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1990 Fiscal Year Final Research Report Summary

Alternative to Breast Carcinogenicity Test Using a Tissue Culture Method

Research Project

Project/Area Number 01880013
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

SUZUKI Kunio  Inst. Phys. Chem. Res., Anim. Cell. Lab. Senior Research Scientist, 動物細胞システム研究室, 副主任研究員 (60100054)

Co-Investigator(Kenkyū-buntansha) MITSUOKA Tomotari  Inst. Phys. Chem. Res., Frontier Res. Prog., Head, 国際フロンティア研究システム, チームリーダー (30157549)
MIZUTANI Takeo  Inst. Phys. Chem. Res., Lab. Anim. Ctr. Senior Technical Scientist, 動物試験室, 室長 (30087598)
Project Period (FY) 1989 – 1990
KeywordsBreast cancer / Carcinogens / Mammary epitherial cell / Micronucleus / Alternative
Research Abstract

We developed a new in vitro micronucleus test using cultured mouse mammary epithelial cells as a tisure-specific screen for suspected breast carcinogens.
Mammary epithelial cells from pregnant mice were cultured in serum free Ham's F-12/Dulbecco's modified Eagle's medium supplemented with insulin, epidermal growth factor, transfering, cholera toxin, bovine serum albumin and antibiotics. Several breast carcinogens and other chemicals were added to the cultures at day 6 of culture, and the number of micronuclei per 1,000 cells was scored at 24hr after treatment.
In the present study, five breast carcinogens, N-methyl-N-nitrosourea, N-butyl-N-nitrosorea, 7, 12-dimethylbenz (a) anthracene, 20-methylcholanthrene and 2-acetamidofluorene, and one direct carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine increased the micronuclei incidence in the cultures.
Since all breast carcinogens and a direct carcinogen induced micronuclei in the culture of mouse mammary epitherial cells in a dose related manner, but other agents generally do not, these results justify the use of micronuclei as a short term, in vitro screen of suspected breast carcinogens.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells grown under serumーfree culture conditions" Enviromental and Molecular Mutagenesis. 14. 195 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木 邦夫: "動物の組織および細胞に特異的な発癌物質検出法" バイオインダストリ-. 7. 49-55 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Research. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunio Suzuki: "In Vitro micronuclei in the mouse mammary epitherial cells ground under serum-free culture conditions" Environm. Mol. Mutagen. 14. 195 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunio Suzuki: "Animal tissue and cell specific screening test for carcinogens (in Japanese)" Bio Industry. 7. 49-55 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Res.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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