1989 Fiscal Year Annual Research Report

呼吸中枢作用薬の定量的薬効評価用汎用統合型ソフトウェアの開発

Research Project

Project/Area Number	01870012
Research Category	Grant-in-Aid for Developmental Scientific Research
Research Institution	Jikei University School of Medicine
Principal Investigator	福原武彦東京慈恵会医科大学, 医学部, 教授 (10009889)
Co-Investigator(Kenkyū-buntansha)	西牟田啓日本電気三栄株式会社, 医用電子機器事業部第3部, 部長加藤総夫東京慈恵会医科大学, 医学部, 助手 (20169519)
Keywords	呼吸中枢 / 神経薬理学 / 横隔神経 / 時系列解析 / 生体信号情報処理 / 呼吸調節 / 薬効評価 / 神経活動電位
Research Abstract	平成元年度においては、1.ソフトウェア開発、2.動物実験およびデ-タ解析を平行して進めた。 1.ソフトウェア開発:シグナル・プロセッサ-7T17(日本電気三栄、現有設備)を用いて、信号処理専用高級言語Signal BASICーversion5(日本電気三栄)により、呼吸性活動解析プログラムパッケ-ジ「RESPACK」(仮称)の第1版を作製した。本プログラムは以下の7サブモジュ-ルからなる:(1)呼吸性活動のディジタル信号化モジュ-ル、(2)呼吸性活動抽出モジュ-ル、(3)呼吸周期、吸息ならびに呼息持続時間およびその変動係数の自動計測モジュ-ル、(4)呼吸性活動の自己ならびに相互パワ-スペクトル密度関数推定モジュ-ル、(5)解析結果のディスプレイ表示、およびプリンタ、プロッタ出力モジュ-ル、(6)解析結果のフロッピィディスク入出力モジュ-ル、および、(7)解析結果の外部端末(パ-ソナル・コンピュ-タ等)出力モジュ-ル。 2.動物実験と解析デ-タ:麻酔下に非動化した人工呼吸下のウサギから、横隔神経、迷走ならびに舌下神経の遠心性発射活動を双極白金電極を用いて同時に導出し、デ-タレコ-ダを用いて、磁気テ-プに収録した。上述の標本に大腿静脈より、pentobarbital、morphine、neloxoneならびにdoxapramを適用し、その急性作用を経時的に観察し、磁気テ-プに記録した。上述の「RESPACK」を用いてデ-タを解析した結果、呼吸中枢の活動状態を的確に表現する指標が正確かつ簡便に得られることが示され、これらの薬物の作用について以下の新知見を得た:(1)呼吸リズムとその安定性の維持機構に対する薬物の作用態度ならびに機序が異なる可能性(上記(3)のモジュ-ルによる解析結果に基づく)ならびに、(2)呼吸リズムと運動神経の吸息性活動の形成および修飾に関与する呼吸中枢内神経回路群の薬物感受性が異なる可能性(上記(4)のモジュ-ルによる解析結果に基づく)。

Research Products

(17 results)

All Other

All Publications (17 results)

[Publications] Hukuhara,Takehiko: "Quantification of the high-frequency oscillation in the inspiratory nerve activities by power spectral density estimation and non-linear least squares method." Japanese Journal of Pharmacology. 52(Suppl.). 323 (1990)
[Publications] Hukuhara,Takehiko: "Respiratory phase-related fluctuation of the high-frequencyoscillation in the phrenic nerve activity." Proc.of the XXXI International Unionof Physiological Sciences. 71 (1989)
[Publications] Hukuhara,Takehiko: "Interrelation and superposition of respiratory and cardivascular rtythms in brainstem reticular unit activity,the sympathetic nerve activity and EEG.Quantitative spectral analysisin cats and rabbits." Proc.of XXXI International Congress of Physiologocal Science:Satellite Symposium on Cardiorespiratory and Motor Coordination(Springer-Verlag.Berlin). (1990)
[Publications] Hukuhara,Takehiko: "Organization of the brain stem neural mechanisms for generation of respiratory rhythm." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)
[Publications] Hukuhara,Takehiko: "Effects of vagal afferent stimulation on high-frequency ocillation in phrenic nerve activity of rabbits." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)
[Publications] 福原武彦: "中枢性調節神経機構における連関" 自律神経. 26(4). 348-353 (1989)
[Publications] 福原武彦: "呼吸調節と自律神経" Clinical Neuroscience. 7(7). 17-19 (1989)
[Publications] 福原武彦: "自律神経と呼吸機能" 臨床成人病. 19(8). 1329-1334 (1989)
[Publications] Kato,Fusao: "Effect of morphine on bilateral synchronization of high-frequency oscillation in phrenic nerve activity." Japanise Journal of Pharmacology. 49. 166 (1989)
[Publications] Kato,Fusao: "Regional difference in respiratory-phase related fluctuation of high-frequency oscillations in the phrenic and cranial nerve activities in rabbits." Japanese Journal of Physiology. 39Suppl.250 (1989)
[Publications] Kato,Fusao: "Different course in the phase-related fluctuation of the high-frequency oscillation in the phrenic and cranial nerve activities in rabbits." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)
[Publications] 加藤総夫: "両側横隔神経および迷走神経遠心性活動の関連度と高頻度同期波の同期過程" 呼吸(Respiration Research). 8(4). 456 (1989)
[Publications] Takeda,Ryuji: "Diazepam potentiates inhibition in bulbar respiratory neurons of cats." Respiration Physiology. 77(2). 173-186 (1989)
[Publications] Takeda,Ryuji: "Diazepam potentiates postsynaptic inhibition in bulbar respiratory neurons of cats." Respiration Physiology. 77(2). 173-186 (1989)
[Publications] Takeda,Ryuji: "Respiratory pattern generation in the ventral respirtory group neurons." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)
[Publications] Sakai,Yuji: "Stereotyped phrenic responses to mechanical stimulation of the epiglottal mucosa." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)
[Publications] 高野一夫: "横隔神経高頻度同期波と脳幹部呼吸性ニュ-ロン活動の相関" 自律神経. 27(1). 29-34 (1990)

1989 Fiscal Year Annual Research Report

呼吸中枢作用薬の定量的薬効評価用汎用統合型ソフトウェアの開発

Principal Investigator

福原 武彦 東京慈恵会医科大学, 医学部, 教授 (10009889)

Research Products

[Publications] Hukuhara,Takehiko: "Quantification of the high-frequency oscillation in the inspiratory nerve activities by power spectral density estimation and non-linear least squares method." Japanese Journal of Pharmacology. 52(Suppl.). 323 (1990)

[Publications] Hukuhara,Takehiko: "Respiratory phase-related fluctuation of the high-frequencyoscillation in the phrenic nerve activity." Proc.of the XXXI International Unionof Physiological Sciences. 71 (1989)

[Publications] Hukuhara,Takehiko: "Organization of the brain stem neural mechanisms for generation of respiratory rhythm." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)

[Publications] Hukuhara,Takehiko: "Effects of vagal afferent stimulation on high-frequency ocillation in phrenic nerve activity of rabbits." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)

[Publications] 福原武彦: "中枢性調節神経機構における連関" 自律神経. 26(4). 348-353 (1989)

[Publications] 福原武彦: "呼吸調節と自律神経" Clinical Neuroscience. 7(7). 17-19 (1989)

[Publications] 福原武彦: "自律神経と呼吸機能" 臨床成人病. 19(8). 1329-1334 (1989)

[Publications] Kato,Fusao: "Effect of morphine on bilateral synchronization of high-frequency oscillation in phrenic nerve activity." Japanise Journal of Pharmacology. 49. 166 (1989)

[Publications] Kato,Fusao: "Regional difference in respiratory-phase related fluctuation of high-frequency oscillations in the phrenic and cranial nerve activities in rabbits." Japanese Journal of Physiology. 39Suppl.250 (1989)

[Publications] Kato,Fusao: "Different course in the phase-related fluctuation of the high-frequency oscillation in the phrenic and cranial nerve activities in rabbits." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)

[Publications] 加藤総夫: "両側横隔神経および迷走神経遠心性活動の関連度と高頻度同期波の同期過程" 呼吸(Respiration Research). 8(4). 456 (1989)

[Publications] Takeda,Ryuji: "Diazepam potentiates inhibition in bulbar respiratory neurons of cats." Respiration Physiology. 77(2). 173-186 (1989)

[Publications] Takeda,Ryuji: "Diazepam potentiates postsynaptic inhibition in bulbar respiratory neurons of cats." Respiration Physiology. 77(2). 173-186 (1989)

[Publications] Takeda,Ryuji: "Respiratory pattern generation in the ventral respirtory group neurons." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)

[Publications] Sakai,Yuji: "Stereotyped phrenic responses to mechanical stimulation of the epiglottal mucosa." Control of Breathing and Dyspnea(Advances in the Bioscience Ser.)〈T.Takishima&N.S.Cherniack.eds〉,Pergamon Press,Oxford. (1990)

[Publications] 高野一夫: "横隔神経高頻度同期波と脳幹部呼吸性ニュ-ロン活動の相関" 自律神経. 27(1). 29-34 (1990)

福原武彦東京慈恵会医科大学, 医学部, 教授 (10009889)