1991 Fiscal Year Final Research Report Summary
Reseach on development of a drug from an endogeneous peptide that inhibits the platelet aggregation.
| Project/Area Number |
01870099
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Okayama University |
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Principal Investigator |
OHMORI Shinji Okayama University, Professor, 薬学部, 教授 (10032872)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAUE Takahiro Senju Pharmaceutical Co. Ltd. Res. Assoc., 研究所, 所員
OGATA Kazumi Senju Pharmaceutical Co. Ltd. Res. Director, 研究所, 部長
TSUBOI Seiji Okayama University, Res. Assoc. Faculty of Pharmaceutical Sciences, 薬学部, 助手 (50172052)
IKEDA Mikiko Okayama University, Assistant prof., 薬学部, 助教授 (20112154)
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| Project Period (FY) |
1989 – 1991
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| Keywords | Platelet aggregation / Inhibition / Glutathione / Synthesizing - enzyme / S - Carboxyethyl / DNA synthesis / Rat hepatocyte / Regenerating rat liver |
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| Research Abstract |
1. A high-performance liquid chromatographic determination of S-(2caroxypropyl)glutathione in vegetable was established. By this method the peptide was determined in various vegetables.
2. S-(1, 2-Dicarboxyethyl)glutathione(DCE-GS)exerted a potent inhibition effect on ADP- or thrombin-induced platelet aggregation. The peptide, however, did not inhibit the platelet aggregation induced by 12-o-tetradecanoylphorbol-13-acetate.
3. The DCE-GS-synthesizing enzyme was, purified from rat liver cytosolic fraction. The molecular mass of the enzyme was 53 kDa. The Km values for GSH and L-malate were 2.3 and 4.0 mM.
4. DCE-GS alone had no effect on DNA synthesis of hepatocytes. However, when it was used with, - EGF, it effectively enhanced EGF-st'imulated DNA synthesis of hepatocytes.
5. The DCE-GS level increased in regenerating rat liver, reaching a maximum level on the 2nd day and reverted to normal level in a week.
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