• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1990 Fiscal Year Final Research Report Summary

Electron Carrier System and Energy Metabolism in Anaerobic Microorganisms

Research Project

Project/Area Number 01580187
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionShizuoka University

Principal Investigator

YAGI Tatsuhiko  Shizuoka University, Faculty of Education, Professor, 教育学部, 教授 (00021882)

Project Period (FY) 1989 – 1990
KeywordsSulfate-reducing bacterium / Electron carrier protein / High-molecular-weight cytochrome / Hemoprotein / Rubredoxin / Amino acid sequence / Anaerobic syntheses of heme / Cytochrome c-553
Research Abstract

Effort was focused on the elucidation of the high-molecularーweight cytochrome c (hmc) among various electron carrier proteins isolated from Desulfovibrio vulgaris Miyazaki.
1. The hmc was highly purified in good yield, by eliminating the steps where the hmc was exposed to proteolytic digestion. It had been realized that the hmc was extremely susceptible to the action of proteinases present in the bacterial extract.
2. Quantitative estimation of amino acids indicated that the hmc contained 12 hemes in a single peptide of Mr : 67000.
3. The spectral properties of the hmc was similar to those of cytochrome C_3. The purity index (the alpha-peak height of the ferro-form divided by the absorbance at 280 nm) was 2.6. There was no peak at 695 nm which is a marker for the His-Met axial ligands for hemes. The reduced-minus-oxidized difference spectrum of the fully reduced hmc had a peak at 420 nm, whereas the difference spectra of partially reduced forms had double peaks at 424 and 434 nm. This indicates conformational transformation or the appearance of the high-spin form during the reduction of the hmc.
4. One of 12 hemes of hmc had a relatively positive standard redox potential, and is reduced with ascorbate. The standard redox potentials of the other hemes were estimated by redox equilibrium with FMN and calculated by means of the Nernst equation. The average E^゚' value was -187 mV with an appearent n valve of 0.48 (i. e., less than unity). The redox potentials of the individual hemes were calculated from these values.
5. Partial sequences of the hmc were determined, which were homologous to those of hmc from the Hildenborough strain of D. vulgaris.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 田坂 千草: "Desulpovibrio vulgaris Miyazakiに存在する高分子型シトクロムC^3の構造と物性" 第41回 タンパク質構造討論会 要旨集. 41. 49-52 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 八木 達彦: "硫酸還元菌Desulfovibrio vulgaris Miyazaki株の膜結合型ヒドロゲナ-ゼの改良精製法" 豊田研究報告. 43. 27-31 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Nakagawa: "Sーclass cytochromes c have a variety of folding patterns: Structure of cytochrome cー553 from Desulfovibrio vulgaris determined by the multiーwavelength anomalous dispersion method" The Journal of Biochemistry. 108. 701-703 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Tasaka: "Partial sequences of highーmolecularーweight cytochrome c isolated from Desulfovibrio vulgaris Miyazaki" Protein Sequences & Data Analysis. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 八木 達彦: "Desulfovibrio vnlgaris Miyazakiの可溶性ヒドロゲナ-ゼの精製と物性" 豊田研究報告. 44. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yagi: "Microbiology and Biochemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer(J.ーP.Belaich,M.Bruschi,and J.ーL.Garcia,EdS.),Part 2,Section 2ー1.Microbiology Electron Carrier proteins in Desulfovibrio vulgaris Miyazaki" Plenum Pubishing Corporation,New York, (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C. Tasaka, M. Ogata, T. Yagi, and A. Tsugita: "Structure and properties of high-molecular-weight cytochrome C_3 in Desulfovibrio vulgaris." Tanpakushitsu Kozo Toronkai Abstract of Papers. No. 41. 49-52 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yagi: "Improved procedure for the purification of membranebound hydrogenase from Desulfovibrio vulgaris Miyazaki." Reports of Toyoda Physical and Chemical Research Institute. vol. 43. 27-31 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Nakagawa, Y. Higuchi, N. Yasuoka, Y. Katsube, and T. Yagi: "S-class cytochromes c have a variety of folding patterns : Structure of cytochrome c-553 from Desulfovibrio vulgaris dertermined by the multi-wavelength anomalous dispersion method." Journal of Biochemistry. 108. 701-703 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C. Tasaka, M. Ogata, T. Yagi, and A. Tsugita: "Partial sequences of high-molecular-weight cytochrome c isolated from Desulfovibrio vulgaris Miyazaki." Protein Sequences & Data Analysis. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yagi, K. Takahashi, and M. Ogata: "Purification and properties of soluble hydrogenase from Desulfovibrio vulgaris Miyazaki." Reports of Toyoda Physical and Chemical Research Institute. vol. 44. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yagi: "Electron carrier Proteins in Desulfovibrio vulgaris Miyazaki." Plenum Publishing Corporation, N. Y. "Microbiology and Biochemistry of Strict Anaerobes Involvd in Interspecies Hydrogen Transfer" (J. -P. Belaich, M. Bruschi, and J. -L. Garcia, Eds.). 237-248

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-08-12  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi