1990 Fiscal Year Final Research Report Summary
Structure and Expression of Drosophila Chat Gene and its Related Gene
| Project/Area Number |
01580176
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Kyoto University |
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Principal Investigator |
ITOH Nobuyuki Kyoto University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (10110610)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Neurotransmitter / Gene / Drosophila |
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| Research Abstract |
Choline acetyltransferase is the biosynthetic enzyme for the neurotransmitter acetylcholine. By screening Drosophila cDNA library with a mixture of three different monoclonal antibodies to Drosophila choline acetyltransferase, we isolated Drosophila choline acetyltransferase cDNA. The cDNA insert consisted of a coding region 2,190 nucleotides long and a 3' -noncoding region 284 nucleotides long. Northern analysis of Drosophila head mRNA with the cDNA insert as a probe showed the choline acetyltransferase mRNA to be approximately 4,700 nucleotides long. We compared the amino acid sequences of Drosophila choline acetyltransferase and Torpedo acetylcholinesterase and found a global homology of 21-44% identity between these proteins. Six homologous segments of 40-60 amino acids cover 38% and 54% of the sequences, raising the possibility of a common evolutionary origin. We also isolated and characterized the cDNA related with Drosophia choline acetyltransferase.
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