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1991 Fiscal Year Final Research Report Summary

Embryological studies on the genesis of ano-rectal malformations, using pig models.

Research Project

Project/Area Number 02044024
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionInstitute of Clinical Medicine

Principal Investigator

OHAKWA Haruo  Professor, Institute of Clinical Medicine, Tsukuba University, 臨床医学系, 教授 (30009667)

Co-Investigator(Kenkyū-buntansha) HORI Tetsuo  Associate PROF. INST. of Clinical MED., UNIV. of Tsukuba, 臨床医学系, 講師 (80173615)
PUTTE Scfoon  ユトレヒト大学, 医学部(オランダ王国), 助教授
MATSUMOTO Masatomo  Assistant, INST. of Clinical MED., Tsukuba University, 臨床医学系, 助手 (00229569)
VAN DER PUTTE SCJ  Associate Professor, DEPT. of MED., Utrecht University
Project Period (FY) 1990 – 1991
KeywordsAno-rectal malformations / Anal atresia / Pig model with anal atresia / Cloaca / Cloacal plate / Internal anal sphincter / Three dimensional reconstruction / 三次元立体構築
Research Abstract

An ano-rectal malformation is one of the most important diseases in pediatric surgery. Various operative methods and their variations have been devised so far. But the operative results of high type of anomalies have not been necessarily satisfactory at all. We have tried to solve these problems from an embryological point of view.
We have already established 2 families of pig model of ano-rectal malformations and have been capable to get anomalous pigs and foetuses from experimental matings. Pig foetuses of 25-60 days were obtained systematically, but there are still defective gestational ages of foetuses. They were supplemented by the materials of University of Utrecht by this co-opearative study, which had been produced from their already extinguished family.
The results of the present studies are followed.
1) Two pig families with malformations have been maintained by Tsukuba University team.
2) Abnormal foetuses of 22-60 days were successfully obtained. But younger ages than 22 days w … More ere not obtained, and they were concluded to be impossible to get in the present method of abortion.
3) Early embryological developments of anorectal malformations have been studied with these serially sectioned foetuses. They were studied in combination with those materials in the cooperative research with Utrecht University.
4) Especially for the three dimensional reconstruction studies, over 1, 000 serial sections were necessary from each foetus.
5) The progressive process of the partition between the ano-rectum and urogenital sinus from original cloacal system were ascertained. It was shown by the three dimensional pictures from our own devices. (Mebio 9(1), 1992)
6) Classical explanation and concept of "cloacal membrane" was denied by our research. The defect of cloacal plate between the dorsal cloaca and tail groove were suggested to be a cause of anal atresia. This unsuccessful partition around 30 days in the case of pig is a cause of the anomaly.
7) The inductive role of the cloacal plate was confirmed. But the true story must be confirmed by the materials before 21 days in the future.
8) The development of internal sphincter was confirmed in normal abnormal pigs.
9) The sex difference in the development of anal atresia were clearly explained by this research embryologically. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] H.OHKAWA,M.MATSUMOTO: "On the sex difference in the genesis of anorectal malformations." J.Pediatric Surgery. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松本 正智,大川 治夫他: "ブタ家族性横隔膜ヘルニアの病理学的遺伝学的検討" 日本小児外科学会雑誌. 26. 1118-1124 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松本 正智,大川 治夫他: "疾患モデルとしての鎖肛ブタ骨盤内神経分布の検討" 日本外科学会雑誌. 91. 151 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川 治夫,松本 正智,堀 哲夫: "直腸肛門奇形〜ブタモデル" 小児外科. 22. 499-505 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川 治夫,松本 正智: "ブタ鎖肛モデルを用いた研究" 外科. 53. 93-97 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川 治夫,池袋 賢一: "鎖肛ブタ胎仔における直腸肛門部の三次元立体構築" Mebio. 9. 104-114 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川 治夫,堀 哲夫: "新外科学大系30A小児外科I" 中山書店, 3-63 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] 大川 治夫: "産婦人科治療法の実際" 診断と治療社, 339-343 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo OHKAWA, Masatomo MATSUMOTO, Kenichi IKEBUKURO et al.: "Establishment of a pig model for ano-rectal malformations and its application to embryological studies." Japanese Journal of Pediatric Surgery. 22. 499-505 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masatomo MATSUMOTO, Haruo OHKAWA, Gohei OCHI et al: "Pathological and genetical studies on familial congenital diaphragmatic hernia in pig." J. JPN. SOC. PED. SURG.26. 1118-1124 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masatomo MATSUMOTO, Haruo OHKAWA et al: "Pelvic nerve distributions of pigs with ano-rectal malformations, as a disease model." J. JPN. SOC. PED. SURG.91. 151 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Haruo OHKAWA and Masatomo MATSUMOTO: "Studies with pig models with ano-rectal malformations." Geka. 53. 93-97 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Haruo OHKAWA and Kenxichi IKEBUKURO: "Three dimensional reconstruction of ano-rectum of foetuses of pig with ano-rectal malformations." MEBIO. 9. 104-114 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Haruo OHKAWA and Tetso HORI: Nakayama Shoten PBL.Characteristics of Pediatric Surgery, in Shin Gekagaku Taikei 30A, 3-63 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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