1989 Fiscal Year Annual Research Report

細胞生産装置の複製

Research Project

Project/Area Number	01615006
Research Institution	National Institute of Genetics
Principal Investigator	石浜明国立遺伝学研究所, 分子遺伝研究系, 教授 (80019869)
Co-Investigator(Kenkyū-buntansha)	西村暹国立がんセンター研究所, 生物物理部, 部長 (20076970) 福田龍二金沢大学, 医学部, 教授 (60027331) 磯野克己神戸大学, 理学部, 教授 (70011640) 中村義一東京大学, 医科学研究所, 助教授 (40114590) 由良隆京都大学, ウイルス研究所, 教授 (20027311)
Keywords	転写制御 / RNAポリメラ-ゼ / 転写因子 / リボゾ-ム / 翻訳制御 / tRNA / 大腸菌 / ストレス応答
Research Abstract	細胞複製に連動した、遺伝子発現の包括的制御を分子の水準で解明することを目標として、細胞生産装置なかでも転写装置と翻訳装置の形成と機能制御の解析を進めてきた。本年度は、下記の成果が得られた。 1.大腸菌染色体に存在するすべてのプロモ-タ-の同定、強度順位の決定とサイクリックAMPなどの転写因子に対する感変性を指標とした分類を進め、転写シグナルの全体像にさらに接近した。 2.転写装置の基幹成分RNAポリメラ-ゼ各サブユニットの分担機能、サブユニット蛋白上の機能ドメインを同定する研究を引続き進め、機能地図の概要を作成した。 3.熱ショックなどのストレスを加えた時のRNAポリメラ-ゼの機能変化の構造基盤を明らかにしたのに続いて、複合要因による増殖静止期への移行時には、転写装置・翻訳装置が貯蔵型に変換することを発見した。 4.RNAポリメラ-ゼの機能制御に転写因子との相互作用があることを先に実証したが、転写因子の活性調節に蛋白リン酸化が関与することを発見した。 5.翻訳装置リボゾ-ムの機能制御に関与する「リボゾ-ム修飾因子」(RMF)を発見した。また、リボゾ-ム蛋白の修飾に関与する遺伝子の生理機能の探索を続けた。酵母ミトコンドリア・リボゾ-ム蛋白の遺伝子12種を単離し構造を決定した。 6.マイコプラズマの全tRNA遺伝子を同定した。また、真核生物の天然サプレッサ-tRNAを発見し、翻訳制御の新しい機構を明らかにした。

Research Products

(22 results)

All Other

All Publications (22 results)

[Publications] Igarashi,K.: "Promoter selectivity of Escherichia coli RNA polymerase:Omega factor is responsible for the ppGpp sensitivity." Nucleic Acids Res.17. 8755-8765 (1989)
[Publications] Nakayama,M.: "Microcollus luteus,a bacterium with a high G+C content,contains Escherichia coliーtype promoters." Mol.Gen.Genet.218. 384-389 (1989)
[Publications] Wada,A.: "Structure and probable genetic map of a ribosome modulation factor associated with 100S ribosome in stationaryーphase Escherichia coli." Proc.Natl.Acad.Sci.USA. 87. (1990)
[Publications] Aiba,H.: "Semisynthetic promoters activated by cyclic AMP receptor protein of Escherichia coli." Gene. 85. 91-97 (1989)
[Publications] Kihara,M.: "Flagella switch of Salmonella typhmurium:Gene Sequences and deduced protein sequences." J.Bacteriol.171. 3247-3257 (1989)
[Publications] Inada,T.: "Temperatuerーsensitive lethal mutant of Era,a G protein in Escherichia coli." J.Bacteriol.171. 5017-5024 (1989)
[Publications] Aiba,H.: "Transfer of phosphoryla group between two regulatory proteins involved in osmoregulatory expression of the ompF and ompC genes." J.Biol.Chem.264. 8563-8567 (1989)
[Publications] Aiba,H.: "Evidence for the physilogical importance of the phosphotransfer between the two regulatory components,EnvZ and OmpR,in osmoregulation in Escherichia coli." J.Biol.Chem.264. 14090-14094 (1989)
[Publications] Wada,M.: "Participation of the hup gene product in siteーspecific DNA inversion in Escherichia coli." Gene. 76. 345-352 (1989)
[Publications] Tanaka,S.: "Cloning and molecular characterization of the gene rimL which encodes an enzyme acetylating ribosomal protein L12 of Escherichia coli." Mol.Gen.Genet.217. 289-293 (1989)
[Publications] Matsushita,Y.: "Cloning and analysis of the nuclear genes for two mitochondrial ribosomal proteins in yeast." Mol.Gen.Genet.
[Publications] Adachi,Y.: "Codon recognition patterns as deduced from sequences of the complete set of transfer RNA species in Micoplasma capricolum." J.Mol.Biol. 209. 37-54 (1989)
[Publications] Tani,T.: "The gene for the U6 small nuclear RNA in fission yeast has an intron." Nature. 337. 87-90 (1989)
[Publications] Ito,M.: "Independent removal of two identical or different introns from mRNA precursors in vitro." J.Biohem.105. 1014-1023 (1989)
[Publications] Fujioka,M.: "Purification of an autocrine growth factor in conditioned medium obtained from primary cultures of scleral fibroblasts of the chich embryo." Exp.Cell Res.181. 400-408 (1989)
[Publications] Bardwell,J.C.A.: "RNase III operon expression is autogenously regulated by mRNA processing." EMBO J.
[Publications] Serizawa,H.: "Analysis of the promoter of the gene for an RNA polymeraseーbinding protein." Mol.Gen.Genet.
[Publications] Igarashi,K.: "Functional mapping of the alpha subunit of Escherichia coli RNA polymerase." J.Mol.Biol.
[Publications] Ishihama,A.: "Adv.Biophys.,Vol.26" Jpn.Sci.Soc.Press/Elsevier, (1990)
[Publications] Ishihama,A.: "Structure and Function of Nucleic Acides and Proteins" Raven Press,New York, (1990)
[Publications] 石浜明: "シリ-ズ分子生物学の進歩,第4巻「遺伝子の発現と制御」" 丸善,東京, 296 (1989)
[Publications] 石浜明: "分子生物科学,第2巻「遺伝子と遺伝の情報」" 岩波書店,東京, 154 (1989)

1989 Fiscal Year Annual Research Report

細胞生産装置の複製

Principal Investigator

石浜 明 国立遺伝学研究所, 分子遺伝研究系, 教授 (80019869)

Research Products

[Publications] Igarashi,K.: "Promoter selectivity of Escherichia coli RNA polymerase:Omega factor is responsible for the ppGpp sensitivity." Nucleic Acids Res.17. 8755-8765 (1989)

[Publications] Nakayama,M.: "Microcollus luteus,a bacterium with a high G+C content,contains Escherichia coliーtype promoters." Mol.Gen.Genet.218. 384-389 (1989)

[Publications] Wada,A.: "Structure and probable genetic map of a ribosome modulation factor associated with 100S ribosome in stationaryーphase Escherichia coli." Proc.Natl.Acad.Sci.USA. 87. (1990)

[Publications] Aiba,H.: "Semisynthetic promoters activated by cyclic AMP receptor protein of Escherichia coli." Gene. 85. 91-97 (1989)

[Publications] Kihara,M.: "Flagella switch of Salmonella typhmurium:Gene Sequences and deduced protein sequences." J.Bacteriol.171. 3247-3257 (1989)

[Publications] Inada,T.: "Temperatuerーsensitive lethal mutant of Era,a G protein in Escherichia coli." J.Bacteriol.171. 5017-5024 (1989)

[Publications] Aiba,H.: "Transfer of phosphoryla group between two regulatory proteins involved in osmoregulatory expression of the ompF and ompC genes." J.Biol.Chem.264. 8563-8567 (1989)

[Publications] Aiba,H.: "Evidence for the physilogical importance of the phosphotransfer between the two regulatory components,EnvZ and OmpR,in osmoregulation in Escherichia coli." J.Biol.Chem.264. 14090-14094 (1989)

[Publications] Wada,M.: "Participation of the hup gene product in siteーspecific DNA inversion in Escherichia coli." Gene. 76. 345-352 (1989)

[Publications] Tanaka,S.: "Cloning and molecular characterization of the gene rimL which encodes an enzyme acetylating ribosomal protein L12 of Escherichia coli." Mol.Gen.Genet.217. 289-293 (1989)

[Publications] Matsushita,Y.: "Cloning and analysis of the nuclear genes for two mitochondrial ribosomal proteins in yeast." Mol.Gen.Genet.

[Publications] Adachi,Y.: "Codon recognition patterns as deduced from sequences of the complete set of transfer RNA species in Micoplasma capricolum." J.Mol.Biol. 209. 37-54 (1989)

[Publications] Tani,T.: "The gene for the U6 small nuclear RNA in fission yeast has an intron." Nature. 337. 87-90 (1989)

[Publications] Ito,M.: "Independent removal of two identical or different introns from mRNA precursors in vitro." J.Biohem.105. 1014-1023 (1989)

[Publications] Fujioka,M.: "Purification of an autocrine growth factor in conditioned medium obtained from primary cultures of scleral fibroblasts of the chich embryo." Exp.Cell Res.181. 400-408 (1989)

[Publications] Bardwell,J.C.A.: "RNase III operon expression is autogenously regulated by mRNA processing." EMBO J.

[Publications] Serizawa,H.: "Analysis of the promoter of the gene for an RNA polymeraseーbinding protein." Mol.Gen.Genet.

[Publications] Igarashi,K.: "Functional mapping of the alpha subunit of Escherichia coli RNA polymerase." J.Mol.Biol.

[Publications] Ishihama,A.: "Adv.Biophys.,Vol.26" Jpn.Sci.Soc.Press/Elsevier, (1990)

[Publications] Ishihama,A.: "Structure and Function of Nucleic Acides and Proteins" Raven Press,New York, (1990)

[Publications] 石浜明: "シリ-ズ分子生物学の進歩,第4巻「遺伝子の発現と制御」" 丸善,東京, 296 (1989)

[Publications] 石浜明: "分子生物科学,第2巻「遺伝子と遺伝の情報」" 岩波書店,東京, 154 (1989)

石浜明国立遺伝学研究所, 分子遺伝研究系, 教授 (80019869)