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1991 Fiscal Year Final Research Report Summary

Development of a new cytotoxicity test method for dental materials

Research Project

Project/Area Number 01870080
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 補綴理工系歯学
Research InstitutionTOKYO MEDICAL and DENTAL UNIVERSITY

Principal Investigator

SATO Atsushige  Tokyo Medical and Dental University, Faculty of Dentistry, Department of Biomaterials Science, Professor, 歯学部, 教授 (40045985)

Co-Investigator(Kenkyū-buntansha) HONGO Toshio  Tokyo Medical and Dental University, Faculty of Dentistry, Department of Biomate, 歯学部, 助手 (60142444)
SATO Kazuko  Tokyo Medical and Dental University, Faculty of Dentistry, Department of Biomate, 歯学部, 助手 (50046083)
Project Period (FY) 1989 – 1991
KeywordsCytotoxicity test method / SV-40 large T antigen / Human dental pulp / Mouse calvaria / Odontoblast / Osteoblast / Intracellular Ca^<2+> / Neutral red assay
Research Abstract

In order to develop a new cytotoxicity test method for assessing possible toxic effects of dental materials, the study was performed to establish new lines of cultured cells with highly susceptible to toxicants and quantitative indexes for cytotoxicity tests.
1. The human dental pulp cells and mouse calvaria cells were transfected with the SV-40 large T antigen. Four immortalized cell lines, LSC cells from human pulp and DMCs LMCs and VMCs cells from mouse calvaria were obtained. These cell lines preserved some of the properties of the dental pulp or the osteoblast.
2. The susceptibility of four cytotoxicity assays were compared. The MTT and NR assays showed higher susceptibility compared to the agar overlay assay and millipore filter method. The MTT assay showed the highest reproducibility among four tests.
3. The intracellular Ca ion concentration ([Ca^<2+>]i) of JTC-12 cells was measured employing Fura 2 AM fluorescence. Exposing cells to low concentrations of HgCl_2, which had no apparent effect on viability, produced a rapid and prolonged increase in [Ca^<2+>]i. The [Ca^<2+>]i was a useful index to detect the initial lesion of cultured cells exposed to dental materials.
4. These results suggest that the new cell lines and the [Ca^<2+>]i change may be useful in the future research for testing the toxicity of the dental materials.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] RIKITAKE,Y.: "A newly established cell line derived from the human dental pulp." Proc.Japan Acad.65B(8). 187-190 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 力武 康次: "ヒト歯髄由来美容細胞のプラスミドpMT1-neo遺伝子導入による不死化について" 口病誌. 56(4). 540-560 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森末 裕行: "細胞培養法を応用した重金属の臓器毒性に関する基礎的研究" 口病誌. 56(2). 244-262 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATO,A.: "Mutagenicity of dental nickel-chromium alloys." Dentistry in Japan. 28. 129-135 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤 温重: "バイオマテリアルのin vitro毒性試験の実情" J.Toxicol.Sci.15(3). 179 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤 温重: "歯科材料の簡易・高感受細胞毒性試験法の開発" 歯科材料・器械. 9(16). 110 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 五十嵐 良明: "細胞毒性試験の標準化-標準試料の作成とそれを用いた各種試験法の比較" 第12回日本バイオマテリアル学会大学 予稿集. 61 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATO,A.: "Studies on the standardization of cytotoxicity testing for dental materials." Tissue Culture in Dent.27. 33-35 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATO,A.: "Intracellular[Ca^<2+>]and cellular injury by nephrotoxic chemicals in renal cell cultures." Japan.J.Pharm.55Suppl.47P (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SATO,A.: "Toxicity tests of biomaterials in japan." In Vitro. 27. 172A (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] RIKITAKE,Y.: "A Newly established cell line derived from the human dental pulp" Proc.Japan Acad.65B(8). 187 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] RIKITAKE,Y.: "Immortalization of human dental pulp cells with transfecting of the plasmid pMT1-neo" J.Stomatol.Soc.Jpn.56(2). 540 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MORISUE,H.: "Application of renal epithelial cell culture in study of organ toxicity of heavy metals" J.Stomatol.Soc.Jpn.56(2). 244 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATO,A.: "Mutagenicity of dental nickel-chromium alloys" Dent. in Japan. 28. 129 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATO,A.: "In vitro toxicity tests of biomaterials" J.Toxicol.Sci.15(3). 179 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATO,A.: "A new in vitro cytotoxicity test method for dental materials" J.J.Dent.Mat.9(Suppl.). 110 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] IKARASHI,Y.: "Standardization for cytotoxicity test:Preparation of reference standard materials and comparison of the results using various test methodologies." Proc.the 12th Ann. Meet.Jap.Soc. for Biomater.61. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATO,A.: "Studies on the standardization of cytotoxicity testing for dental materials." Tissue Culture in Dent.27. 33 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATO,A.: "Intracellular[Ca^<2+>] and cellular injury by nephrotoxic chemicals in renal cell cultures." Japan.J.Pharm.55(Suppl.1). 47 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SATO,A.: "Toxicity tests of biomaterials in Japan." In vitro. 27. 172A (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-18  

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