1991 Fiscal Year Final Research Report Summary
Animal Experimental Model of Human Hepatocellular Carcinoma
| Project/Area Number |
01880010
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
SHIGA Junji University of Tokyo Pathology Associate Professor, 医学部(病)病理, 助教授 (10110694)
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| Co-Investigator(Kenkyū-buntansha) |
HACHISU Tatsuyuki Center of Shibayagi Chief Room Investigator, 研究室長
HARASAWA Ryou University of Tokyo Animal Fascility Associate Professor, 医学部・動物実験施設, 助教授 (70159101)
ABE Kenji National Institute of Health Pathology Chief Investigator, 病理, 主任研究官 (60130415)
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| Project Period (FY) |
1989 – 1991
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| Keywords | Woodchuck / Woodchuck hepatitis virus / Hepatic cancer / PCR / RIA |
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| Research Abstract |
Basical problems about conditions of care and breeding woodchucks such as cages, housing or food were solved. We made stainless steel indoor cages(O. 36cm^2 floor space) which are very useful for keeping animals to observe them or make experiment but in this cage they did not mate or breed. In larger indoor iron boxes(2 m^2) animals could breed but new born pups were often killed by their dams. In our conclusion the best circumstances for breeding is outdoor permanent above ground cages in which litter size same as those of wild animals were obtained. Food pellets we produced are same as those of guinea pigs containing less crude fibers.
We succeeded in establishing a method for obtaining artificial virus carriers by injecting virus into non virus carrier pupps. Even in 6 weeks old pupps we could get positive results. We conclude 2-4 weeks old pupps are best to be injected with virus as they are very active with open eyes and mortality is already low. Newly obtained carriers do not still develop cancers. We have checked positivity of hepatitis virus by RIA for surface antigen but we developed new technique utilizing polymerase chain reaction. As primer we used DNA part coding surface antigen.
We followed wild spontaneous carriers for detecting hepatic cnacers and found when volume of cancer become larger than 10cm^3 they develop very rapidly. Integration pattern of virus DNA and n-myc rearrangement suggested that cancer develop both multicentric and metastatic. C-myc rearrangement occur very rare.
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