1989 Fiscal Year Annual Research Report

全能性発現の分子機構

Research Project

Project/Area Number	01622003
Research Institution	Nagoya University
Principal Investigator	渡辺昭名古屋大学, 農学部, 教授 (70023471)
Co-Investigator(Kenkyū-buntansha)	高橋陽介名古屋大学, 理学部, 助手 (90183855) 長田敏行岡崎国立共同研究機構, 基礎生物学研究所, 助学授 (10012519) 南川隆雄東京都立大学, 理学部, 教授 (30087001) 庄野邦彦東京大学, 教養学部, 教授 (60050457) 駒嶺穆東北大学, 理学部, 教授 (90011494)
Keywords	全能性 / 脱分化 / 遺伝子導入植物 / Agrobacterium rhizogenes / タバコ / 遺伝的腫瘍形成 / トランスジェニック植物 / オーキシン
Research Abstract	1.タバコ葉肉細胞が脱分化を起こす時に特異的に発現する遺伝子を単離することに成功した。この遺伝子は、G0期にある葉肉細胞のプロトプラストに脱分化を促す植物ホルモンであるオーキシンを与えると、10分で発現が検出され、4時間以降24時間まで強い発現が見られるが、細胞がS期に入る前に停止するという一過性の発現様式を示す。この遺伝子の構造解析から、大腸菌の飢餓条件で発現する遺伝子と相同性を持つことが判明し、RNAポリメラーゼと相互作用を持つことにより、調節遺伝子である可能性が示唆された。この遺伝子をタバコに導入してトランスジェニック植物を作製し、植物個体内で恒常的に過剰発現されたところ、オーキシンによって保たれる頂芽優勢が破れ、脇芽の発育が旺盛になった。これらの観察は、この遺伝子の産物は、脱分化のみではなく、より一般的にオーキシン作用の情報伝達機構の一部に関与していることを示唆している。 2.Agrobacterium rhizogenesのRiプラスミドには、植物体で発現するrolA〜Dとよばれる遺伝子が存在する。この遺伝子を植物に導入すると植物体が矮化すること、また、維管束細胞に特異的に発現すること、などがすでに本重点領域研究の研究によって明らかにされていた。一方、タバコ属の中の一群の種の染色体には、偽遺伝子と考えられていたrolBおよびCと相同的な配列(Ngrol)が存在することが知られていた。本年度の研究によって、この配列を持つ種(N.glauca)と持たない種(N.langsdorfii)の異種かけあわせによって生じる遺伝的腫瘍形成の過程を調べたところ、これらの配列が遺伝子として発現していることが発見された。この結果は、タバコ属の種の進化の過程でこれらの遺伝子が植物に移行し、細胞の脱分化、および器官形成に働いてきた可能性を強く示唆するものである。

Research Products
(35 results)

All Other

All Publications (35 results)

[Publications] Akasofu,H.: "Nucleotide sequence of cDNA for sulfhydryl-endopeptidase(SH-EP)from cotyledons of germinating Vigna mungo seeds" Nucleic Acids Res.17. 6733 (1989)
[Publications] Ishibashi,N.: "Molecular cloning and characterization of stored mRNA in cotyledons of Vigna unguiculata seeds" Plant Cell Physiol.30. (1990)
[Publications] Yamauchi,D.: "Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells" FEBS Lett.(1990)
[Publications] H.Morikawa: "Construction of holding pipettes for microinjection." Plant Tissue Culture Letters.6. 98-100 (1989)
[Publications] H.Morikawa: "Transient expression of foreign genes in plant cells and tissues obtained by a simple biolistic device(particlegun)." Appl.Mocrobiol.Biotechnol.31. 320-322 (1989)
[Publications] A.Iida: "Culture of isolated tobacco mesophyll cells." Plant Tissue Culture Letters. 6. 169-171 (1989)
[Publications] Y.Morohashi: "Control of -amylase development in cotyledons during and following germination of mung bean seeds" Plant Physiology. 91. 253-258 (1989)
[Publications] M.Sugimoto: "Comparison of the developmental patterns of mitochondria malate dehydrogenase between mung bean and cucumber cotyledons during and following germination" Physiologia Plantarum. 77. 238-243 (1989)
[Publications] Kamata,Y.: "Acceleration of chloroplast division in somatic cell hybrids between mesophyll and cell culture protoplasts." Plant Cell Physiol.30. 151-156 (1989)
[Publications] K.Ozawa: "Establishment of a system of high-frequency embryo-genesis from long-term cell suspension culture of rice." Theor Appl Genet. 77. 205-211 (1989)
[Publications] S.Hirose: "Changes in ultrastructure of Golgi apparatus during the cell cycle in a synchronous culture of Catharanthus roseus." New Phytologist. 111. 599-605 (1989)
[Publications] H.Kodama: "Phase-Specific Polypeptides and Ploy(A)^+ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells." Plant Physiol.89. 910-917 (1989)
[Publications] A.Komamine: "High frequency and synchronous somatic embryogenesis,a useful system for crop improvement." Food and Fertilizer Technology Center,Technical Bulletin. 113. 1-4 (1989)
[Publications] S.Amino: "Changes in the wall-bound glycosidase activities curing the cell cycle in a synchronous culture of Catharanthus roseus" Z.Naturforschung. 44. 754 (1989)
[Publications] Yamauchi,D.: "Nucleotide sequence of cDNA for concanavalin A from Canavalia gladiata seeds" Plant Cell Physiol.30. 147-150 (1989)
[Publications] Ishibashi,N.: "Cotyledonary mRNA species and germinability of immature Vigna unguiculata seeds" Plant Cell Physiol.30. 373-379 (1989)
[Publications] Mitsuhashi,W.: "Synthesis and posttranslational activation of sulfhydryl-endopeptidase incotyledons of germinating Vigna mungo seeds" Plant Physiol.89. 274-279 (1989)
[Publications] Takei,Y.: "Nucleotide sequence of the canavalin gene from Canavalia gladiata seeds" Nucleic Acids Res.17. 4381 (1989)
[Publications] Nagata,T.: "Cell biological aspects of gene delivery into plant protoplasts by electroporation." Internatl.Rev.Cytol.116. 229-255 (1989)
[Publications] Takahashi,Y.: "Isolation of an auxin-regulated gene cDNA expressed during the transition from G_0 to S phase in tobacco mesophyll protoplasts." Proc.Natl.Acad.Sci.USA. 86. 9279-9283 (1989)
[Publications] Ozeki,Y.: "Induction and repression of phenylalanine ammonia-lyase and chalcone synthase enzyme proteins and mRNAs in carrot suspension cultures regulated by 2,4-D." Physiologia Plantarum. (1990)
[Publications] Takeda,S.: "Photosynthetic characteristics in photoautotrophically cultured cells of tobacco" Plant & Cell physiology. 30. 885-891 (1989)
[Publications] Takeda,S.: "Characterization of polypeptides that accumulate in cultured Nicotiana tabacum cells" Plant & Cell Physiology.31. (1990)
[Publications] Kikuchi Motoyuki: "Modulation of gene expression in isolated nuclei by auxin-binding proteins" Plant Cell Physiol.30. 765-773 (1989)
[Publications] Takanari Ichikawa: "Tumorization and redifferentiation system of tobacco genetic tumor" Plant Cell Physiol.29. 1373-1378 (1988)
[Publications] Takanari Ichikawa: "Morphological observation and qualitative and quantitative studies of auxins after induction of tobacco genetic tumors" Plant Cell Physiol.30. 57-63 (1989)
[Publications] Takanari Ichikawa: "Evidence for the expression of the rol genes of Nicotiana glauca in genetic tumor of Nicotiana glauca x N__ー.langsdorffii" Mol.Gen.Genet.220. 177-180 (1990)
[Publications] Fukuda,H.: "Cytodifferentiation in isolated single cells." Bot.Mag.Tokyo. 102. 111-121 (1989)
[Publications] Sugiyama,M.,Fukuda,H.,Komamine A.: "Characteristics of the inhibitory effect of 5-fluorodeoxyuridine on cytodifferentiation into tracheary elements of isolated mesophyll cells of Zinnia elegans." Plant Cell Physiol.31. 61-67 (1990)
[Publications] Fukuda H.: "Regulation of tubulin degradation in isolated Zinnia mesophyll cells in culture." Plant Cell Physiol.30. 243-252 (1989)
[Publications] Fukuda,H.,Kobayashi,H.: "Dynamic organization of the cytoskeleton during tracheary-element differentiation." Develop.Growth Differ.31. 9-16 (1989)
[Publications] 福田裕穂: "細胞分化と細胞骨格" 細胞. 21. 306-310 (1989)
[Publications] 西村いくこ: "オルガネラの形成ー単膜系オルガネラ、“現代植物生理学第3巻成長と分化"柴岡弘郎編" 朝倉書店, (1990)
[Publications] Akira Watanabe: "Cell Separation in Plants" Springer-Verlag, 31-38 (1989)
[Publications] 鎌田博: "植物バイオテクノロジー事典" 日本エンタープライズ社(東京), 235 (1989)

1989 Fiscal Year Annual Research Report

全能性発現の分子機構

Principal Investigator

渡辺 昭 名古屋大学, 農学部, 教授 (70023471)

Research Products

[Publications] Akasofu,H.: "Nucleotide sequence of cDNA for sulfhydryl-endopeptidase(SH-EP)from cotyledons of germinating Vigna mungo seeds" Nucleic Acids Res.17. 6733 (1989)

[Publications] Ishibashi,N.: "Molecular cloning and characterization of stored mRNA in cotyledons of Vigna unguiculata seeds" Plant Cell Physiol.30. (1990)

[Publications] Yamauchi,D.: "Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells" FEBS Lett.(1990)

[Publications] H.Morikawa: "Construction of holding pipettes for microinjection." Plant Tissue Culture Letters.6. 98-100 (1989)

[Publications] H.Morikawa: "Transient expression of foreign genes in plant cells and tissues obtained by a simple biolistic device(particlegun)." Appl.Mocrobiol.Biotechnol.31. 320-322 (1989)

[Publications] A.Iida: "Culture of isolated tobacco mesophyll cells." Plant Tissue Culture Letters. 6. 169-171 (1989)

[Publications] Y.Morohashi: "Control of -amylase development in cotyledons during and following germination of mung bean seeds" Plant Physiology. 91. 253-258 (1989)

[Publications] M.Sugimoto: "Comparison of the developmental patterns of mitochondria malate dehydrogenase between mung bean and cucumber cotyledons during and following germination" Physiologia Plantarum. 77. 238-243 (1989)

[Publications] Kamata,Y.: "Acceleration of chloroplast division in somatic cell hybrids between mesophyll and cell culture protoplasts." Plant Cell Physiol.30. 151-156 (1989)

[Publications] K.Ozawa: "Establishment of a system of high-frequency embryo-genesis from long-term cell suspension culture of rice." Theor Appl Genet. 77. 205-211 (1989)

[Publications] S.Hirose: "Changes in ultrastructure of Golgi apparatus during the cell cycle in a synchronous culture of Catharanthus roseus." New Phytologist. 111. 599-605 (1989)

[Publications] H.Kodama: "Phase-Specific Polypeptides and Ploy(A)^+ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells." Plant Physiol.89. 910-917 (1989)

[Publications] A.Komamine: "High frequency and synchronous somatic embryogenesis,a useful system for crop improvement." Food and Fertilizer Technology Center,Technical Bulletin. 113. 1-4 (1989)

[Publications] S.Amino: "Changes in the wall-bound glycosidase activities curing the cell cycle in a synchronous culture of Catharanthus roseus" Z.Naturforschung. 44. 754 (1989)

[Publications] Yamauchi,D.: "Nucleotide sequence of cDNA for concanavalin A from Canavalia gladiata seeds" Plant Cell Physiol.30. 147-150 (1989)

[Publications] Ishibashi,N.: "Cotyledonary mRNA species and germinability of immature Vigna unguiculata seeds" Plant Cell Physiol.30. 373-379 (1989)

[Publications] Mitsuhashi,W.: "Synthesis and posttranslational activation of sulfhydryl-endopeptidase incotyledons of germinating Vigna mungo seeds" Plant Physiol.89. 274-279 (1989)

[Publications] Takei,Y.: "Nucleotide sequence of the canavalin gene from Canavalia gladiata seeds" Nucleic Acids Res.17. 4381 (1989)

[Publications] Nagata,T.: "Cell biological aspects of gene delivery into plant protoplasts by electroporation." Internatl.Rev.Cytol.116. 229-255 (1989)

[Publications] Takahashi,Y.: "Isolation of an auxin-regulated gene cDNA expressed during the transition from G_0 to S phase in tobacco mesophyll protoplasts." Proc.Natl.Acad.Sci.USA. 86. 9279-9283 (1989)

[Publications] Ozeki,Y.: "Induction and repression of phenylalanine ammonia-lyase and chalcone synthase enzyme proteins and mRNAs in carrot suspension cultures regulated by 2,4-D." Physiologia Plantarum. (1990)

[Publications] Takeda,S.: "Photosynthetic characteristics in photoautotrophically cultured cells of tobacco" Plant & Cell physiology. 30. 885-891 (1989)

[Publications] Takeda,S.: "Characterization of polypeptides that accumulate in cultured Nicotiana tabacum cells" Plant & Cell Physiology.31. (1990)

[Publications] Kikuchi Motoyuki: "Modulation of gene expression in isolated nuclei by auxin-binding proteins" Plant Cell Physiol.30. 765-773 (1989)

[Publications] Takanari Ichikawa: "Tumorization and redifferentiation system of tobacco genetic tumor" Plant Cell Physiol.29. 1373-1378 (1988)

[Publications] Takanari Ichikawa: "Morphological observation and qualitative and quantitative studies of auxins after induction of tobacco genetic tumors" Plant Cell Physiol.30. 57-63 (1989)

[Publications] Takanari Ichikawa: "Evidence for the expression of the rol genes of Nicotiana glauca in genetic tumor of Nicotiana glauca x N__ー.langsdorffii" Mol.Gen.Genet.220. 177-180 (1990)

[Publications] Fukuda,H.: "Cytodifferentiation in isolated single cells." Bot.Mag.Tokyo. 102. 111-121 (1989)

[Publications] Sugiyama,M.,Fukuda,H.,Komamine A.: "Characteristics of the inhibitory effect of 5-fluorodeoxyuridine on cytodifferentiation into tracheary elements of isolated mesophyll cells of Zinnia elegans." Plant Cell Physiol.31. 61-67 (1990)

[Publications] Fukuda H.: "Regulation of tubulin degradation in isolated Zinnia mesophyll cells in culture." Plant Cell Physiol.30. 243-252 (1989)

[Publications] Fukuda,H.,Kobayashi,H.: "Dynamic organization of the cytoskeleton during tracheary-element differentiation." Develop.Growth Differ.31. 9-16 (1989)

[Publications] 福田裕穂: "細胞分化と細胞骨格" 細胞. 21. 306-310 (1989)

[Publications] 西村いくこ: "オルガネラの形成ー単膜系オルガネラ、“現代植物生理学第3巻 成長と分化"柴岡弘郎編" 朝倉書店, (1990)

[Publications] Akira Watanabe: "Cell Separation in Plants" Springer-Verlag, 31-38 (1989)

[Publications] 鎌田博: "植物バイオテクノロジー事典" 日本エンタープライズ社(東京), 235 (1989)

渡辺昭名古屋大学, 農学部, 教授 (70023471)

[Publications] 西村いくこ: "オルガネラの形成ー単膜系オルガネラ、“現代植物生理学第3巻成長と分化"柴岡弘郎編" 朝倉書店, (1990)