1990 Fiscal Year Annual Research Report

チトクロ-ムPー450の分子生物学、研究の総括

Research Project

Project/Area Number	02217101
Research Institution	Kyushu University
Principal Investigator	大村恒雄九州大学, 大学院医学系研究所, 教授 (80029933)
Co-Investigator(Kenkyū-buntansha)	奥田九一郎広島大学, 歯学部, 教授 (80034156) 渡辺民朗東北大学, 抗酸菌病研究所, 教授 (40006101) 加藤隆一慶応義塾大学, 医学部, 教授 (40112685) 藤井義明東北大学, 理学部, 教授 (00098146) 石村巽慶応義塾大学, 医学部, 教授 (40025599)
Keywords	チトクロ-ムPー450 / Pー450 / 総括班
Research Abstract	重点領域研究「チトクロ-ムPー450の分子生物学」の総括班として研究計画の検討、研究成果の評価とともに、研究全般の推進と研究成果の他分野の研究者への伝達のため下記の活動を行なった。 1. 総括班会議を3回開き、総括班の行事の立案、研究計画と研究の進行状況の検討を行なった。又、明年度に予定されている研究の取りまとめと報告書の作成についても相談した。 2. 12月6日に、東京で2つの計画研究班合同の研究班会議を行なった。会議には、総括班員と計画研究、公募採択研究の全研究者と共同研究者、合計85名が出席し、各研究者による研究発表と出席者全員による討論がなされた。 3. 総括班主催の公開講演会「Pー450の分子生物学:現状の総括と将来の展望」を12月7日に東京で開催した。講演会では、海外の研究者4名を含めて10名の演者が講演を行ない、活発な討論もなされた。講演会については、事前に「生化学」などの学術雑誌に広告を掲載した他、全国の主要な大学、研究機関にポスタ-を配布して他分野の研究者にも知らせるよう努力したが、181名の参加者があり盛会であった。 4. 研究班の全体会議に他に、特定の問題についての比較的小人数の研究集会も総括班で計画することとし、11月23日〜24日に「ステロイド代謝系のPー450の遺伝子とその発現調節」研究集会を広島で開催した。参加者は、研究班員と共同研究者、および招待した講演者を合わせて40名であった。 5.研究班員間の交流の便を考えて、昨年度に引き続き、全研究班員の連絡先と研究題目とともに研究経過、研究計画を記載した研究班員一覧表を作成し、全研究班員に配布した。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Shirou Kirita: "Structural Analysis of Multiple Bovine Pー450(11β)Genes and Their Promptor Activities" Journal of Biochemistry. 108. 1030-1041 (1990)
[Publications] Yoshikazu Emi: "A Different Cytochrome Pー450 Form Is Induced in Primary Cultures of Rat Hepatcytes" Proceedings of National Academy of Science,USA. 87. 9746-9750 (1990)
[Publications] Takashi Sato: "The Aminoーterminal Structures That Determine Topological Orientation of Cytochrome Pー450 in Microsomal Membrane" EMBO Journal. 9. 2391-2397 (1990)
[Publications] Y.Ohta: "Conversion of Cholesterol to Pregnenolone Mobilizes Cytochrome Pー450 in the Inner Membrane of Adrenocortical Mitochondria:Protein Rotation Study" Journal of Biochemistry. 107. 97-104 (1990)
[Publications] M.Noshiro: "Rat Liver Cholesterol 7αーHydroxylase" Journal of Biological Chemistry. 265. 10036-10041 (1990)
[Publications] Yamazoe,Y: "Modulation of hepatic level of microsomal testosterone 7αーhydroxylase Pー450a(Pー450IIA),by thyroid hormone and growth hormone in rat liver" Journal of Biochemistry. 108. 599-603 (1990)
[Publications] Nagata,K: "Purification and characterization of four catalytically active testosteron 6βーhydroxylase Pー450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms" Journal of Biochemistry. 107. 718-725 (1990)
[Publications] H.Ohkubo: "Generation of transgenic mice with elevated blood pressure by introduction of the rat renin and angiotensinogen genes" Proceedings of National Academy of Science,USA. 87. 5153-5157 (1990)
[Publications] Yokotani,N: "cDNA cloning of cytochrome Pー450 related to Pー450pー2 from the expression" European Journal of Biochemistry. 187. 23-29 (1990)
[Publications] Yanagida,A: "A novel cisーacting DNA element required for a high level of inducible expression of the rat Pー450c gene" Molecular and cellular Biology. 10. 1470-1475 (1990)
[Publications] N,Ueda: "Localization of arachidonate 12ーlipoxygenese in parenchymal cells of porcine anterior pituitary" Jounal of Biological Chemistry. 265. 2311-2316 (1990)
[Publications] T.Yoshimoto: "Cloning and sequence analysis of the cDNA for arachidonate 12ーlipoxygenase of porcine leukocytes" Proceeding of National Academy of Science,USA. 87. 2142-2146 (1990)
[Publications] N.Koga: "Purification and Characterization of Two Forms of 2,3,4,7,8ーPentaーchlorodibenzofuranーInducible Cytochrome Pー450 in Hamster Liver" Journal of Biochemistry. 107. 826-833 (1990)
[Publications] Shinーichirou Honda: "Novel cAMP Regulatory Elements in the Promotor Region of Bovine Pー450(11β)Gene" Journal of Biochemistry. 108. 1042-1049 (1990)
[Publications] Kenーichirou Morohashi: "TissueーSpecific Transcription of Pー450(11β)Gene In Vitro" Journal of Biochemistry. 108. 1050-1056 (1990)
[Publications] Y.Ohyama: "Cloning and expression of cDNA encoding 25ーhydroxyvitamin D_3 24ーhydroxylase" FEBS Letter. 278. 195-197 (1991)

1990 Fiscal Year Annual Research Report

チトクロ-ムPー450の分子生物学、研究の総括

Principal Investigator

大村 恒雄 九州大学, 大学院医学系研究所, 教授 (80029933)

Research Products

[Publications] Shirou Kirita: "Structural Analysis of Multiple Bovine Pー450(11β)Genes and Their Promptor Activities" Journal of Biochemistry. 108. 1030-1041 (1990)

[Publications] Yoshikazu Emi: "A Different Cytochrome Pー450 Form Is Induced in Primary Cultures of Rat Hepatcytes" Proceedings of National Academy of Science,USA. 87. 9746-9750 (1990)

[Publications] Takashi Sato: "The Aminoーterminal Structures That Determine Topological Orientation of Cytochrome Pー450 in Microsomal Membrane" EMBO Journal. 9. 2391-2397 (1990)

[Publications] Y.Ohta: "Conversion of Cholesterol to Pregnenolone Mobilizes Cytochrome Pー450 in the Inner Membrane of Adrenocortical Mitochondria:Protein Rotation Study" Journal of Biochemistry. 107. 97-104 (1990)

[Publications] M.Noshiro: "Rat Liver Cholesterol 7αーHydroxylase" Journal of Biological Chemistry. 265. 10036-10041 (1990)

[Publications] Yamazoe,Y: "Modulation of hepatic level of microsomal testosterone 7αーhydroxylase Pー450a(Pー450IIA),by thyroid hormone and growth hormone in rat liver" Journal of Biochemistry. 108. 599-603 (1990)

[Publications] Nagata,K: "Purification and characterization of four catalytically active testosteron 6βーhydroxylase Pー450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms" Journal of Biochemistry. 107. 718-725 (1990)

[Publications] H.Ohkubo: "Generation of transgenic mice with elevated blood pressure by introduction of the rat renin and angiotensinogen genes" Proceedings of National Academy of Science,USA. 87. 5153-5157 (1990)

[Publications] Yokotani,N: "cDNA cloning of cytochrome Pー450 related to Pー450pー2 from the expression" European Journal of Biochemistry. 187. 23-29 (1990)

[Publications] Yanagida,A: "A novel cisーacting DNA element required for a high level of inducible expression of the rat Pー450c gene" Molecular and cellular Biology. 10. 1470-1475 (1990)

[Publications] N,Ueda: "Localization of arachidonate 12ーlipoxygenese in parenchymal cells of porcine anterior pituitary" Jounal of Biological Chemistry. 265. 2311-2316 (1990)

[Publications] T.Yoshimoto: "Cloning and sequence analysis of the cDNA for arachidonate 12ーlipoxygenase of porcine leukocytes" Proceeding of National Academy of Science,USA. 87. 2142-2146 (1990)

[Publications] N.Koga: "Purification and Characterization of Two Forms of 2,3,4,7,8ーPentaーchlorodibenzofuranーInducible Cytochrome Pー450 in Hamster Liver" Journal of Biochemistry. 107. 826-833 (1990)

[Publications] Shinーichirou Honda: "Novel cAMP Regulatory Elements in the Promotor Region of Bovine Pー450(11β)Gene" Journal of Biochemistry. 108. 1042-1049 (1990)

[Publications] Kenーichirou Morohashi: "TissueーSpecific Transcription of Pー450(11β)Gene In Vitro" Journal of Biochemistry. 108. 1050-1056 (1990)

[Publications] Y.Ohyama: "Cloning and expression of cDNA encoding 25ーhydroxyvitamin D_3 24ーhydroxylase" FEBS Letter. 278. 195-197 (1991)

大村恒雄九州大学, 大学院医学系研究所, 教授 (80029933)