Changes of AgNORs in HeLa cells during serum starvation" Br. J. Cancer. 62. 385-387 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumura, K: "Evaluation of frozen section diagnosis and rapid intraoperative flow cytometric analysis of oral and maxillofacial lesions" Bull. Yamaguchi Med. Sch.37. 113-117 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinozaki, F: "Oral Cancer (in Japanese)" Dental Diamond Co. Tokyo Japan. 106-113 (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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