1991 Fiscal Year Final Research Report Summary
Rapid Determination of Phytate and Other Inositol Phosphates
| Project/Area Number |
01880018
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
家政学
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| Research Institution | Tachikawa College of Tokyo |
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Principal Investigator |
YOSHIDA Tsutomu Tachikawa College of Tokyo, Department of Food and Nutrition, Professor, 食物学科, 教授 (90073543)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAWA Eiji Seijo Junior College, Department of Human and Environment, Professor, 教養科, 教授 (10119365)
SHINODA Shoko Tachikawa College of Tokyo, Department of Food and Nutrition, Assistant, 食物学科, 助手 (40132055)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Phytate / Ion exchange chromatography / Inositol phosphates / Mineral |
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| Research Abstract |
Sodium phytate added into the diets usually reduce the availability of minerals such as Ca, Mg, Fe, Zn in the gastrointestinal tract of rats. However almost 60% of phytate is hydrolysed in the small intestine and the cecum of rats. Hydrolysis of phytate(IP_6)was usually discussed about amount of phytate left in the rat feces. Effects of inositol penta-(IP_5), tetra(IP_4), tri-(IP_3), di-(IP_2), mono-(IP_1)phosphates on mineral availability in the gastrointestinal tracts have not been discussed, because there is no rapid determination of these inositol phosphatase. The objective of this research was to establish a direct injection method, for phytate and other inositol phosphates determination. An indirect photometric ion chromatographic method using a HPLC system with UV detector(matsunaga, et. al., 1988)was carried out for the determination of phytate and other inositol phosphates. Sodium phytate was boiled in 6N-HCl for 2 hours then evaporated to dryness after washing with H 0. Precipitate was disolved in H 0 and was applied to an anion exchange HPLC system. The inositol hexa-phosphate could be separated from other inositol phosphtes(IP_5 -IP_1). Ther were peaks which assumed to be IP_5, and IP_4-IP_1(sometimes IP_5, IP_4, IP_3-IP_1). They were not identified because no standards were available. Momen and Kinugoshi tofe with different kinds of coagurant were analyzed for IP_6 by using this method. Inositol hexaphosphate in samples of Momen tofu was able to separated and has good analytical values, but not in samples of Kinugoshi tofu.
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