Development of a pulsatile perfusion apparatus for heart preservation with myocardial impedance monitor

1991 Fiscal Year Final Research Report Summary

• Project/Area Number: 01870059
• Research Category: Grant-in-Aid for Developmental Scientific Research
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Osaka University
• Principal Investigator: KANEKO Mitsunori Osaka Univ Med School, assist prof, 医学部, 助手 (70169580)
• Co-Investigator(Kenkyū-buntansha): FUKUSHIMA Noriatsu Osaka Univ Med School, Senior Resid, 医学部附属病院, 医員 ; MIYAMOTO Yuji Osaka Univ Med School, assist Prof, 医学部, 助手 (80229898) ; KADOBA Keishi Osaka Univ Med School, assist Prof, 医学部, 助手 (00185886) ; NAKATA Seizoh Osaka Univ Med School, assist Prof, 医学部, 助手 (50116068) ; SHIRAKURA Ryota Osaka Univ Med School, assoc Prof, 医学部, 助教授 (00116047)
• Project Period (FY): 1989 – 1990
• Keywords: heart preservation / pulsatile flow / heart transplantation / impedance

Research Abstract

This study was done to develop a portable perfusion apparatus for heart preservation in which hearts are hypothermically perfused with pulsatile pump for 24 hours and myocardial impedance is continuously measured to assess the myocardial edema. At first we evaluated simple immersion methods for preservation as control with Langendorf using isolated rabbit heart. The left ventricular function after 24 preservation in UW solution was better than in saline and modification at reperfusion, leukocyte depleted terminal cardioplegia, enhanced LV function to sustain the normal circulation after orthotopic heart transplantation in dog. The portable perfusion apparatus with a diaphragm driven by electromagnetic power which derivesd pulsatile flow was made. In vitro test with the apparatus showed 0.05-5ml of stroke volume, 60-120 beat/min, and 15-150 mmHg of perfusion pressure which could be controllable for isolated rabbit heart perfusion. The temperature, however, was difficult to maintain to 10 C during 24 hours perfusion because of the effect of room temperature especially when a "portable" apparatus was desired. Moreover conventional impedance meter should be used at constant temperature and it was proved that the value of tissue impedance at variable temperature was unreliable and worthless.

Research Products

• [Publications] Fukushima N,Nakano S,Shirakura R.: "Effects of terminal cordioplegia with leukocyte-depleted blood on myocardial ATP of heart preserved 24 hours." Transplantation Proceedings.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shirakura R,Matsuda H,Nakano S,Kaneko M,Kawaguchi A,Miyamoto Y,Matsuwaka R,Kitagawa S,Fukushima N,Kawashima Y.: "24-hours storage for asphyxiated canine hearts with use of Belzer University of Wisconsin solution." Transplantation Proceedings. 23. 662-663 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shirakura R,Matsuda H,Nakano S.: "A new method to harvest multiple organs from on-heart-beating donor byuse of percutaneous cardiopulmonary supporting machiene." Transplantation Proceedings.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shirakura R,Matsuda H,Nakano S,Nakata S,Kaneko M,Miyamoto Y,Matsuwaka R,Kitagawa S,Fukushima N,Kawashima Y.: "Cavdiac function and myocardial performance of 24hour preserved asphyxiated canine heart." Ann Thorac Surg. 53. 440-444 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fukushima N,Nakano S,Shirakura R,Matsuda H.: "Effects of terminal cardioplegia with leuko-cyte depleted blood on heart graft preserved 24hours"
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fukushima N, Nakano S, Shirakura R.: "Effects of terminal cardioplegia with leukocyte-depleted blood on myocardial ATP of heart preserved 24 hours." Transplantation Proceedings.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shirakura R, Matsuda H, Nakano S, Nakata S, Kaneko M, Kawaguchi A, Miyamoto Y, Matsuwaka R, Kitagawa S, Fukushima N, Kawashima Y.: "24-hours storage for asphyxiated canine hearts with use of Belzer University of Wisconsin solution." Transplantation Proceedings.23. 662-3 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shirakura R, Matsuda H, Nakano S.: "A new method to harvest multiple organs from non-heart-beating donor by use of percutaneous cardiopulmonary supporting machine." Transplantation Proceedings.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shirakura R, Matsuda H, Nakano S, Nakata S, Kaneko M, Miyamoto Y, Matsuwaka R, Kitagawa S, Hukushima N, Kawashima Y.: "cardiac function and myocardial performance of 24 hour preserved asphyxiated canine heart." Ann Thorac Surg.53. 440-4 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fukushima N, Nakano S, Shirakura R.: "Effects of terminal cardioplegia with leukocyte-depleted blood on heart graft preserved 24 hours."
  • Description: 「研究成果報告書概要(欧文)」より