1990 Fiscal Year Final Research Report Summary
Analysis of Oncogene Amplification and C-Myc Overexpression in Human Ovarian Cancer
| Project/Area Number |
01571283
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Kyoto University |
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Principal Investigator |
FUKUMOTO Manabu Fac. Medicine, Dept. Pathology. Instructor, 医学部, 助手 (60156809)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Human Tumor / Ovarian Cancer / Oncogene / Gene Amplification / Gene Expression / Tumor Tissue / C-myc |
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| Research Abstract |
Amplification and expression of oncogenes were investigated in 55 cases of human ovarian cancer tissues. Gene amplificaion of unknown nature was screened by in-gel renaturation assay and amplification of the c-myc, Ki-ras and neu oncogenes were determined by Southern blot hybridization. No significant gene amplification was found in all the cases. By Northern hybridization assay, the c-myc gene was overexpressed in 29 cases (52.7%) whereas either k-ras or neu was not.
The overexpression more than 3-fold of the c-myc gene was significant in serous cystadenocarcinoma and endometrioid carcinoma tissues compared with other histological types of tumor. Immunohistochemistry and in-situ hybridization revealed that tumor cells overexpressed both the c-myc gene and the protein.
Although the expression level was not related to grading of malignancy, it is suggested that the c-myc gene is involved in early stages of carcinogesis of ovarian tumors.
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