• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1991 Fiscal Year Final Research Report Summary

System for fluorometry of carboxylic acids under mild condition

Research Project

Project/Area Number 01860014
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

OHRUI Hiroshi  Tohoku Univ., Agric., Assistant Prof, 農学部, 助教授 (20100050)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hiroaki  Tosoh, Chief Researcher, 主任研究員
AKASAKA Kazuaki  Tohoku Univ., Agric., Associate Prof., 農学部, 助手 (10201881)
NISHIDA Yoshihiro  Tohoku Univ., Agric., Associate Prof., 農学部, 助手 (80183896)
Project Period (FY) 1989 – 1991
Keywordshydroxy fatty acid / hydroperoxy fatty acid / 9-bromomethylacridine / column switching
Research Abstract

9-Acridinylmethyl esters of fatty acids gave molecular ions with relatively strong intensity and simple fragmentation patterns. In the spectra of unsaturated fatty acid esters, the 26 mass unit separations were observed at the position of double bonds in the molecule. Ions (M-30)^+ and (M-31)^+ suggested the existence of a hydroxymethyl group and the 29 and 30 mass unit separations suggested the positions of hydroxy groups located in the molecule. These fragmentations were useful for the structural analysis of fatty acids.
Hydroxy and hydroperoxy fatty acids were labeled with 9-bromomethylacridine at room temperature. They were separated from the degradation produce and less polar fatty acid derivatives on an octyl silicagel column, and were loaded into an octadecyl silicagel column by the on-line column switching. By the method, picomolar levels of the derivatives were determined with good reproducibility. The detection limit of 16-hydroxyhexadecanoic acid as a representative was 0.9 pmol (S/N=3) and the relative standard deviation of its peak areas was 2.5% (18.5 pmpl,n=7). The method was applied to the determination of the hydroxy fatty acids derived from phosphatidylcholine (PC) hydroperoxides spiked in human plasma. During the incubation at 37゚C for 4h, 2.5-3% of PC hydroperoxides decreased gave the corresponding hydroxy fatty acids.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Kazuaki Akasaka: "Structural Analysis of 9-Acridinylmethyl Derivatives of Fatty Acids by Mass Spectrometry" Analytical sciences. 6. 511-514 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Akasaka: "Determination of Hydroxy and Hydroperoxy Fatty Acids by a High Performance Liquid Chromatography with a column Switching System" Biosci.Biotech.Biochem.submited for publication.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Akasaka: ""Structural Analysis of 9-Acridinylmethyl Derivatives of Fatty Acids by Mass Spectrometry"" Analytical Sciences. Vol. 6. 511-514 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuaki Akasaka: "Determination of Hydroxy and Hydroperoxy Fatty Acids by a High Performance Liquid Chromatography with a column Switching System"" Biosci. Biotech. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1994-03-18  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi