1991 Fiscal Year Final Research Report Summary
Improvement of Gs activity assay, and the application to the screening of receptor disorders.
| Project/Area Number |
01571265
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Institute of Clinical Medicine, University of Tsukuba |
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Principal Investigator |
NAKAI T Institute of Clinical Medicine, Professor, 臨床医学系, 教授 (30049192)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA J Institute of Clinical Medicine, Lecturer, 臨床医学系, 講師 (10110751)
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| Project Period (FY) |
1989 – 1991
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| Keywords | receptor disorder / G-proteins / Gs activity |
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| Research Abstract |
Recent studies have demonstrated the deficiency in the activity of guanine nucleotide regulatory protein(Gs)in patients with pseudohypoparathyroidism. Gs activity has been measured by using cyc^- S49 lymphoma mutant cell line. However, there are some technical difficulties to maintain the cell line in the method. We developed a new method using platelet membrane in place of the cultured cell membrane in the previous method.
We have shown that many patients with pseudohypoparathyroidism and alcoholism manifest reduced erythrocyte Gs activity using this method. We found that this method is convenient and useful for not only the screening of the receptor disorder(Gs deficiency)but also discrimination of, -the patients with alcoholism from normal subjects.
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