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1991 Fiscal Year Final Research Report Summary

Purification and biochemical, cell biological analysis o a new human GTP-binding protein

Research Project

Project/Area Number 01580199
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionKeio university School of Medicine

Principal Investigator

TAKANO Toshiya  Keio University School of Medicine, Department of Microbiology, Professor, 医学部, 教授 (60051364)

Co-Investigator(Kenkyū-buntansha) TACHIBANA Kouichi  Keio University School of Medicine, Department of Microbiology, Instructor, 医学部, 助手 (80216986)
Project Period (FY) 1989 – 1991
KeywordsRab-2 gene / GTP-binding protein / Transcription / Complementary DNA / Messenger RNA / Polyadenylation site / Antiserum / Modification of protein
Research Abstract

The human rab-2 gene codes a 24-kDa YPT1-related GTP-binding protein. In all the human cell lines examined, including hematopoietic, fibroblastic and tumor cells, three rab-2 transcripts of 3.5, 2.4 and 1.4 kb were detected. The 2.4- and 3.5-kb rab-2 RNA hybridized with the probe specific for the 3'-non-coding region of 2.4-kb transcript, but not the 1.4-kb RNA. These transcripts seemed to differ in the length of their 3'-non-translated regions due to terminations of the transcription and/or processing of the primary transcript at the alternative polyadenylation sites. The smaller 1.4-kb transcript was the most predominant in Molt-4 and U-937, while the 2.4-kb RNA was dominant in the other cell lines. The degradation rates of all the rab-2 transcripts wbre similarly as slow as beta-actin RNA, even in the two cell lines whose predominant species of the transcripts were different. Rab-2 protein was detected in the extracts of human cultured cells as one major band of 24 kDa and a more faint band of 25 kDa by both immunoblotting and immunoprecipitation. Only the 25-kDa protein was detected in HeLa and KOBK101 cells. In the COS-1 cells transfected with an expression vector carrying rab-2 cDNA, the rab-2 protein was mainly located in the cytoplasm.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Kato,S.et al.: "The entire nucleotide sequence of baboon endogenous virus DNA;a chimeric genome structure of murine type C and simian type D retroviruses." Jpn.J.Genet.62. 127-137 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tachibana,K.et al.: "Nucleotide sequence of a new YPT1-related human cDNA which belongs to the ras gene superfamily." Nucl.Acids Res.16. 10368-10368 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ko,M.S.H.et al.: "A highly inducible system of gene expression by positive feedback production of glucocorticoid receptors." DNA. 8. 127-133 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ko,M.S.H.et al.: "An auto-inducible vector conferring high glucocorticoid inducibility upon stable transformant cells." Gene. 84. 383-389 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ko,M.S.H.et al.: "Genetic recombination in a chromosomal translocation t(2;9)(p11;q24)of as Burkitt's lymphoma cell line,KOBK101." Gene. 97. 239-244 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Umezawa,A.et al.: "Colony-stimulating factor 1 expression is down-regulated during the adipocyte differentiation of H-1/A marrow stromal cells and induced by cachectin/tumor necrosis factor." Mol.Cell.Biol.11. 920-927 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tachibana, K., Umezawa, A. and Kato, S. and Takano, T.: "Nucleotide sequence of a new YPT1-related human cDNA which belongs to the ras gene superfamily." Nucleic Acid Research. 16. 10368 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tachibana, K., Umezawa, A. and Takano, T.: "Transcripts and proteins coded by human rab-2 gene."

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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