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1991 Fiscal Year Final Research Report Summary

Reseach on development of a drug from an endogeneous peptide that inhibits the platelet aggregation.

Research Project

Project/Area Number 01870099
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionOkayama University

Principal Investigator

OHMORI Shinji  Okayama University, Professor, 薬学部, 教授 (10032872)

Co-Investigator(Kenkyū-buntansha) SAKAUE Takahiro  Senju Pharmaceutical Co. Ltd. Res. Assoc., 研究所, 所員
OGATA Kazumi  Senju Pharmaceutical Co. Ltd. Res. Director, 研究所, 部長
TSUBOI Seiji  Okayama University, Res. Assoc. Faculty of Pharmaceutical Sciences, 薬学部, 助手 (50172052)
IKEDA Mikiko  Okayama University, Assistant prof., 薬学部, 助教授 (20112154)
Project Period (FY) 1989 – 1991
KeywordsPlatelet aggregation / Inhibition / Glutathione / Synthesizing - enzyme / S - Carboxyethyl / DNA synthesis / Rat hepatocyte / Regenerating rat liver
Research Abstract

1. A high-performance liquid chromatographic determination of S-(2caroxypropyl)glutathione in vegetable was established. By this method the peptide was determined in various vegetables.
2. S-(1, 2-Dicarboxyethyl)glutathione(DCE-GS)exerted a potent inhibition effect on ADP- or thrombin-induced platelet aggregation. The peptide, however, did not inhibit the platelet aggregation induced by 12-o-tetradecanoylphorbol-13-acetate.
3. The DCE-GS-synthesizing enzyme was, purified from rat liver cytosolic fraction. The molecular mass of the enzyme was 53 kDa. The Km values for GSH and L-malate were 2.3 and 4.0 mM.
4. DCE-GS alone had no effect on DNA synthesis of hepatocytes. However, when it was used with, - EGF, it effectively enhanced EGF-st'imulated DNA synthesis of hepatocytes.
5. The DCE-GS level increased in regenerating rat liver, reaching a maximum level on the 2nd day and reverted to normal level in a week.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Seiji Tsuboi: "Increase of S-(1,2-dicarboxyethyl)glutathione in regenrating rat liver" Res.Exp.Med.(1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiji Tsuboi: "Inhibitory effects of S-(1,2-dicarboxyethyl)glutathione on collagen-induced platelet aggregation" J.Biochem.(1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiro Miyazaki: "Enhancing effect of S-(1,2-dicarboxyethyl)glutathione on epidermal growth factor-stimulated DNA synthesis in primary cultures of adult rat hepatocytes" Res.Exp.Med.190. 381-387 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiji Tsuboi: "Inhibition of platelet aggregation by S-(1,2-dicarboxyethyl)glutathione,intrisic tripeptide in liver,heart and lens" Arch.Biochem.Biophys.279. 146-150 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiji Tsuboi: "S-(1,2-Dicarboxyethyl)glutathione and activity for its synthesis in rat tissues" J.Biochem.107. 539-545 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiji Tsuboi: "S-(2-Carboxypropyl)glutathione in vegetables of liliflorae" J.Agric.Food Chem.37. 611-615 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seiji Tsuboi: "Increase of S-(1, 2-dicarboxyethyl)glutathione in regenerating rat liver" Res. Exp. Med.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seiji Tsuboi: "Inhibitory effects of S-(1, 2-dicarboxytethyl)-glutathione on collagen-induced platelet aggregation" J. Biochem.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiro Miyazaki: "Enhancing effect of S-(1, 2-dicarboxyethyl)-glutathione on epidermal growth factor-stimulated DNA synthesis in primary cultures of adult rat hepatocytes" Res. Exp. Med.190. 381-387 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seiji Tsuboi: "Inhibition of platelet aggregation by S-(1, 2-dicarboxyethyl)glutathione, intrisic tripeptide in liver, heart and lens" Arch. Biochem. Biophys.279. 146-150 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seiji Tsuboi: "S-(1, 2-Dicarboxyethyl)glutathione and activity for its synthesis in rat tissues" J. Biochem.107. 539-545 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seiji Tsuboi: "S-(2-Carboxypropyl)glutathione in vegetables of liliflorae" J. Agric. Food Chem.37. 611-615 (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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