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1990 Fiscal Year Final Research Report Summary

Factors of Interaction between Osteoblasts and Bone Marrow Cells in Bone Remodeling

Research Project

Project/Area Number 01571113
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 小児・社会系歯学
Research InstitutionTohoku University

Principal Investigator

MITANI Hideo  Tohoku University School of Dentistry, Professor, 歯学部, 教授 (50014220)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Yoshiyuki  Tohoku University School of Dentistry, Assistant Professor, 歯学部, 講師 (20187470)
SHIMIZU Yoshinobu  Tohoku University, School of Dentistry, Associate Professor, 歯学部, 助教授 (20005078)
Project Period (FY) 1989 – 1990
KeywordsOsteoblasts / Bone marrow Cells / Bone Remodeling / Co-Culture / Facs / ALPase Activity / MAC-1 / LFA-1
Research Abstract

The purpose of this investigation was to reveal the physiological bone remodeling mechanism by means of cellular interaction phenomenon between osteoblasts (OB) and bone marrow cells (BM) in vitro.
When OB ( 1X10^6 cells) obtained from 4 weeks old mouse long bones were co-cultivated by using millicell as separator in CO_2 incubator for 14 days, the number of BM was increased and the high incorporation of ^3H-thymidine into cellular DNA of BM was markedly recognized after 3 day incubation. However, the number of BM and uptake of ^3H-thymidine into BM did not increase in BM only cultivation. The increased BM were identified to have surface antigens of Mac-1 and LFA-1 by FACS analysis. But, ALPase activity of OBco-cultivated with BM was remarkably suppressed after 3 days incubation and reduced to one half of that of control after 5 days incubation. These results clearly indicated that both OB and BM produced some differential factors of the clonal growth of Mac-1 and LFA-1 positive cells from BM cells and suppression of ALPase of OB in co-cultivation with OB and BM. It was suggested that the factors produced from interaction between OB and BM might regulate the mechanism of physiological bone remodeling.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 清水 義之,千葉 美麗,清水 義信,三谷 英夫: "Mechanical stressによる骨改造機構に関する研究ー骨改造機構における骨芽細胞と骨髄細胞の相互作用因子についてー" 歯科基礎医学会雑誌. 32. 123-134 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水 義信,奥田 礼一,清水 義之,他: "人工歯根修復に関する模索" 歯科ジャ-ナル. 31. 477-486 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 千葉 美麗,清水 義之,清水 義信,三谷 英夫: "Mechanical stressによる骨改造機構の解明III.破骨細胞の誘導における骨芽細胞の役割と相互作用因子" 日本骨代謝学会雑誌. 8. 216- (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水 義之,千葉 美麗,後藤 安史,清水 義信,熊谷 勝男,三谷 英夫: "骨芽細胞と歯根膜細胞の骨吸収制御機構" 歯科基礎医学会雑誌. 32. 66- (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chiba,M.,Shimizu,Y.,Shimizu,Y.and Mitani,H.: "A study or bone remodeling mechanism induced by mechanical stress IーDifferentiation osteoclasts induced by compressive forcein new bone rat cultured long boneー" J Bone and Mineral Metabolism. 8. 48- (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu,Y.,Chiba,M.,Shimizu,Y.,and Mitani,H.: "A study of bone remodeling mechanism induced mechanical stress IIーFactors of interaction between osteblasts and bone marrow cells in bone remodelingー" J Bone and Mineral Metabolism. 8. 46- (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu, Y., Chiba, M., Shimizu, Y., Mitani, H.: "A study of bone remodeling mechanism induced by mechanical stress -Factors of interaction between osteoblasts and bone marrow cells in bone remodeling" Jpn J Oral Biol. 32. 123-134 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, Y. Shimizu, Y., Okuda, R., Chiba, M., et al.: "Quest for artificial root restoration" J Dent Med. 31. 477-486 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, Y., Chiba, M., Shimizu, Y., Kumagai, K., Mitani, H.: "Study of factors of interaction between osteoblasts and bone marrow cells in bone remodeling" Jpn J Oral Biol. 31S. 171, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chiba, M., Shimizu, Y., Mitani, H.: "Interaction between osteoblasts and bone marrow cells in bone remodeling induced by mechanical stress" J Jpn Orthod Soc. 48. 677 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chiba, M., Shimizu, Y., Simizu, Y., Mitani, H.: "A study of bone remodeling mechanism induced by mechanical stress. 1. Differentiation of osteoclasts induced by compressive force in newborn rat cultured long bone" J Bone Mine Metab. 8. 48 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, Y., Chiba, M., Shimizu, Y., Mitani, H.: "A study of bone remodeling mechanism induced by mechanical stress. 2. Factors of interaction between osteoblasts and bone marrow cells in bone remodeling" J Bone Mine Metab. 8. 46 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chiba, M., Shimizu, Y., Goto, Y., Shimizu, Y., Mitani, H.: "A Study of bone remodeling mechanism induced by mechanical stress. 3. The role of interacting factors between osteoblasts and bone marrow cells in osteoclasts formation" J Bone Mine Metab. 8S. 216 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, Y., Chiba, M., Goto, Y., Shimizu, Y., Kumagai, K., Mitani, H.: "The regulation of bone resorption by osteoblasts and periodontal-ligament cells" Jpn J Oral Biol. 32S. 66 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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