1990 Fiscal Year Final Research Report Summary
Alternative to Breast Carcinogenicity Test Using a Tissue Culture Method
| Project/Area Number |
01880013
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
SUZUKI Kunio Inst. Phys. Chem. Res., Anim. Cell. Lab. Senior Research Scientist, 動物細胞システム研究室, 副主任研究員 (60100054)
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| Co-Investigator(Kenkyū-buntansha) |
MITSUOKA Tomotari Inst. Phys. Chem. Res., Frontier Res. Prog., Head, 国際フロンティア研究システム, チームリーダー (30157549)
MIZUTANI Takeo Inst. Phys. Chem. Res., Lab. Anim. Ctr. Senior Technical Scientist, 動物試験室, 室長 (30087598)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Breast cancer / Carcinogens / Mammary epitherial cell / Micronucleus / Alternative |
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| Research Abstract |
We developed a new in vitro micronucleus test using cultured mouse mammary epithelial cells as a tisure-specific screen for suspected breast carcinogens.
Mammary epithelial cells from pregnant mice were cultured in serum free Ham's F-12/Dulbecco's modified Eagle's medium supplemented with insulin, epidermal growth factor, transfering, cholera toxin, bovine serum albumin and antibiotics. Several breast carcinogens and other chemicals were added to the cultures at day 6 of culture, and the number of micronuclei per 1,000 cells was scored at 24hr after treatment.
In the present study, five breast carcinogens, N-methyl-N-nitrosourea, N-butyl-N-nitrosorea, 7, 12-dimethylbenz (a) anthracene, 20-methylcholanthrene and 2-acetamidofluorene, and one direct carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine increased the micronuclei incidence in the cultures.
Since all breast carcinogens and a direct carcinogen induced micronuclei in the culture of mouse mammary epitherial cells in a dose related manner, but other agents generally do not, these results justify the use of micronuclei as a short term, in vitro screen of suspected breast carcinogens.
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