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1990 Fiscal Year Final Research Report Summary

Analysis of Oncogene Amplification and C-Myc Overexpression in Human Ovarian Cancer

Research Project

Project/Area Number 01571283
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKyoto University

Principal Investigator

FUKUMOTO Manabu  Fac. Medicine, Dept. Pathology. Instructor, 医学部, 助手 (60156809)

Project Period (FY) 1989 – 1990
KeywordsHuman Tumor / Ovarian Cancer / Oncogene / Gene Amplification / Gene Expression / Tumor Tissue / C-myc
Research Abstract

Amplification and expression of oncogenes were investigated in 55 cases of human ovarian cancer tissues. Gene amplificaion of unknown nature was screened by in-gel renaturation assay and amplification of the c-myc, Ki-ras and neu oncogenes were determined by Southern blot hybridization. No significant gene amplification was found in all the cases. By Northern hybridization assay, the c-myc gene was overexpressed in 29 cases (52.7%) whereas either k-ras or neu was not.
The overexpression more than 3-fold of the c-myc gene was significant in serous cystadenocarcinoma and endometrioid carcinoma tissues compared with other histological types of tumor. Immunohistochemistry and in-situ hybridization revealed that tumor cells overexpressed both the c-myc gene and the protein.
Although the expression level was not related to grading of malignancy, it is suggested that the c-myc gene is involved in early stages of carcinogesis of ovarian tumors.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Fukumoto,M.et al.: "Association of Kiーras with Amplified DNA Sequences,Detection in Human Ovarian Carcinoma by a Modified Inーgel Renaturation Assay." Cancer Res.49. 1693-1697 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukumoto,M.et al.: "Analysis of Gene Amplification in Human Tumor Cells by Inーgel DNA Renaturation Assay." Acta Heamatol.Jpn.52. 1463-1470 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福本 学: "Inーgel DNA renaturation technique:遺伝子増幅の一般的検索法と腫瘍における遺伝子増幅" 日本産科婦人科学会熊本地方部会誌. 33. 71-77 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福本 学: "癌化における遺伝子増幅" Medical Immunology. 18. 147-155 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福本 学: "増幅遺伝子はなぜ増える" オンコロジア. 23. 134-136 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福本 学: "発癌の分子遺伝学" 日本産科婦人科熊本地方部会誌. 34. 93-99 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukumoto,M.& Midorikawa,O.: "Molecular Biology of Cancer Genes" ed.by Sluyser,M.(Ellis Horwood Ltd.,London), (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukumoto, M. et al.: "Association of Ki-ras with Amplified DNA Sequences, Detection in Human Ovarian Carcinomas by a Modified In-gel Renaturation Assay." Cancer Res. 49. 1693-1697 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukumoto, M. et al.: "Analysis of Gene Amplification in Human Tumor Cells by In-gel DNA Renaturation Assay." Acta Haematol. Jpn.52(8). 1463-1470 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukumoto, M.: "Gene Amplification in Carcinogenesis." Med. Immunol.18(4). 147-155 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukumoto, M.: "Why Amplified Genes are Amplified?" Oncologia. 23(3). 134-136 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukumoto, M. et al.: "Analysis of c-myc Amplification and Expression in Xenotransplanted Human Prostatic Carcinoma Cell." Molecula Biology of Cancer Genes. (ed. by Sluyser, M.). Ellis Horwood Ltd. London. 193-206 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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